Ansam_00510 Ansam_00510   last last

Ansam_00520 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction94,650 / 93,097 / - [in whole cluster]
11,904 / 10,351 / - [in contig]
Locationcomplement(93097..94650) [in whole cluster]
complement(10351..11904) [in contig]
TypeCDS
Length1,554 bp (517 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator(fragment)
Product (GenBank)transcription activator
Geneasm48
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF11; member of LuxR family
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

cluster II を削除するとansamitocinsの産生がなくなるが、AHBAを補充すると産生は回復するので、cluster II はAHBA形成のみに必要な遺伝子を含む。

<cluster II>
Asm48(518aa, partial) : Transcriptional activator(Function of homologue)

本文にコメントなし。
Related Reference
ACC
Q06BL5
NITE
Gelda_00100
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
comment
Blast 5th, id56%, 1e-136
Streptomyces hygroscopicus_gdmRII
GdmRII

gdmRI, gdmRIIのいずれか一方の欠損株ではgeldanamycinの生産が完全に消失し、欠損した遺伝子をプラスミドで相補すると geldanamycin生産が回復すること、二つの遺伝子の転写は独立に行われ互いを制御していないこと、二つの遺伝子の転写はpolyketide生合成に関与する遺伝子と協調し、post-PKS modification 遺伝子である gdmNとは協調していないことが示されており、gdmRI と gdmRIIはgeldanamycin 生合成経路におけるpolyketide 生合成遺伝子特異的なpositive regulatorsであると結論付けている。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator(fragment) [transcription activator]
CSPDRVRRAAVQARLAAAEWLVNPSTSARHHTALLAAFRAGRLSVRDSATLVEHLRWAGS
TADSDAVLAGLRADPRAAEEVPVLEHWLAGAYPGAARPGTAPGRDVDSARTRAEQAPRAN
AVLLDVLVAGDGDHVADRAEAVLREVRLARDPGGSGGAAVPALSALLYSDRADVAASWCE
QLLSARAVPLLPVPRAQVLALAAESALRRGDHPRADELAREALTVLPPTAWGVSVGLPLS
TRILALTRMGRCDEAAAVVARPVPNGVFGHRTSVDYLHARGHFLLARGRPRAALGDFLLC
GEQLTRWGLGGGCAPVPWRTAAAEAWLAQGDPDRARALAHEQLGRPGSDSRRARGQALRL
LAATGPVKRRPRLLREAVAVFEAVDDKYELARTLRDLGGAQRALGEHALARRVVRRGWHV
ARMCGAAPLCEELMPTADGLVPAQQASAACGSDTGRLTSSEHRVAALAASGLTNREIAVK
LYVTHSTVEQHLTRVFRKLGIKQREQLPPALSADRSG
selected fasta
>putative LuxR family transcriptional regulator(fragment) [transcription activator]
TGCAGCCCGGACCGGGTGCGCAGGGCGGCCGTGCAGGCGCGGCTGGCGGCGGCCGAGTGG
CTGGTGAACCCGTCGACCTCGGCCAGGCACCACACCGCGCTGCTGGCGGCGTTCCGCGCG
GGCAGGTTGTCGGTGCGCGACAGCGCGACGCTGGTGGAGCACCTGCGCTGGGCCGGGAGC
ACCGCCGACTCGGACGCGGTGCTCGCCGGGCTGCGGGCCGACCCGCGCGCCGCCGAGGAG
GTGCCGGTGCTGGAGCACTGGCTGGCTGGCGCCTACCCCGGTGCGGCCAGGCCGGGGACC
GCGCCGGGGCGGGACGTGGACTCCGCGCGCACCAGGGCGGAGCAGGCGCCGAGGGCGAAC
GCGGTGCTGCTGGACGTGCTGGTGGCCGGGGACGGCGACCACGTGGCCGACCGGGCGGAG
GCGGTGCTGCGGGAGGTGCGGCTGGCGCGGGACCCCGGCGGCTCCGGCGGTGCGGCCGTG
CCTGCGCTGTCCGCGCTGCTGTACTCGGACCGCGCGGACGTGGCCGCCTCCTGGTGCGAG
CAGCTGCTGTCGGCGCGGGCCGTGCCGCTGCTGCCGGTACCGCGCGCGCAGGTGCTGGCG
CTGGCGGCCGAGTCGGCGCTGCGCCGGGGCGACCACCCGAGGGCGGACGAGCTGGCGCGG
GAGGCGCTGACCGTGCTGCCCCCCACCGCGTGGGGGGTGTCGGTGGGGCTGCCGCTGAGC
ACCCGGATCCTGGCGCTGACCAGGATGGGCCGCTGCGACGAGGCGGCGGCCGTGGTGGCG
CGGCCGGTGCCGAACGGGGTGTTCGGGCACCGCACCAGCGTGGACTACCTGCACGCGCGC
GGGCACTTCCTCCTGGCGCGGGGACGGCCGCGCGCGGCGCTGGGCGACTTCCTGCTGTGC
GGGGAGCAGCTGACCCGGTGGGGTCTGGGCGGCGGGTGCGCGCCGGTGCCGTGGCGGACC
GCGGCGGCGGAGGCGTGGCTGGCGCAGGGCGACCCGGACCGGGCGCGGGCGCTGGCGCAC
GAGCAGCTCGGCAGGCCGGGCTCGGACAGCAGGCGGGCGCGCGGGCAGGCGCTGCGGCTG
CTCGCGGCGACCGGCCCGGTGAAGCGGCGCCCGCGGCTGCTGCGGGAGGCGGTGGCGGTG
TTCGAGGCCGTGGACGACAAGTACGAGCTGGCGCGGACCCTGCGCGACCTGGGAGGGGCG
CAGCGGGCGCTGGGCGAGCACGCGCTGGCGCGCCGGGTGGTCCGGCGCGGGTGGCACGTC
GCCCGGATGTGCGGGGCGGCGCCGCTGTGCGAGGAGCTGATGCCCACCGCCGACGGGCTG
GTGCCCGCGCAGCAGGCGTCGGCGGCCTGCGGGTCGGACACGGGCCGGTTGACCAGCTCG
GAGCACCGGGTGGCCGCGCTCGCGGCGTCGGGGCTGACGAACCGGGAGATCGCGGTGAAG
CTGTACGTCACGCACAGCACGGTGGAGCAGCACCTGACGCGGGTGTTCCGCAAGCTCGGG
ATCAAGCAGCGGGAGCAGCTGCCGCCCGCGCTGAGCGCCGACCGGTCGGGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[457-471] 1.20000117458134e-09 PR00038 [471-487] 1.20000117458134e-09 PR00038 [487-499] 1.20000117458134e-09 PR00038
PR00038   HTHLUXR
[471-498] PS00622
PS00622   HTH_LUXR_1
[454-511] 1.20000117458134e-20 SM00421
SM00421   HTH_LUXR
[450-517] PS50043
PS50043   HTH_LUXR_2
[456-507] 2.2e-11 PF00196
PF00196   GerE
IPR011990 Tetratricopeptide-like helical (Domain)
[195-251] 1.1e-16 G3DSA:1.25.40.10 [252-465] 1.1e-16 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[466-507] 1.2e-17 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[435-507] 2.70000183580794e-14 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Ansam_00510 Ansam_00510   last last
Page top