Ansam_00150 : CDS information

Location

Organism	Actinosynnema pretiosum subsp. auranticum
Strain	ATCC 31565
Entry name	Ansamitocin
Contig	AF453501
Start / Stop / Direction	30,563 / 31,588 / + [in whole cluster] 52,184 / 51,159 / - [in contig]
Location	30563..31588 [in whole cluster] complement(51159..52184) [in contig]
Type	CDS
Length	1,026 bp (341 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative oxidoreductase
Product (GenBank)	3-hydroxyacyl-CoA dehydrogenase
Gene
Gene (GenBank)	asm13
EC number
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)
Reference	ACC Q8KUG1 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) [12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002) comment [PMID:11996558](2002) abstract S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6-deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。また、methyltransferaseをコードするasm17を削除すると、そのhydroxy類似体ではなく6-deoxyerythronolide Bが得られたので、伸長unitのmethylationはその取り込みのために必要である。 --- [PMID:12060743](2002) Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。 <cluster I> Asm13(341aa) : 3-Hydroxyacyl-CoA Dehydrogenase(Function of homologue) Asm13はNAD-dependent 3-hydroxybutyryl-CoA dehydrogenaseに似ている。 asm13-17はオペロンを形成。asm13 と asm14はoverlapsがあるので転写共役するかもしれない。稀なpolyketide鎖伸長unit methoxymalonayl-ACPの形成に関連すると提唱されている。
Related Reference	ACC Q9ADL9 NITE Soraph_00040 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment Blast 305th, id41%, 2e-39 Sorangium cellulosum_sorD Beta-hydroxybutyryl-CoA dehydrogenase --- [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。本ORFはCH2OHをCHOにする。 --- [PMID: 15289572](2004) sorC破壊株はsoraphen A非産生。 S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
	ACC Q7BQ74 PmId [16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006) comment Blast 163rd, id36%, 9e-43 Bacillus cereus_zmaG ZmaG ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。 ZmaE or ZmaIと共に働く。 ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP) ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
	ACC P52041 PmId [8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996) comment Blast 371st, id35%, 2e-38 Clostridium acetobutylicum_hbd 3-hydroxybutyryl-CoA dehydrogenase beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase (BHBD)の遺伝子のクローニングを行い、E. coliで過剰発現させたところBHBDの細胞外への分泌を確認。また、C. acetobutylicumで発現させ、BHBD activityの上昇を確認している。

Sequence

>putative oxidoreductase [3-hydroxyacyl-CoA dehydrogenase]
MPGDTDDPPARALASPSPDGPPPDGPSSLGPSSGGMSPGGAARGGASPGEPGRPAGERPL
LVLGAGVMGCGITALALSRGLPVLLVDPDADRLDAARADVRAHLRTAQLLGVAAGPLGEL
TTATDTGGPREVVAVVEAVTEDAETKAKALTGVCATVPPGTPLVSNTSSIPMGELAPALP
RPGDLVGAHFMNPPYLIPAVEVARGPLTSDAAFAGLTALLARLGRAPVQVGDAPGFVTSR
LLHPMINDAARVVESGTADAAAVDALMRDCLGHREGPLRTADLIGIDNLVDSLRVLRRRT
GDERCEPCDLLLAKVDAGHLGRKSGRGFYDYGHPGREGTGR

>putative oxidoreductase [3-hydroxyacyl-CoA dehydrogenase]
GTGCCCGGCGACACCGACGACCCACCGGCCCGAGCGCTCGCCAGCCCGTCACCCGACGGC
CCGCCACCCGACGGCCCGTCGTCCCTCGGTCCGTCCTCCGGCGGCATGTCGCCCGGCGGC
GCGGCGCGCGGCGGCGCGTCGCCCGGCGAACCGGGCCGCCCGGCCGGGGAGCGCCCGCTG
CTGGTGCTGGGCGCGGGCGTCATGGGCTGCGGGATCACCGCGCTCGCGCTCTCCAGGGGC
CTGCCCGTGCTCCTGGTGGACCCGGACGCCGACCGGCTCGACGCGGCCCGCGCCGACGTG
CGCGCGCACCTGCGGACCGCCCAGCTGCTCGGCGTCGCCGCCGGGCCGCTCGGCGAGCTG
ACCACCGCGACCGACACGGGCGGCCCCCGCGAGGTGGTCGCCGTGGTCGAGGCGGTCACC
GAGGACGCCGAGACCAAGGCCAAGGCGCTCACCGGGGTCTGCGCCACCGTCCCGCCGGGC
ACCCCGCTGGTCTCCAACACCTCCTCCATCCCCATGGGCGAGCTGGCCCCCGCGCTGCCC
AGGCCCGGCGACCTCGTGGGCGCGCACTTCATGAACCCGCCGTACCTGATCCCCGCCGTC
GAGGTAGCGCGCGGCCCGCTGACCTCGGACGCGGCGTTCGCCGGGCTCACCGCCCTGCTC
GCGCGCCTCGGCCGCGCCCCGGTACAGGTCGGCGACGCGCCGGGCTTCGTCACCAGCCGC
CTGCTGCACCCGATGATCAACGACGCGGCGCGCGTCGTGGAGTCGGGCACCGCCGACGCC
GCCGCCGTCGACGCGCTCATGCGCGACTGCCTCGGCCACCGCGAGGGCCCGCTGCGCACC
GCCGACCTGATCGGCATCGACAACCTCGTCGACTCGCTGCGCGTGCTGCGCAGGCGCACC
GGGGACGAGCGGTGCGAGCCGTGCGACCTGCTGCTGGCCAAGGTCGACGCGGGGCACCTC
GGGCGCAAGAGCGGCCGGGGCTTCTACGACTACGGCCACCCCGGCCGTGAAGGGACGGGG
CGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	No significant hit
TMHMM	No significant hit

Ansam_00140 Ansam_00160