Ansam_00280 Ansam_00280   Ansam_00300 Ansam_00300

Ansam_00290 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction69,084 / 70,292 / + [in whole cluster]
13,663 / 12,455 / - [in contig]
Location69084..70292 [in whole cluster]
complement(12455..13663) [in contig]
TypeCDS
Length1,209 bp (402 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductUDP-glucose N-glycosyltransferase
Product (GenBank)glycosyltransferase
Gene
Gene (GenBank)asm25
EC number
Keyword
  • derivative
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[18721757] Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins. (Chem Biol. , 2008)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm25(402aa) : Glycosyltransferase

配列解析のみ。
過去文献を引用してasm41と共にglycosylation/deglycosylation excretion systemの一部かもしれないと述べられているが、後に別の機能がわかっている。

---
[PMID:18721757](2008)
N-methyl基の代わりにmacrolactam amide nitrogenに付着したbeta-D-glucosyl基を持っているansamitocinoside P-3 (AGP-3)を分離。

このmacrolactam amide N-glycosylationをAsm25が担うことを、遺伝子不活化、相補により同定。

また、E.coliで発現して得られたAsm25タンパクを用いて最適反応条件、基質特異性について調査。
基質として最も好まれたのはN-desmethyl-ansamitocin P-2。いくつかのansamycinsと合成indolin-2-onesも基質として受け入れられるが、糖はUDP-Gluしか使えない。

各産物のAntifungal and Antitumor活性も測定している。

close this sectionSequence

selected fasta
>UDP-glucose N-glycosyltransferase [glycosyltransferase]
MRVLFTVTGAPSHAHEMLVLGRAVLDAGHEVLVATAPSAAPGLRGSGLPSVEALPDQARF
WVERLGGVAPPARPGTDYPGVAAIVDVIAGRPLLREALTGLRPVVDRFRPDLVLRDGMEL
AGLLVAEERGLPCLTVPSGTTNQLDPAAVLPRLNERRAEQGLPTSADPLGAHRHGHVDCL
PREYSLLGVPLSPCLAYRPPSPDGRSLPAWVADLDPSRPLVYGAVGIALAALWRRDDVTA
LVADPVEALGGLVAALSEVDCAAVVSTGGIPVDRVPPGAPHVRVVERAPQPLLLECAQLF
VTHAGANSVREAVRAGVPMVAAPLFGDQPHNAARVAALGLGVRVVDGSPADLADACRTVL
ADPRFTARARHARRTLLALPPVGEVVTRLEQIADAARSVAVV
selected fasta
>UDP-glucose N-glycosyltransferase [glycosyltransferase]
TTGCGCGTTCTGTTCACCGTCACCGGGGCCCCGTCGCACGCGCACGAGATGCTCGTGCTC
GGGCGCGCGGTCCTGGACGCCGGGCACGAGGTGCTCGTGGCCACGGCCCCCAGCGCCGCG
CCCGGCCTGCGGGGCAGCGGCCTGCCCTCGGTCGAGGCGTTGCCCGACCAGGCGAGGTTC
TGGGTGGAGCGGCTCGGGGGCGTCGCGCCACCGGCCAGGCCGGGCACGGACTACCCCGGC
GTCGCCGCGATCGTCGACGTCATCGCGGGCAGGCCGCTGCTGCGGGAGGCGCTGACCGGG
CTGCGCCCCGTCGTCGACCGCTTCCGGCCCGACCTGGTCCTGCGCGACGGCATGGAGCTG
GCCGGCCTGCTCGTCGCGGAGGAGCGCGGGCTGCCCTGCCTGACCGTGCCCTCGGGCACG
ACGAACCAGCTGGACCCGGCCGCCGTGCTGCCCCGGCTCAACGAGCGCCGCGCCGAGCAG
GGCCTGCCCACCTCGGCCGACCCGCTCGGCGCGCACCGGCACGGCCACGTCGACTGCCTG
CCGCGCGAGTACTCGCTGCTCGGCGTCCCGCTCAGCCCGTGCCTGGCCTACCGGCCGCCG
TCGCCCGACGGCCGGTCGCTGCCCGCGTGGGTGGCCGACCTGGACCCGTCCCGCCCGCTG
GTGTACGGGGCGGTCGGCATCGCGCTGGCCGCCCTGTGGCGCCGCGACGACGTGACCGCG
CTGGTCGCCGACCCGGTGGAGGCGCTGGGCGGACTCGTGGCGGCCCTGTCCGAGGTGGAC
TGCGCCGCGGTGGTGTCCACCGGGGGCATCCCGGTCGACCGCGTCCCGCCGGGCGCGCCG
CACGTGCGCGTGGTCGAGCGGGCGCCCCAGCCGCTGCTGCTGGAGTGCGCGCAGCTGTTC
GTCACCCACGCCGGGGCCAACAGCGTGCGCGAGGCGGTGCGGGCGGGGGTGCCCATGGTC
GCCGCCCCGCTGTTCGGCGACCAGCCGCACAACGCCGCCAGGGTCGCCGCGCTGGGGCTG
GGCGTGCGGGTGGTGGACGGCTCGCCCGCCGATCTGGCGGACGCCTGCCGGACCGTGCTG
GCCGATCCCCGCTTCACCGCGAGGGCGCGCCACGCGCGCCGGACCCTGCTCGCGCTGCCC
CCGGTGGGCGAGGTGGTGACCCGCCTGGAGCAGATCGCCGACGCCGCGCGGAGCGTCGCG
GTGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family)
[282-365] 6.40000000000001e-19 PF00201
PF00201   UDPGT
SignalP
[1-14] 0.689 Signal
Eukaryota   
[1-14] 0.33 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Ansam_00280 Ansam_00280   Ansam_00300 Ansam_00300
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