Ansam_00270 Ansam_00270   Ansam_00290 Ansam_00290

Ansam_00280 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction59,785 / 69,006 / + [in whole cluster]
22,962 / 13,741 / - [in contig]
Location59785..69006 [in whole cluster]
complement(13741..22962) [in contig]
TypeCDS
Length9,222 bp (3,073 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase
Gene
Gene (GenBank)asmB
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[19292483] Timing of the Delta(10,12)-Delta(11,13) double bond migration during ansamitocin biosynthesis in Actinosynnema pretiosum. (J Am Chem Soc. , 2009)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
AsmB(3073aa, 313.2 kDa) Polyketide synthase
Module 3 : KS AT DH KR ACP
Module 4 : KS AT ACP

asmB deletion mutantではansamitocin P-3やansamitocin派生体の産生なし。よって、このcluster I はansamitocin形成に必要であることが確認された。

S. coelicolorでの異種性発現において、
asmA + rif AHBA合成genes = 関連産物なし
asmAB + rif AHBA合成genes = N-acetyl triketide蓄積

よってAsmAの産物は次のKSに移動されるときだけその同族のACP2から放出されうるが、基質(glycolate unit)を欠いているためさらに加工されないと示唆される。

--
3-amino-5-hydroxybenzoic acid (AHBA)はstarter unit.

---
[PMID:19292483](2009)
AHBA非産生mutant A. pretiosumをSNAC thioestersで相補することで、普通でない配置のDelta(11,13) diene systemが導入されるタイミングを確認している。

この結果から、asmPKS module 3での新生polyketide加工の際に設置され、ansamitocin P-3と同じ配置になったものしかKS4は受け入れないことがわかった。

最終的なdiene systemは、vinylogous syn-eliminatinによって、module 3 のDHでのdehydration stepの際に設置されると提唱している。

close this sectionPKS/NRPS Module

3 methoxymalonyl-ACP
4 malonyl-CoA
KS29..404
AT646..959
DH1004..1158
KR1410..1584
ACP1663..1735
KS1759..2131
AT2288..2571
ACP2916..2989

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase]
MTTREQLLDALRASLRENERLRATGGAAEPVAIVGAACRFPGGVTSPEELWDLVVGEVDA
AGPFPGDRGWDLADLFDPDPDRVGRSYVRHGAFLRDMAWFEPGFFGISPREALVIDPQHR
LLLEVAWEAFERAGIAPDSVRGRDVGVFGGVLSHDHGSRFRPVPPELEGYLGTGSSGSAA
IGRVAYTLGLEGPAITVDTACSSSLVAIHLAVRALQRGECSMALAGGVTVMSTPDTFVEF
SRQRALARDGRCKAFSADADGTAWGEGAGLVLLQPLSAARRDGRRVLAVVRGSAVNSDGA
SNGLTAPSGPAQQRVIRAALADAGLSAADVDLLEAHGTGTVLGDPIEAQALLETYGRGRA
GAGPLWLGSVKSNIGHTQGAAGVAGVLKVVQALRNGVLPPTLHAATPTTEVDWDAGAVRV
LDRALPWRGGGEPGSALGDAAGGLEGGAAGGRPGGAAGGVAGGVGGGAAAVRLRRGAVSA
