Ansam_00230 Ansam_00230   Ansam_00250 Ansam_00250

Ansam_00240 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction42,323 / 43,222 / + [in whole cluster]
40,424 / 39,525 / - [in contig]
Location42323..43222 [in whole cluster]
complement(39525..40424) [in contig]
TypeCDS
Length900 bp (299 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative kanosamine kinase
Product (GenBank)kinase
Gene
Gene (GenBank)asm22
EC number
Keyword
  • AHBA
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm22(251aa) : Kinase

配列解析のみ。
PKS starter AHBAの生合成genesのひとつとされている。
Related Reference
ACC
O52554
NITE
Rifam_00340
PmId
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
[12207505] Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. (J Am Chem Soc. , 2002)
comment
Blast 16th, id50%, 4e-37
Amycolatopsis mediterranei_rifN
RifN
[DoBISCUIT]kanosamine kinase

---
[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

どちらもRifN: kinase

rifNは、PKSのstarter unitであるAHBAの合成に必要十分な7genes(rifG, H, J, K, L, M, N)のひとつだが、rifH, G, Jと異なりshikimate pathway関連遺伝子には似ていない。

rifN mutantの実験から、AHBA生合成の早期段階で作用し、aminoDAHP形成か、それより前で関与することが示唆されている。

---
[PMID: 12207505](2002)
rifNについての実験論文。

E.coliでHis6 fusion proteinとして過剰発現、精製したRifNの基質特異性を様々な糖で確認。kanosamineのみが反応し、この反応生成物をNMRなどで解析したところ、kanosamine 6-phosphateと同定された。

よって、RifNはkanosamine kinase
kanosamine → kanosamine 6-phosphate

close this sectionSequence

selected fasta
>putative kanosamine kinase [kinase]
MSGSGTGGVVGVDLGGTKAAVRVVGADGRSGDSVVRWESGASAGEDLALLVRAVERAGGG
GGVRSVGVAVPATLDRSGVVVAWPGRPGWVGVDVLGALGAMFPGASVRHGDDGDLAALAE
ADAAGCGELLYVGVGTGVGGGIVSGGLPLNSGGSCEVGHVVVERGGGLCDCGRRGCVQAF
ASGPAILRGAVARGGRPGGLGDVRDGVARGERWAVEACEDAASALAALVIGVGELVRPEL
ALIGGGVTTALPGLVPAVAERVAGLGRTGHPVPSVRPAVLGAESSLHGALVLARGGNRE
selected fasta
>putative kanosamine kinase [kinase]
GTGAGCGGTTCGGGTACTGGCGGGGTGGTTGGAGTTGATCTTGGGGGGACGAAGGCCGCT
GTTCGGGTTGTTGGTGCGGACGGGCGGTCGGGCGACAGCGTGGTGCGGTGGGAGAGCGGC
GCTTCTGCTGGCGAAGACCTCGCCTTGCTCGTTCGGGCGGTCGAGCGGGCTGGTGGGGGT
GGGGGTGTCAGGTCGGTTGGTGTCGCTGTGCCTGCCACGCTCGATCGGTCCGGCGTCGTG
GTCGCCTGGCCGGGTCGGCCCGGGTGGGTCGGGGTGGACGTGCTCGGTGCGTTGGGGGCG
ATGTTCCCCGGCGCCTCGGTGCGGCACGGGGATGACGGTGACCTCGCGGCGCTTGCCGAG
GCCGATGCCGCCGGGTGCGGTGAGCTGCTGTACGTCGGTGTGGGCACCGGGGTTGGTGGC
GGGATCGTCTCGGGTGGACTGCCGCTGAACTCGGGTGGCTCGTGCGAGGTGGGGCACGTG
GTCGTCGAACGGGGTGGGGGGCTGTGCGACTGCGGGCGGCGGGGGTGCGTGCAGGCGTTC
GCGTCCGGGCCCGCGATTCTGCGCGGGGCCGTCGCGCGTGGTGGGCGGCCCGGTGGGCTG
GGGGACGTCCGGGACGGGGTCGCGCGTGGGGAGCGGTGGGCGGTGGAGGCTTGTGAGGAC
GCCGCGTCCGCGTTGGCCGCGCTGGTGATCGGGGTGGGGGAGCTGGTTCGGCCCGAGTTG
GCGCTGATCGGCGGTGGTGTCACCACCGCGCTGCCAGGGCTCGTGCCCGCTGTGGCCGAG
CGGGTCGCCGGGCTGGGCAGGACCGGGCACCCGGTGCCATCGGTGCGACCGGCGGTGCTC
GGGGCCGAGTCCTCGCTGCACGGGGCGCTTGTCCTGGCGCGTGGGGGAAACCGGGAGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000600 ROK (Family)
[11-186] 8.8e-20 PF00480
PF00480   ROK
SignalP No significant hit
TMHMM No significant hit
Ansam_00230 Ansam_00230   Ansam_00250 Ansam_00250
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