Mith_00050 Mith_00050   Mith_00070 Mith_00070

Mith_00060 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction8,142 / 9,209 / + [in whole cluster]
8,142 / 9,209 / + [in contig]
Location8142..9209 [in whole cluster]
8142..9209 [in contig]
TypeCDS
Length1,068 bp (355 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglucose-1-phosphate thymidylyltransferase
dTDP-glucose synthase
Product (GenBank)dTDP-glucose synthase
Gene
Gene (GenBank)mtmD
EC number2.7.7.24
Keyword
  • D-olivose
  • D-oliose
  • D-mycarose
Note
Note (GenBank)
Reference
ACC
PmId
[9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997)
comment
streptomycinを産生するStreptomyces griseusのstrD and strEをプローブとして、mithramycinの糖部分の生合成に関する遺伝子(mtmD, mtmE)をmithramycin産生株S. argillaceusのcosAR7から分離。

MtmDは抗生物質生合成に関連したstreptomycetesのputative glucose-1-phosphate:TTP thymidylyl transferases(StrD, GraD, SnoD, DnrL)に似ている。

mtmD and mtmEをE.coliで一緒に発現し、その細胞抽出液を用いてdTDP-D-glucose synthase活性、dTDP-D-glucose-4,6-dehydratase活性を測定。IPTGでの遺伝子発現誘導で両酵素活性は3-4倍に増えた。

mtmDを置換して不活化したmutantは、糖を持たない4環のpremithramycinoneを蓄積した。よって、いくつか（またはすべて）のglycosylationsは4th ringの切断の前に起こると示唆される。

close this sectionSequence

selected fasta
>glucose-1-phosphate thymidylyltransferase [dTDP-glucose synthase]
MKALVLSGGSGTRLRPITHTSPKQLVPVANKPVLFYALEDIAQAGITDVGIVVGETAREI
EDAVGDGSKFGLQVTYLPQEKPLGLAHAVLIARDYLGDDDFVMYLGDNFVVGGIAGLVDE
FRADRPDARILLTPVPDPTAFGIAELDGEGRVVRLEEKPTHPKSDMAVIGVYLFTPLVHE
AVRAIEPSWRGELEITHAIQWLIDNRHDVRSTVIHGYWKDTGNVMDMLEVNRTVLEGVEP
CTEGPVDAESEVIGRVRIEAGARVSNSRIVGPAVIGSGTVISGSYIGPYTSVSEGCRIED
SEIEFSIVLRDSRVRGVQRVRGSLIGRSVTVAPAPRKPAGTRLVLGDHSEGQISS
selected fasta
>glucose-1-phosphate thymidylyltransferase [dTDP-glucose synthase]
ATGAAGGCACTCGTTCTGTCCGGTGGCTCGGGCACACGCCTGAGGCCCATCACCCACACA
TCGCCCAAGCAGCTGGTGCCGGTGGCGAACAAACCTGTTCTCTTCTACGCCCTGGAGGAC
ATCGCGCAGGCCGGGATCACCGACGTCGGGATCGTCGTCGGTGAGACGGCCCGCGAGATC
GAGGACGCGGTGGGCGACGGTTCCAAGTTCGGCCTCCAGGTGACCTACCTGCCGCAGGAG
AAACCCCTGGGACTCGCCCACGCGGTCCTGATCGCCCGCGACTACCTCGGTGACGACGAC
TTCGTCATGTACCTGGGAGACAACTTCGTGGTCGGCGGCATCGCCGGTCTGGTGGACGAG
TTCCGTGCGGACCGGCCCGACGCGCGGATCCTGCTGACCCCCGTTCCCGATCCGACGGCC
TTCGGCATCGCCGAGCTGGACGGGGAGGGCCGGGTCGTACGCCTGGAGGAGAAGCCGACG
CACCCCAAGAGCGACATGGCGGTGATCGGCGTCTACCTGTTCACCCCGCTCGTGCACGAG
GCCGTGCGCGCCATCGAGCCGTCGTGGCGGGGGGAGCTGGAAATCACCCATGCCATCCAG
TGGCTCATCGACAACCGCCACGACGTGCGGTCTACCGTGATCCACGGCTACTGGAAGGAC
ACCGGCAACGTCATGGACATGCTGGAGGTCAACCGGACGGTGCTGGAGGGCGTCGAGCCG
TGCACCGAGGGCCCGGTCGACGCGGAGAGCGAGGTGATCGGCCGCGTCAGGATCGAGGCC
GGCGCCCGGGTCAGCAATTCGCGGATCGTCGGTCCCGCGGTGATCGGGTCGGGCACGGTG
ATCAGCGGTTCCTACATCGGGCCGTACACGTCCGTCTCCGAGGGCTGCCGGATCGAGGAC
AGCGAGATCGAGTTCTCGATCGTGCTGCGCGACTCACGGGTGCGGGGCGTCCAGCGGGTC
AGGGGGTCCCTCATCGGCCGCAGTGTCACCGTGGCCCCCGCCCCCCGCAAGCCGGCCGGC
ACCCGGCTCGTTCTCGGCGACCACAGCGAGGGGCAGATCTCCTCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-236] 2.50000000000001e-59 PF00483
PF00483   NTP_transferase
IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family)
[2-350] TIGR01208
TIGR01208   RmlA_long
SignalP No significant hit
TMHMM No significant hit
Mith_00050 Mith_00050   Mith_00070 Mith_00070
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