FGASGTNAHLILEEAPVAEAVAEAVAPATTGSPAAPGAVGAPGVTAAPGTFAATAAVPSA
PLPAAASDAPVPVPVPAPVPVVVSGATTAGLRAQAARLAGHLRERPALGPEDVARPLLLS
RAQRERRAVVVARDREALLGGLDALAGGEAGRRLVSGAADVTGRVVLVFPGQGAHWTGVA
ERLWREAPAFADSMARCADVLRDLAGWELREVLVDPVALERVDVLQPVSFAVVVSLAALW
ASVGVRPDAVVGHSQGEVAAAHVAGALTLAEAARIVVLRSALIARELSGRGAMLTVVADV
ERVTALLAGFEGRVCVAAVNGPASVTVSGEDAAVREFERVLSARRVLRWRLPGVDFAGHS
PQVDALRAELLAALGDVAAREPEIPLLSTVTGRPATRLDAEHWYRNLREPVRFADAVTAL
LDRGHRVFVEVSPHPVLTTSVVDLAAPHRTAVVGTLRRDEGGLDRFLLSAGELHVRGVPV
DLARHAGAGTAEVPTTVFQRERYWLDAPRPSGDARAAGLVPSGHPLLGALVPLADGAVLT
GRLSLSAQPWLADHRVLGAVVVPGVALVELAVRAGDEVGCPALEELVVAAPLVVPESGDV
AVRVVVGPRGADGTRPVDVLSGDQPPVLHARGVLGPPRAPEPVGGQWPPAGAEPVDLAGL
YPDFAAKGLEYGPHFQRLTRLWRRGDEVFGEVELTGEQRRAAGFALHPALLECALHVSSF
TGQRVAGDGGAVGLPFSFSRVALHRSGVDAVRVRAARVGDGVVSLDLADGAGRPVARIGE
LAFRRAGGIRSGPPGVLLETAWRPVELPEAPPPADVTTVVGDSVERVLAALRETLAGDGR
LLVLTGDPGAPIGTAVGGAVWGLVRSAQLEEPGRIVLADLDTAPERAGAVLDRVLASGEP
QVAIRGGAVLVPRLTPVPVVAGPAGLDPAKTVVLTGGTGALGRLVARHLVTEHGARRLLL
LGRGGPERARDLVAELTGLGAEVEALACDVADRGALSTALAGRALTAVVHLAGALDDGVV
TALTPERLAAAFAPKALAARHLDELTRDQDLAAFVLFSSVASVLGSAGQANYAAANGFLD
GLAHERHALGLPATSIAWGLWDVDSALVGERGAADRERLAASGMRPLPAPAALDLLDAAL
ACGRPAVTALALARAADRAPAALGEVVASRRAPAPAAPVPGGPAALLALVRAEAAAVLAL
PVAGVDERRAFRDLGVDSLTGVELRNRLAEATGLRLPATLVFDHPTPVELAAALLERVGG
TSGPAGGASAPAPASAPGEPIAIVGMACRAPGGVASPEDLWDLVAGGVDAVGPFPADRGW
DLERLVHPDPDHEGSTYVDRAAFLDDPAGFDADFFGISPREALAMDPQQRLLLEVAWEAF
ERAGIAPDSVRGTDVGVFAGVTNHDYDQVLPRGDDGYRITGVSGSVVSGRIAYALGLEGP
ALSVDTACSASLVAIHLAVRALRGGECSMALAGGATVMATPRGFVEFARQRGLAPDGRCK
PFSASADGTAWSEGAGLVLLERLSDARRNGRRVLAVVRGSAVNSDGASNGLTAPSGAAQQ
RVIRAALADAGLSTSDVDAVEAHGTGTRLGDPIEARALVATYGRDRDGEPLWLGSLKSNL
GHMQGAAGIGGVVKTVQALRHGVLPRTLHVDAPTPEVEWDGVALLAEQRAWPEVDRPRRA
AVSSFGVSGTNAHVVLEQAPAERTAAENPGGNAAGRVGDFPGVLPLVLSARSPAALRAQA
ARLADVLDDLPLRDVASTLARGRAAHEHRAVLVAADRDALRAGLRALAGGRPHAAARTGV
RAPGGLAFAFSGQGSQFVGMGRGLHAAFPAFAEVFDRVCAALDERLGELTGRSVRDVVLD
GPAELLAETVHAQPALFALEVALARLLEGWGVVPDVVLGHSVGELAAAHVAGALSEADAC
ALVAARARAMHGARRGGAMLAVSATEEQVAPLLGGGLALAAVNGPGAVVLSGDGDAVERA
AAECAGRGWRTRALRVSHAFHSAHLDGVLGELAVAARRVEVRPPRVPLLSTLTGAPADVE
LLADPDYWARQAREAVRFGDGVARLGELGVTRVLEVGPGGALAALVREQVAPGAAVARGL
VDGDEVVGLLSGIGDLFASGAPVDWTALLGGPGAVVDLPTYPFQRTRFWPAAQAPGAWCE
