Rever_00010 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	909 / 1 / - [in whole cluster] 909 / 1 / - [in contig]
Location	complement(1..909) [in whole cluster] complement(1..909) [in contig]
Type	CDS
Length	909 bp (302 aa)

Annotation

Category
Product
Product (GenBank)	putative amidohydrolase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf-4
Reference	ACC G1UDT1 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative amidohydrolase]
MLVIDTHTHVISPDEDRYPTDPIGGHRSTWSQDHPVDLDGLLHALDDAGIDRAVVVQAST
VYGHDNRYLADSVAAHPDRLTGVYSLDATAPDAVDRINHWQSRGLNGFRLFTTGTTMPGQ
ADWLGHPDSYPAWEHAEKHDIPICLQMTIHGIPALRTTLDHYPRTRVLLDHCARPDLTDG
PPYHTSQALLDLAQYPGVHLKLTHRALDAATQGASTIPDFLTTLHTTYGPSRIMWGSNYP
AAQGPLTNLLTQARTHLTTLPETATTAILGGTANTFYFHTPATPATPTPATPATATPGAH
HA

>[putative amidohydrolase]
ATGCTGGTGATCGACACCCACACCCACGTCATCTCGCCCGACGAGGACCGCTACCCCACC
GACCCGATCGGCGGACACCGCTCCACCTGGTCACAAGACCACCCCGTCGACCTCGACGGA
CTCCTGCACGCCCTCGACGACGCCGGCATCGACCGCGCCGTCGTCGTCCAAGCCTCCACC
GTCTACGGACACGACAACCGCTACCTCGCCGACTCCGTCGCCGCCCACCCCGACCGCCTC
ACCGGCGTCTACTCCCTCGACGCCACCGCACCCGACGCCGTCGACCGCATCAACCACTGG
CAATCCCGCGGCCTCAACGGCTTCCGCCTGTTCACCACCGGCACCACCATGCCCGGACAA
GCCGACTGGCTCGGCCACCCCGACAGCTACCCCGCCTGGGAACACGCCGAAAAACACGAC
ATCCCCATCTGCCTCCAGATGACCATCCACGGCATCCCCGCCCTACGCACCACCCTCGAC
CACTACCCCCGCACCCGCGTCCTCCTCGACCACTGCGCCCGCCCCGACCTCACCGACGGA
CCCCCCTACCACACCTCCCAAGCCCTCCTCGACCTCGCCCAATACCCCGGCGTCCACCTC
AAACTCACCCACCGCGCCCTCGACGCCGCCACCCAAGGCGCCTCCACCATCCCCGACTTC
CTCACCACCCTCCACACCACCTACGGACCCTCCCGCATCATGTGGGGATCCAACTACCCC
GCCGCCCAAGGCCCCCTCACCAACCTCCTCACCCAAGCCCGCACCCACCTCACCACCCTC
CCCGAAACCGCCACCACCGCCATCCTCGGCGGCACCGCCAACACCTTCTACTTCCACACC
CCCGCAACCCCCGCAACCCCCACCCCGGCAACCCCCGCAACCGCCACCCCCGGGGCCCAC
CATGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006992 Amidohydrolase 2 (Family) PF04909 Amidohydro_2
SignalP	No significant hit
TMHMM	No significant hit

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