Ansam_00410 : CDS information

Location

Organism	Actinosynnema pretiosum subsp. auranticum
Strain	ATCC 31565
Entry name	Ansamitocin
Contig	AF453501
Start / Stop / Direction	82,745 / 81,069 / - [in whole cluster] 2 / 1,678 / + [in contig]
Location	complement(81069..82745) [in whole cluster] 2..1678 [in contig]
Type	CDS
Length	1,677 bp (558 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein(fragment)
Product (GenBank)	unknown
Gene
Gene (GenBank)	asm37
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q8KUI7 PmId [12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002) comment Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。 <cluster I> Asm37(558aa, partial) : Unknown 2つのcluster間にある30kbp領域（asm37 and asm38の一部を含む）を削除したmutant HGF057は、識別可能な表現型の変化なし。

Sequence

>hypothetical protein(fragment) [unknown]
MVPGLVVALAATGVLAAPASAAITSPTVTSTATTVTFEVGRTGTPAFSRVYVDTDRSAAT
GYAQGGIGADHLLENGSLYRHSGGGWAWTQVRTVPFTSASGVARWTVERADLGEQASPND
VDVVFQVEAPVETSAKVTHTYTAGAGGSVAYQPSSANVPNPERGLYHHTGDCDKADLSAD
ALRRYRTEQGITLAMCVFYLAEHRSSPIAQTALDRLQRQFDAARVAGVKLVLRFAYTASE
SGEDAPKERVLAHIDQLAPLLRANADVTAVVQAGFVGAWGEWYYTKNFGNAGVVSATDQA
NRKAVVDKLLTALPADRAVQLRTPKFKRALYGGGSTRIGHHNDCFLASDTDFGTYEDPAT
EYPYLEAETRTLPMGGETCAVNPPRTDCPTATAELSRFHWTYLNTDYQQDVLRAWSAQGC
LAEVTAKLGYRFTLRDSVFPTTTTRGAALPVRVNLTNDGYAAAVNQRTVNLVLRNTTTGA
LTRLPLTADPRTWLPGARAIAQDAQIPPSLATGQYALLLELADPALPGRPEYSIQTANTG
LWDARTGLNDLKQTITVS

>hypothetical protein(fragment) [unknown]
ATCGTGCCGGGTCTGGTGGTGGCCCTCGCCGCCACCGGCGTCCTCGCGGCCCCCGCGAGC
GCCGCCATCACCAGCCCCACCGTGACCAGCACCGCCACCACCGTCACCTTCGAGGTCGGC
CGCACCGGGACGCCCGCGTTCTCGCGCGTCTACGTCGACACCGACCGCAGCGCGGCCACC
GGCTACGCGCAGGGCGGGATCGGGGCGGACCACCTGCTGGAGAACGGTTCCCTCTACCGC
CACAGCGGCGGCGGCTGGGCGTGGACGCAGGTGCGGACCGTGCCGTTCACCTCCGCCTCG
GGCGTCGCGCGGTGGACCGTCGAGCGGGCCGACCTCGGCGAGCAGGCGAGCCCGAACGAC
GTCGACGTGGTGTTCCAGGTCGAGGCCCCGGTGGAGACCTCGGCGAAGGTCACCCACACC
TACACCGCGGGCGCGGGCGGCTCGGTCGCCTACCAGCCGTCGAGCGCGAACGTCCCCAAC
CCGGAACGCGGTCTTTACCACCACACCGGCGACTGCGACAAGGCCGACCTGTCCGCCGAC
GCGCTGCGCCGGTACCGCACCGAGCAGGGCATCACCCTCGCGATGTGCGTGTTCTACCTG
GCCGAGCACCGCTCGTCCCCGATCGCCCAGACCGCCCTCGACCGCCTGCAGCGCCAGTTC
GACGCCGCGCGGGTCGCGGGCGTGAAGCTGGTGCTGCGCTTCGCCTACACCGCGTCGGAG
TCCGGCGAGGACGCGCCGAAGGAGCGGGTCCTGGCGCACATCGACCAGCTCGCGCCGCTG
CTGCGCGCGAACGCGGACGTGACCGCCGTGGTGCAGGCCGGGTTCGTCGGCGCGTGGGGC
GAGTGGTACTACACCAAGAACTTCGGCAACGCGGGCGTCGTCTCCGCGACCGACCAGGCC
AACCGCAAGGCGGTGGTGGACAAGCTCCTGACCGCGCTCCCGGCGGACCGGGCGGTGCAG
CTGCGCACGCCGAAGTTCAAGCGCGCCCTGTACGGGGGCGGCAGCACCAGGATCGGCCAC
CACAACGACTGCTTCCTGGCCAGCGACACGGACTTCGGCACCTACGAGGACCCGGCGACC
GAATACCCGTACCTGGAGGCGGAGACGCGGACGCTGCCGATGGGCGGCGAGACCTGCGCG
GTGAACCCGCCCCGCACCGACTGCCCGACCGCGACCGCCGAGCTGTCCCGCTTCCACTGG
ACGTACCTGAACACCGACTACCAGCAGGACGTGCTGCGCGCGTGGTCGGCGCAGGGCTGC
CTGGCGGAGGTCACGGCCAAGCTCGGCTACCGCTTCACCCTGCGCGACTCGGTGTTCCCC
ACGACGACCACCAGGGGCGCCGCGCTGCCCGTGCGGGTGAACCTGACCAACGACGGCTAC
GCCGCGGCGGTGAACCAGCGCACCGTGAACCTGGTGCTGCGCAACACCACCACCGGCGCC
CTGACCCGCCTCCCGCTCACCGCGGACCCCCGGACCTGGCTGCCGGGCGCCCGCGCCATC
GCGCAGGACGCCCAGATCCCGCCGTCGCTCGCGACGGGGCAGTACGCGCTCCTGCTGGAA
CTGGCCGACCCCGCCCTGCCGGGCCGCCCGGAGTACTCGATCCAGACGGCGAACACCGGC
CTGTGGGACGCGAGGACGGGGCTGAACGACCTGAAGCAGACCATCACGGTGTCGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Ansam_00400 Ansam_00420