VRLERLPAPRGGGSATVLEVGPTASAEELASVADVLAEHSGPLLVVTGDGAVAGLVRSLP
DRADVVLADVDDDPASVALLPALHGCGEPELVVRAGEVLAPRLRRVTATGAAPSGPVLVV
GEAARGVVAACGVREPVVLPADSGRAELVAALDDHRPDWVVLAVAPDDAGLALAALLDEL
TRDRPPALFALCGPAGALGDAARPEDAVAAGVLAALVRRRRGDGLPATLLCAGPGTPPLS
PELGVVVFARTPEVVLPEPRTAQPEPDAVDLTGPDRDRVLLALVRDHAAAVLGRADGPVD
GRAAFRDLGFDSMTAVELRDRLAAATGLTLPATLLFDHPTAAEVAAVLAGLLDGGDPVTS
GLDRLEAALADPPADPALRASAAARLRALLAALSDEGDGRDGAGQDGAAALRDAGVAELF
EFIDTRLGRAERA
selected fasta
>polyketide synthase [polyketide synthase]
GTGACCACCCGTGAGCAGCTCCTGGACGCGCTGCGCGCCTCGCTGCGGGAGAACGAGCGG
CTGCGCGCCACCGGGGGCGCGGCCGAGCCGGTCGCGATCGTCGGCGCGGCCTGCCGCTTC
CCCGGCGGGGTCACCTCGCCCGAGGAGCTGTGGGACCTGGTCGTCGGCGAGGTCGACGCG
GCGGGCCCGTTCCCAGGCGACCGGGGCTGGGACCTGGCGGACCTGTTCGACCCCGACCCC
GACCGGGTCGGGCGCTCCTACGTGCGGCACGGGGCGTTCCTGCGCGACATGGCCTGGTTC
GAGCCGGGGTTCTTCGGGATCTCGCCGCGCGAGGCCCTGGTGATCGACCCGCAGCACCGG
CTGCTGCTGGAGGTCGCGTGGGAGGCGTTCGAGCGCGCGGGCATCGCCCCGGACTCGGTG
CGCGGGCGCGACGTCGGCGTCTTCGGCGGCGTGCTCAGCCACGACCACGGCTCGCGGTTC
CGGCCCGTGCCGCCGGAGCTGGAGGGCTACCTGGGGACCGGCAGCTCCGGCAGCGCCGCG
ATCGGGCGCGTCGCCTACACCCTCGGGCTGGAGGGGCCCGCGATCACGGTGGACACCGCC
TGCTCGTCGTCGCTGGTCGCGATCCACCTCGCGGTGCGGGCGCTCCAGCGCGGCGAGTGC
TCGATGGCGCTGGCGGGCGGGGTGACGGTGATGTCGACGCCGGACACGTTCGTCGAGTTC
TCGCGGCAGCGCGCGCTGGCCCGCGACGGGCGCTGCAAGGCGTTCTCCGCCGACGCGGAC
GGCACCGCGTGGGGCGAGGGCGCGGGTCTGGTGCTGCTGCAACCGCTCTCCGCCGCCCGG
CGGGACGGGCGGCGGGTGTTGGCGGTGGTGCGCGGGTCGGCGGTGAACTCCGACGGCGCG
TCCAACGGGCTCACCGCGCCCAGCGGTCCCGCGCAGCAGCGGGTGATCCGGGCGGCGCTG
GCCGACGCCGGGCTGTCGGCGGCGGACGTGGACCTGCTGGAGGCGCACGGCACCGGGACC
GTGCTCGGGGACCCGATCGAGGCGCAGGCGCTGCTGGAGACCTACGGGCGCGGTCGCGCG
GGCGCCGGGCCGCTGTGGCTCGGGTCGGTGAAGTCGAACATCGGGCACACGCAGGGCGCG
GCGGGCGTGGCCGGGGTGCTGAAGGTGGTGCAGGCGCTGCGCAACGGCGTCCTGCCCCCC
ACCCTGCACGCCGCGACGCCGACCACCGAGGTCGACTGGGACGCCGGGGCGGTGCGGGTG
CTCGACCGGGCCCTGCCTTGGCGTGGGGGAGGGGAGCCAGGGAGTGCACTGGGGGATGCT
GCTGGTGGCTTGGAGGGTGGTGCTGCGGGGGGCCGACCGGGTGGTGCTGCCGGGGGCGTG
GCGGGTGGAGTCGGTGGGGGCGCCGCGGCGGTGCGGCTGAGGCGGGGGGCGGTGTCGGCC
TTCGGGGCCAGCGGCACCAACGCCCACCTGATCCTCGAAGAGGCGCCCGTGGCGGAGGCC
GTGGCGGAGGCCGTGGCACCCGCCACGACCGGCTCACCTGCCGCGCCCGGCGCGGTTGGC
GCACCGGGCGTGACCGCCGCGCCCGGAACCTTCGCCGCGACCGCTGCCGTGCCCTCTGCG
