Ansam_00140 : CDS information

Location

Organism	Actinosynnema pretiosum subsp. auranticum
Strain	ATCC 31565
Entry name	Ansamitocin
Contig	AF453501
Start / Stop / Direction	30,306 / 28,981 / - [in whole cluster] 52,441 / 53,766 / + [in contig]
Location	complement(28981..30306) [in whole cluster] 52441..53766 [in contig]
Type	CDS
Length	1,326 bp (441 aa)

Annotation

Category	3.4 other modification
Product	halogenase
Product (GenBank)	halogenase
Gene
Gene (GenBank)	asm12
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q8KUG0 PmId [12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002) [14624546] The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. (J Am Chem Soc. , 2003) comment [PMID:12060743](2002) Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。 <cluster I> Asm12(441aa) : Halogenase asm12の不活化mutant HGF054はansamitocin P-3非産生であり、ひとつの化合物ではなく一連の19-deschloro-ansamitocin派生体を蓄積した(unpublished work)。よってAsm12はC-19でchlorineを導入するhalogenaseであり、そのhalogenの存在は他のPKS後修飾反応のいくつかには絶対的な要求をされないと示唆された。 --- [PMID:14624546](2003) Actinosynnema pretiosumのansamitocin生合成gene clusterにある6genesの機能を、遺伝子不活化とmutantの化学分析によって調査。それらはそれぞれ halogenase (asm12), carbamoyltransferase (asm21), 20-O-methyltransferase (asm7), 3-O-acyltransferase (asm19), epoxidase (asm11), N-methyltransferase (asm10) をコードし、ansamitocin形成において6つのPKS後修飾ステップを担う。 asm12 insertion mutantは19-deschloro-N-demethyl-ansamitocin P-3をはじめとする多くのdeschloro化合物を蓄積した。 proansamitocinからの1st stepであると提唱されている。

Sequence

>halogenase [halogenase]
MLDAIVMGGGPAGSVCAAVLARQGRSVLVLERQEFPRFHIGESMLPYMVGLLERHGLLDA
VREQGYVVKRGGEFIDPTGTKFFRAGVFRADFAKTGDGRHHETFQVERSHFDRVNLDQAR
AAGATVREGAQVVGLLEEGGRVVGVRYREGGVEREERARYVVDATGRAGVVANRFGLRRM
IEDLRMVAVFHHRDGLDEAHNPGHEGDIQVGSHSDGWIWAIPLSADRISVGTVMHRDRLR
GRTPAEAFAEHVERVPRINQRLTGTSATSDFWVETDYSYHSDQVTGPGWVMVGDAGCFGD
PMFSGGVLVGMATGAEAAEALGRALDSPADEEQALTGYSNFFKTGYDTYVRLIFSFYEGE
LLPALAEAHSEAGNLSEADMEMYVVRLLGGDFWSARNPVANALRANPAWSTFSPFEPVHR
CPVYPELDVAELGGLAGTVGR

>halogenase [halogenase]
ATGCTCGACGCGATCGTGATGGGTGGTGGGCCCGCCGGGTCCGTCTGCGCCGCGGTGCTC
GCCCGGCAGGGCCGCTCGGTGCTGGTGCTGGAGCGGCAGGAGTTCCCCCGCTTCCACATC
GGCGAGTCGATGCTGCCGTACATGGTGGGCCTGCTGGAGCGGCACGGCCTGCTCGACGCG
GTGCGGGAGCAGGGCTACGTCGTCAAGCGCGGCGGCGAGTTCATCGACCCGACCGGCACG
AAGTTCTTCCGCGCCGGGGTGTTCCGCGCCGACTTCGCCAAGACCGGCGACGGCCGCCAC
CACGAGACGTTCCAGGTCGAGCGCTCGCACTTCGACCGGGTGAACCTGGACCAGGCGCGC
GCGGCGGGCGCGACCGTGCGGGAGGGCGCGCAGGTCGTCGGGCTGCTGGAGGAGGGCGGC
CGGGTGGTGGGCGTGCGCTACCGCGAGGGCGGGGTCGAGCGCGAGGAGCGCGCCCGGTAC
GTGGTGGACGCCACCGGCCGGGCGGGCGTGGTCGCCAACCGGTTCGGGCTGCGGCGGATG
ATCGAGGACCTGCGCATGGTGGCGGTGTTCCACCACCGCGACGGGCTGGACGAGGCGCAC
AACCCCGGCCACGAGGGCGACATCCAGGTCGGCAGCCACTCGGACGGGTGGATCTGGGCG
ATCCCGCTGTCGGCGGACCGGATCAGCGTCGGCACGGTCATGCACCGCGACCGGCTGCGC
GGGCGCACCCCGGCCGAGGCGTTCGCCGAGCACGTCGAGCGGGTGCCCAGGATCAACCAG
CGGCTCACCGGCACCTCCGCGACCTCGGACTTCTGGGTGGAGACCGACTACAGCTACCAC
TCCGACCAGGTCACCGGCCCCGGCTGGGTGATGGTCGGGGACGCCGGCTGCTTCGGCGAC
CCCATGTTCTCCGGCGGGGTGCTGGTGGGCATGGCCACGGGCGCGGAGGCCGCCGAGGCG
CTCGGGCGGGCGCTCGACTCGCCCGCCGACGAGGAGCAGGCGCTCACCGGCTACTCGAAC
TTCTTCAAGACCGGCTACGACACGTACGTGCGGCTGATCTTCTCCTTCTACGAGGGCGAG
CTGCTGCCCGCGCTGGCCGAGGCGCACTCGGAGGCGGGGAACCTCTCCGAGGCGGACATG
GAGATGTACGTGGTGCGGCTGCTCGGCGGCGACTTCTGGAGCGCGCGCAACCCGGTGGCG
AACGCGCTGCGCGCGAACCCGGCGTGGTCGACGTTCTCGCCGTTCGAGCCGGTGCACCGC
TGCCCGGTGTACCCCGAGCTGGACGTGGCGGAGCTGGGCGGGCTCGCGGGCACGGTCGGC
CGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE IPR006905 Tryptophan halogenase (Family) PF04820 Trp_halogenase
SignalP	No significant hit
TMHMM	No significant hit

Ansam_00130 Ansam_00150