Madu_00220 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	37,087 / 35,858 / - [in whole cluster] 37,087 / 35,858 / - [in contig]
Location	complement(35858..37087) [in whole cluster] complement(35858..37087) [in contig]
Type	CDS
Length	1,230 bp (409 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	P450 oxidoreductase
Gene
Gene (GenBank)	mdpU1
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B0BLN1 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 mdpU1: P-450 oxidoreductase operonを形成すると推測されるP-450 oxidoreductases(MdpU1,MdpU3)とunknown protein MdpU2は、Maduropeptin生合成に関連する特異的機能がアサインできなかった。しかしながら、これらのORFsはenediyne coreをさらに加工するaziridine formationに関与するかもしれない。
Related Reference	ACC B3XWK3 PmId [18640605] Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene. (J Biosci Bioeng. , 2008) comment Streptomyces sp. A-1544_psmA Cytochrome P450 monooxygenase　 Pladienolide Bの16-hydroxylated derivativeであるpladienolide D産生を促進するための株Streptomyces bungoensis A-1544から分離したpladienolide B 16-hydroxylase PsmA。 PsmAの過剰発現で15倍のpladienolide Dを得た。 ferredoxin_psmBが一緒でないと変換率はぐっと低くなる。

Sequence

>putative cytochrome P450 [P450 oxidoreductase]
MTVSDVSASGLPEYPLARECPHRPGLGTASLREPGPMTKVRLYDGRTAWLVTGAEEARSL
LADQRTSVRPHPNFPVLNEELTQMRATREMAVEEEGGFASALFGVDPPEHSRQRQVLVPQ
FSAKRVARQRPNIQRIVDEQLDAMVREGAPADFITAFAEPVPTKVVCAHLGVPFSEHAAF
GEFASKLFDPVDADAAMDGLVAYLDDLVRRKESEPGTGMLDALIAEHVRPGRVERGELVQ
FALAVLVAGTVTTTSTIALGTLALLDAPGAYAALHADPSLAPGAVEEILRWVSLVEQISR
VALEDIELSGRTIEAGDGLLISTAGANWGPDVTSHPEEFDITRPPGRHLSFGHGIHHCLG
RNLARMELEVVFETLTRRLPGLRSVMPTAEIPAQHDGTVQKLLCFPVTW

>putative cytochrome P450 [P450 oxidoreductase]
ATGACCGTTTCGGACGTGAGCGCCTCCGGGCTTCCGGAGTACCCGCTCGCCAGGGAGTGC
CCGCACCGTCCGGGCCTCGGCACCGCGAGCCTCCGCGAGCCGGGGCCGATGACGAAGGTG
CGGCTGTACGACGGCCGCACCGCGTGGCTGGTCACCGGCGCGGAGGAGGCGCGGTCCCTG
CTGGCCGACCAGCGGACCTCCGTGCGCCCGCACCCGAACTTCCCGGTGCTGAACGAGGAA
CTGACGCAGATGCGGGCCACCCGGGAGATGGCCGTCGAGGAGGAGGGCGGGTTCGCCTCC
GCGCTGTTCGGCGTCGACCCGCCCGAGCACAGCCGCCAGCGGCAGGTGCTCGTCCCGCAG
TTCAGCGCCAAGCGCGTGGCCCGGCAGCGTCCGAACATCCAGCGCATCGTCGACGAGCAG
CTCGACGCGATGGTGCGGGAGGGCGCCCCGGCCGACTTCATCACCGCGTTCGCCGAGCCG
GTGCCGACGAAGGTGGTCTGCGCCCACCTCGGCGTCCCGTTCTCCGAGCACGCCGCCTTC
GGCGAGTTCGCGAGCAAGCTGTTCGACCCGGTGGACGCCGACGCGGCGATGGACGGACTG
GTCGCCTACCTCGACGACCTGGTGCGGCGCAAGGAGAGCGAGCCCGGCACCGGCATGCTC
GACGCGCTGATCGCCGAGCACGTCCGTCCGGGCCGGGTCGAACGCGGCGAGCTGGTGCAG
TTCGCCCTGGCGGTGCTGGTCGCCGGGACCGTCACCACCACCAGCACCATCGCGCTGGGC
ACGCTGGCGCTGCTGGACGCCCCCGGCGCGTACGCGGCCCTGCACGCCGACCCGAGCCTG
GCGCCCGGGGCGGTCGAGGAGATCCTGCGGTGGGTGTCGCTCGTCGAGCAGATCTCGCGG
GTGGCGCTGGAGGACATCGAGCTGTCCGGGCGGACCATCGAGGCGGGGGACGGGCTCCTC
ATCAGCACCGCCGGCGCCAACTGGGGCCCGGACGTGACGAGCCACCCCGAGGAGTTCGAC
ATCACGCGGCCCCCCGGGCGGCACCTCTCCTTCGGCCACGGGATCCACCACTGCCTCGGC
CGCAACCTCGCGCGGATGGAGCTGGAGGTGGTCTTCGAGACGCTGACCCGGCGCCTTCCG
GGGCTGCGGTCGGTGATGCCGACCGCGGAGATCCCCGCGCAGCACGACGGGACCGTGCAG
AAGCTCCTCTGCTTCCCCGTCACCTGGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 PR00385 P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Madu_00210 Madu_00230