CCGCTGCCCGCTGCCGCGTCCGATGCCCCCGTGCCTGTGCCCGTGCCCGCCCCCGTCCCG
GTCGTCGTGTCCGGGGCCACCACCGCCGGTCTGCGCGCCCAGGCGGCGCGCCTGGCCGGG
CACCTGCGGGAGCGGCCCGCGCTCGGCCCCGAGGACGTCGCCCGGCCGCTGCTGCTCTCC
CGCGCCCAGCGCGAGCGGCGCGCCGTCGTGGTCGCCCGCGACCGGGAGGCGCTGCTCGGC
GGGCTCGACGCCCTCGCGGGTGGTGAGGCCGGGCGCCGGCTCGTGAGCGGCGCGGCCGAC
GTCACCGGGCGGGTGGTCCTGGTGTTCCCCGGTCAGGGCGCGCACTGGACCGGCGTGGCC
GAGCGGTTGTGGCGGGAGGCGCCCGCCTTCGCCGACAGCATGGCCCGCTGCGCCGACGTG
CTGCGCGACCTCGCCGGGTGGGAGCTGCGGGAGGTGCTCGTCGACCCGGTCGCGCTGGAG
CGCGTCGACGTGCTGCAACCGGTCTCGTTCGCGGTCGTCGTGTCGCTCGCGGCGCTCTGG
GCGTCGGTCGGCGTGCGGCCCGACGCGGTCGTCGGGCACTCGCAGGGCGAGGTGGCCGCC
GCCCACGTCGCGGGCGCGCTCACGCTCGCCGAGGCCGCCCGGATCGTCGTGCTCCGCTCC
GCGCTGATCGCCCGCGAGCTGTCCGGCCGCGGCGCGATGCTCACCGTCGTCGCCGACGTC
GAGCGGGTCACCGCGCTGCTCGCCGGGTTCGAGGGGCGGGTGTGCGTGGCGGCCGTCAAC
GGGCCCGCGTCGGTCACCGTCTCCGGCGAGGACGCGGCGGTGCGCGAGTTCGAGCGGGTC
CTGTCCGCCCGGCGGGTGCTGCGGTGGCGGCTGCCCGGCGTCGACTTCGCCGGGCACTCC
CCGCAGGTCGACGCCCTGCGCGCGGAGCTGCTGGCCGCACTCGGCGACGTCGCCGCCCGC
GAACCCGAGATCCCGCTGCTGTCCACCGTCACCGGTCGGCCCGCCACCCGGCTCGACGCC
GAGCACTGGTACCGCAACCTGCGCGAGCCCGTCCGGTTCGCCGACGCCGTCACCGCCCTG
CTCGACCGGGGGCACCGGGTGTTCGTGGAGGTGAGCCCGCACCCGGTGCTCACCACCAGC
GTCGTCGACCTCGCCGCACCGCACCGCACCGCCGTCGTGGGCACGCTCCGCCGCGACGAG
GGCGGGCTCGACCGGTTCCTGCTGTCCGCGGGTGAGCTGCACGTGCGCGGCGTGCCCGTC
GACCTCGCCCGGCACGCGGGCGCGGGCACGGCCGAGGTGCCGACCACGGTCTTCCAGCGC
GAGCGGTACTGGCTGGACGCGCCCCGCCCGTCCGGCGACGCGCGCGCCGCCGGGCTCGTG
CCCTCGGGGCACCCGCTGCTGGGCGCGCTCGTGCCGCTCGCGGACGGCGCCGTCCTCACC
GGGCGGCTCTCGCTGTCGGCCCAGCCGTGGCTCGCCGACCACCGGGTGCTCGGCGCGGTC
GTCGTGCCCGGTGTCGCGCTGGTGGAGCTGGCGGTGCGCGCGGGCGACGAGGTCGGCTGC
CCGGCGCTGGAGGAGCTGGTGGTGGCCGCGCCGCTGGTGGTCCCGGAGAGCGGTGACGTG
GCGGTGCGCGTGGTCGTCGGCCCGCGCGGCGCGGACGGGACGCGCCCCGTGGACGTGCTC
TCCGGCGACCAGCCCCCGGTGCTGCACGCGCGCGGCGTCCTCGGCCCGCCCCGCGCGCCC
GAGCCGGTCGGGGGCCAGTGGCCGCCCGCGGGCGCCGAACCGGTCGACCTCGCCGGGCTC
TACCCGGACTTCGCCGCCAAGGGCCTGGAGTACGGGCCGCACTTCCAGCGGCTGACCCGG
CTGTGGCGGCGCGGCGACGAGGTGTTCGGCGAGGTCGAGCTGACCGGCGAGCAGCGCAGG
GCCGCCGGGTTCGCGCTGCACCCGGCGCTGCTGGAGTGCGCGCTGCACGTGAGCAGCTTC
ACCGGGCAGCGGGTCGCGGGGGACGGCGGCGCGGTGGGGCTGCCGTTCTCCTTCTCCCGC
GTCGCGCTGCACCGCTCCGGCGTGGACGCGGTGCGGGTGCGGGCGGCGCGGGTCGGGGAC
GGGGTCGTCTCGCTCGACCTCGCCGACGGCGCCGGCCGCCCGGTGGCGCGGATCGGCGAG
CTGGCCTTCCGACGGGCGGGCGGGATCCGGTCCGGTCCACCGGGGGTGCTGCTGGAGACC
GCGTGGCGCCCGGTGGAGCTGCCCGAGGCCCCACCGCCCGCCGACGTGACGACCGTGGTG
GGGGACTCGGTCGAGCGGGTGCTCGCCGCCCTGCGCGAGACGCTCGCCGGGGACGGGCGG
CTGCTCGTGCTCACCGGCGACCCCGGCGCGCCGATCGGCACGGCGGTCGGCGGGGCGGTG
TGGGGGCTGGTGCGCTCTGCCCAGCTCGAGGAGCCCGGCCGGATCGTCCTGGCCGACCTG
GACACCGCGCCCGAGCGGGCGGGCGCGGTGCTGGACCGGGTGCTGGCCTCCGGTGAGCCG
CAGGTCGCGATCCGCGGCGGGGCCGTGCTCGTGCCCCGGTTGACGCCGGTCCCGGTGGTC
GCCGGGCCCGCCGGGCTGGACCCGGCGAAGACGGTGGTGCTCACCGGCGGGACCGGCGCG
CTCGGCAGGCTCGTCGCGCGCCACCTGGTCACCGAGCACGGCGCCCGCAGGCTCCTGCTG
CTCGGGCGCGGCGGCCCGGAGCGGGCGCGCGACCTGGTCGCCGAGCTGACCGGCCTGGGC
GCCGAGGTGGAGGCCCTCGCCTGCGACGTCGCCGACCGGGGCGCGCTGTCGACCGCGCTC
GCCGGGCGCGCCCTCACCGCCGTCGTGCACCTGGCGGGCGCGCTGGACGACGGCGTCGTG
ACCGCGCTGACCCCCGAGCGCCTGGCCGCCGCGTTCGCGCCCAAGGCGCTGGCCGCCCGG
CACCTGGACGAGCTGACCAGGGACCAGGACCTGGCCGCGTTCGTGCTGTTCTCCTCCGTC
GCCTCGGTGCTCGGCTCGGCCGGACAGGCCAACTACGCGGCGGCCAACGGGTTCCTGGAC
GGCCTCGCGCACGAGCGGCACGCGCTCGGCCTGCCCGCCACGTCCATCGCCTGGGGGCTG
TGGGACGTGGACAGCGCGCTGGTGGGCGAGCGCGGCGCGGCCGACCGGGAGCGGCTGGCC
GCGTCCGGGATGCGCCCGCTGCCCGCGCCCGCCGCGCTCGACCTGCTCGACGCCGCGCTG
GCCTGCGGGCGGCCCGCCGTCACCGCGCTGGCGCTGGCCCGCGCCGCCGACCGGGCGCCC
GCCGCGCTGGGCGAGGTGGTCGCGTCGCGCCGGGCGCCCGCGCCCGCCGCGCCGGTACCA
GGGGGCCCGGCCGCGCTGCTCGCGCTGGTGCGGGCCGAGGCCGCCGCCGTGCTCGCCCTG
CCCGTCGCGGGCGTCGACGAGCGGCGGGCGTTCCGCGACCTCGGGGTCGACTCGCTGACC
GGGGTGGAGCTGCGCAACCGGCTCGCCGAGGCCACCGGGCTGCGGCTGCCCGCGACGCTG
GTGTTCGACCACCCGACCCCGGTCGAGCTGGCCGCCGCGCTGCTGGAGCGGGTGGGCGGG
ACCTCGGGACCGGCGGGCGGCGCGTCCGCCCCCGCGCCCGCGTCCGCCCCCGGCGAGCCG
ATCGCGATCGTCGGCATGGCCTGCCGCGCGCCCGGCGGGGTCGCCTCGCCCGAGGACCTG
TGGGACCTGGTCGCGGGCGGCGTCGACGCGGTCGGCCCGTTCCCCGCCGACCGGGGCTGG
GACCTGGAGCGGCTGGTGCACCCCGACCCCGACCACGAGGGCTCCACCTACGTCGACCGG
GCCGCGTTCCTGGACGACCCGGCCGGGTTCGACGCGGACTTCTTCGGCATCTCCCCGCGC
GAGGCCCTCGCGATGGACCCGCAGCAGCGGCTGCTGCTCGAAGTCGCGTGGGAGGCGTTC
GAGCGCGCGGGCATCGCCCCGGACTCGGTGCGGGGGACGGACGTCGGCGTGTTCGCGGGC
GTCACCAACCACGACTACGACCAGGTGCTCCCGCGCGGCGACGACGGCTACCGGATCACC
GGCGTGTCGGGCAGCGTCGTGTCGGGGCGCATCGCCTACGCGCTCGGGCTGGAGGGCCCC
GCGCTGTCGGTGGACACCGCCTGCTCGGCCTCGCTGGTGGCGATCCACCTCGCGGTGCGG
GCCCTGCGCGGCGGCGAGTGCTCGATGGCGCTGGCGGGCGGCGCGACGGTCATGGCCACG
CCCAGGGGGTTCGTGGAGTTCGCCAGGCAGCGCGGGCTCGCCCCGGACGGCCGCTGCAAG
CCGTTCTCGGCGTCGGCGGACGGGACGGCGTGGTCCGAGGGTGCGGGGTTGGTGCTGCTG
GAGCGGCTGTCGGACGCGCGGCGCAACGGGCGTCGGGTGCTGGCCGTGGTGCGGGGCAGC
GCGGTGAACTCGGACGGGGCGTCGAACGGGTTGACGGCGCCGAGCGGTGCGGCGCAGCAG
CGGGTGATCCGGGCGGCGCTCGCGGACGCCGGTCTGTCCACTTCGGACGTGGACGCGGTG
GAGGCGCACGGGACCGGGACCCGGTTGGGCGACCCGATCGAGGCCCGCGCGCTGGTCGCC
ACCTACGGGCGGGACCGGGACGGCGAGCCGCTGTGGCTGGGGTCCCTCAAGTCCAACCTC
GGGCACATGCAGGGCGCCGCCGGGATCGGCGGTGTGGTGAAGACCGTGCAGGCGCTGCGG
CACGGGGTGCTGCCGCGCACCCTGCACGTGGACGCGCCCACCCCCGAGGTCGAGTGGGAC
GGTGTCGCGCTGCTGGCCGAGCAGCGCGCGTGGCCCGAGGTGGACCGGCCGCGCCGCGCG
GCGGTCTCCTCGTTCGGGGTCAGCGGCACCAACGCGCACGTCGTGCTGGAGCAGGCCCCC
GCCGAGCGGACCGCAGCCGAAAACCCCGGTGGGAACGCAGCCGGGCGCGTTGGGGACTTT
CCCGGCGTGCTGCCGCTGGTGCTGTCCGCGCGGAGCCCGGCGGCCCTGCGCGCCCAGGCC
GCGCGGCTCGCCGACGTCCTCGACGACCTGCCGCTCCGGGACGTCGCCTCCACGCTCGCG
CGGGGGCGCGCCGCGCACGAGCACCGCGCCGTCCTCGTCGCCGCCGACCGGGACGCCCTG
CGCGCCGGGCTGCGCGCGCTGGCCGGGGGACGTCCGCACGCCGCCGCCCGCACCGGGGTG
CGCGCGCCCGGCGGGCTGGCGTTCGCCTTCTCCGGGCAGGGCAGCCAGTTCGTCGGCATG
GGGCGCGGCCTGCACGCCGCGTTCCCCGCGTTCGCCGAGGTGTTCGACCGGGTGTGCGCG
GCGCTGGACGAGCGGCTGGGCGAGCTCACCGGGCGCTCCGTCCGGGACGTCGTGCTCGAC
GGGCCCGCCGAGCTGCTGGCGGAGACCGTCCACGCCCAGCCCGCGCTGTTCGCCCTGGAG
GTCGCGCTGGCGCGGCTGCTGGAGGGGTGGGGCGTCGTGCCCGACGTCGTGCTCGGGCAC
TCGGTCGGCGAGCTGGCCGCCGCGCACGTCGCGGGCGCGCTGTCGGAGGCGGACGCCTGC
GCGCTCGTCGCGGCCAGGGCGCGGGCCATGCACGGGGCCCGGCGCGGCGGGGCGATGCTC
GCGGTGTCCGCCACCGAGGAGCAGGTCGCGCCGCTGCTGGGCGGGGGCTTGGCGCTGGCC
GCCGTCAACGGGCCGGGCGCGGTCGTGCTGTCCGGTGACGGGGACGCGGTCGAGCGGGCC
GCCGCCGAGTGCGCCGGGCGCGGGTGGCGGACCAGGGCGCTGCGGGTGAGCCACGCCTTC
CACTCCGCGCACCTGGACGGGGTGCTCGGTGAGCTGGCCGTGGCCGCGCGGCGGGTCGAG
GTCCGGCCCCCGCGCGTCCCGCTGCTGTCCACGCTCACCGGCGCGCCCGCCGACGTCGAG
CTGCTCGCCGACCCGGACTACTGGGCTCGGCAGGCCAGGGAGGCCGTCCGGTTCGGCGAC
GGCGTCGCCCGGCTGGGCGAGCTGGGCGTGACGCGGGTGCTGGAGGTGGGGCCCGGCGGC
GCGCTGGCCGCCCTGGTGCGCGAGCAGGTCGCGCCGGGGGCCGCGGTGGCGCGCGGGCTG
GTCGACGGGGACGAGGTGGTCGGGCTGCTCTCCGGGATCGGCGACCTGTTCGCGTCCGGG
GCCCCGGTCGACTGGACCGCGCTGCTGGGCGGGCCGGGGGCGGTCGTCGACCTGCCCACC
TACCCGTTCCAGCGCACCAGGTTCTGGCCCGCGGCGCAGGCGCCCGGCGCGTGGTGCGAG
GTCCGCCTGGAGCGGTTGCCCGCGCCTCGGGGCGGCGGGTCGGCGACCGTGCTGGAGGTG
GGGCCGACCGCCTCGGCCGAGGAGCTGGCGAGCGTCGCCGACGTGCTGGCCGAGCACAGC
GGCCCGCTGCTGGTGGTCACCGGGGACGGCGCGGTGGCCGGGCTGGTGCGCTCGCTGCCG
GACCGCGCGGACGTCGTGCTGGCGGACGTGGACGACGACCCGGCGTCGGTGGCGCTGCTG
CCCGCGCTGCACGGCTGCGGCGAGCCCGAGCTGGTGGTGCGCGCGGGCGAGGTGCTCGCG
CCCAGGCTGCGGCGCGTCACCGCGACCGGCGCCGCCCCCTCGGGGCCGGTGCTGGTGGTC
GGGGAGGCCGCGCGGGGCGTGGTGGCGGCCTGCGGGGTGCGCGAACCGGTCGTGCTGCCC
GCCGACTCGGGCCGGGCGGAGCTGGTGGCCGCGCTGGACGACCACCGCCCGGACTGGGTC
GTGCTCGCCGTCGCGCCGGACGACGCGGGCCTGGCGCTGGCCGCGCTGCTGGACGAGCTG
ACCCGCGACCGGCCGCCCGCGCTGTTCGCGCTGTGCGGACCGGCGGGCGCGCTCGGCGAC
GCGGCGCGCCCCGAGGACGCGGTGGCCGCCGGGGTGCTGGCCGCGCTCGTGCGCCGCCGC
CGCGGGGACGGCCTGCCCGCGACCCTGCTGTGCGCGGGACCGGGCACGCCGCCGCTGAGC
CCCGAGCTGGGGGTGGTCGTGTTCGCGCGGACGCCCGAGGTGGTGCTGCCCGAGCCGAGG
ACCGCCCAGCCCGAGCCGGACGCGGTCGACCTGACCGGCCCGGACCGGGACCGGGTGCTG
CTGGCGCTGGTGCGCGACCACGCCGCCGCCGTGCTGGGCCGCGCGGACGGCCCGGTGGAC
GGGCGGGCCGCGTTCCGGGACCTGGGGTTCGACTCGATGACGGCGGTGGAGCTGCGCGAC
CGCCTGGCCGCCGCCACCGGCCTGACCCTGCCCGCGACCCTGCTGTTCGACCACCCGACG
GCGGCCGAGGTCGCCGCCGTGCTGGCGGGGCTGCTCGACGGCGGCGACCCGGTGACCTCG
GGCCTGGACCGCCTGGAGGCCGCGCTCGCCGACCCGCCCGCCGACCCCGCGCTGCGCGCG
TCCGCCGCCGCCAGGCTGCGCGCCCTGCTCGCCGCCCTGTCCGACGAGGGCGACGGCCGG
GACGGCGCTGGCCAGGACGGCGCTGCCGCGCTCCGGGACGCGGGCGTGGCGGAGCTGTTC
GAGTTCATCGACACCCGGCTGGGCCGCGCCGAGCGGGCCTGA
[3] KS29..404
[3] AT646..959
[3] methoxymalonyl-ACP827..840
[3] DH1004..1158
[3] KR1410..1584
[3] ACP1663..1735
[4] KS1759..2131
[4] AT2288..2571
[4] malonyl-CoA2478..2482
[4] ACP2916..2989
[3] KS85..1212
[3] AT1936..2877
[3] methoxymalonyl-ACP2479..2520
[3] DH3010..3474
[3] KR4228..4752
[3] ACP4987..5205
[4] KS5275..6393
[4] AT6862..7713
[4] malonyl-CoA7432..7446
[4] ACP8746..8967

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001190 Speract/scavenger receptor (Domain)
[632-677] PS50287
PS50287   SRCR_2
IPR001227 Acyl transferase domain (Domain)
[640-766] G3DSA:3.40.366.10 [836-947] G3DSA:3.40.366.10 [2286-2410] G3DSA:3.40.366.10 [2477-2571] G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1410-1571] 5.69999999999998e-52 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
[1693-1708] PS00012 [2947-2962] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1670-1734] 9.10000000000001e-12 PF00550 [2924-2987] 7e-12 PF00550
PF00550   PP-binding
[1665-1740] 5.59999999999999e-41 G3DSA:1.10.1200.10 [2920-2992] 5.59999999999999e-41 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1658-1752] 1.70000295590054e-21 SSF47336 [2911-2996] 1.89999859865865e-20 SSF47336
SSF47336   ACP_like
[1663-1735] PS50075 [2916-2989] PS50075
PS50075   ACP_DOMAIN
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[29-279] 7.5e-89 PF00109 [1759-2006] 7.20000000000003e-94 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[287-404] 4.30000000000003e-46 PF02801 [2014-2131] 5.59999999999999e-46 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[646-959] 2.39999999999997e-89 PF00698 [2288-2571] 6.09999999999996e-60 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[644-943] 9.19998414420354e-59 SSF52151 [2285-2590] 4.89996723796399e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[769-835] 2.59999946718995e-15 SSF55048 [2415-2477] 3.30000526807186e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[31-291] G3DSA:3.40.47.10 [292-428] 8.60000000000005e-124 G3DSA:3.40.47.10 [473-496] 8.60000000000005e-124 G3DSA:3.40.47.10 [1761-2018] G3DSA:3.40.47.10 [2019-2182] 8.60000000000005e-124 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[21-403] 7.60000011115808e-99 SSF53901 [1751-2181] 2.0000139277205e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1410-1585] 1.99999999999999e-31 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[192-208] PS00606 [1919-1935] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[648-940] 2.19999900980707e-98 SM00827 [2289-2592] 3.10001307370203e-100 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1667-1738] 4.40000933643891e-31 SM00823 [2921-2992] 6.30000946287306e-29 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[1004-1158] 2.59998233910302e-70 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[31-497] SM00825 [1761-2181] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1410-1584] 6.70002630158883e-53 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Ansam_00270 Ansam_00270   Ansam_00290 Ansam_00290
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