Madu_00370 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	58,511 / 56,853 / - [in whole cluster] 58,511 / 56,853 / - [in contig]
Location	complement(56853..58511) [in whole cluster] complement(56853..58511) [in contig]
Type	CDS
Length	1,659 bp (552 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	oxidoreductase
Gene
Gene (GenBank)	mdpE9
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B0BLP6 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 mdpE9: Oxidoreductase 12ORFs(MdpE through MdpE11)がenediyne coreの生合成に関与するとしている。機能解析はされていない。
Related Reference	ACC P12676 PmId [2914858] Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp. (J Bacteriol. , 1989) [10194345] Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants. (Biochemistry. , 1999) comment Streptomyces sp._choA Cholesterol oxidase EC 1.1.3.6/5.3.3.1 [23%id, 2e-15] [PMID:2914858] choA geneのシーケンス解析。 secreted cholesterol oxidaseであると推定された。 [PMID:10194345] choAの結晶構造解析活性中心が同定されている。
Family/Domain	FD IPR000172, IPR007867 comment [IPR000172] Glucose-methanol-choline oxidoreductase, N-terminal (Domain) [IPR007867] Glucose-methanol-choline oxidoreductase, C-terminal (Domain)

Sequence

>putative oxidoreductase [oxidoreductase]
MTATESTDVLIIGSGFGGAIAAYHLAAGGADVVVLERGPWLEGDDFEHDFKLGSSYTRIF
DFVVGDGMSVLGGNCVGGGSVVYFAAMPRAPRFIFERRGGIGRRMWPESVSRDALEPWYD
RVEEALPVVKQSWDEVTYAGGLWAAACDHAGRTANPVPVAVDNSKCVNCNWMMAGCKFDA
KRSLLFNYLPAAVAEGAEIRPLHEVQRLTRNAGGGYRVHYNVIDGEDYRVHTGNGTIDAK
IVVLAAGSAATPVILQRSEDDLGRMPHAVGRYFSGNGERLNTAVVNEDKVREVLGLSRPD
GRAYAANQIGRGPVVASWDRLDGSLPEYSRFSLEQLYFPPGLGTILAQVPDAAGPTWFGA
EKKEMLRNWQSWLTIFTISEDDNEGVFGPPPATGNAMRISQQMLGRGNLHYRPTLNTMRG
WAESDAAVRDILERDGLAEVRPWTNDLVGAYTVHPLASCRIGDDPATSALDDRHELRGHP
GIFVTDGSAVPCGLTVNPAMTIAALAERAMPAIVRAARDRGVEVGYGAPAPDGSTAGRRA
TLPLAPAVGGDR

>putative oxidoreductase [oxidoreductase]
ATGACCGCAACGGAGAGCACCGACGTCCTGATCATCGGCAGCGGGTTCGGCGGCGCGATC
GCCGCCTACCACCTGGCCGCCGGCGGTGCCGACGTCGTCGTGCTGGAACGCGGCCCGTGG
CTGGAGGGCGACGACTTCGAGCACGACTTCAAGCTCGGCTCGTCCTACACCCGGATCTTC
GACTTCGTCGTCGGCGACGGCATGAGCGTGCTCGGCGGCAACTGCGTCGGGGGCGGCAGC
GTCGTGTACTTCGCGGCGATGCCGCGCGCGCCGCGGTTCATCTTCGAGCGGCGCGGCGGC
ATCGGCCGCCGGATGTGGCCGGAGAGCGTCTCCAGGGACGCGCTGGAACCGTGGTACGAC
CGGGTGGAGGAGGCGCTGCCGGTCGTCAAGCAGTCCTGGGACGAGGTCACCTACGCGGGC
GGGCTCTGGGCGGCGGCGTGCGACCACGCGGGCCGCACCGCGAACCCCGTGCCGGTCGCC
GTGGACAACTCCAAGTGCGTCAACTGCAACTGGATGATGGCCGGGTGCAAGTTCGACGCC
AAGCGGTCGCTGCTGTTCAACTACCTGCCCGCGGCCGTGGCGGAGGGCGCCGAGATCCGG
CCGCTGCACGAGGTCCAGCGGCTCACCCGCAACGCCGGCGGCGGCTACCGCGTCCACTAC
AACGTCATCGACGGCGAGGACTACCGGGTGCACACCGGGAACGGGACCATCGACGCCAAG
ATCGTGGTCCTGGCCGCCGGCAGCGCGGCGACGCCGGTGATCCTCCAGCGCTCCGAGGAC
GACCTCGGGCGGATGCCGCACGCGGTGGGCCGCTACTTCTCCGGCAACGGCGAGCGGCTG
AACACCGCGGTCGTCAACGAGGACAAGGTGCGGGAGGTGCTCGGCCTGTCGCGCCCCGAC
GGCCGCGCCTACGCCGCCAACCAGATCGGCCGCGGGCCGGTCGTGGCGAGCTGGGACCGG
CTCGACGGAAGCCTGCCCGAGTACTCGCGCTTCTCGCTGGAGCAGCTCTACTTCCCGCCC
GGCCTGGGCACCATCCTCGCCCAGGTCCCGGACGCCGCCGGGCCCACCTGGTTCGGCGCG
GAGAAGAAGGAGATGCTGCGGAACTGGCAGTCGTGGCTGACCATCTTCACCATCTCCGAG
GACGACAACGAGGGGGTCTTCGGGCCGCCGCCGGCGACCGGCAACGCCATGCGCATCTCG
CAGCAGATGCTCGGCCGCGGCAACCTGCACTACCGGCCGACGCTGAACACCATGCGCGGG
TGGGCCGAGTCCGACGCCGCCGTCCGGGACATCCTCGAACGCGACGGGCTGGCCGAGGTG
CGGCCGTGGACGAACGACCTGGTCGGCGCGTACACCGTGCATCCGCTCGCGTCCTGCCGG
ATCGGCGACGACCCCGCGACCTCGGCGCTGGACGACCGGCACGAGCTGCGGGGGCACCCC
GGGATCTTCGTCACCGACGGCTCGGCGGTGCCCTGCGGCCTGACCGTCAACCCGGCGATG
ACCATCGCCGCGCTGGCGGAGCGGGCCATGCCCGCGATCGTGCGCGCGGCGCGGGACCGG
GGCGTCGAGGTCGGGTACGGGGCGCCCGCGCCCGACGGCTCGACGGCCGGCCGCCGGGCC
ACGCTGCCGCTGGCGCCCGCGGTGGGCGGGGACCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000172 Glucose-methanol-choline oxidoreductase, N-terminal (Domain) PF00732 GMC_oxred_N IPR007867 Glucose-methanol-choline oxidoreductase, C-terminal (Domain) PF05199 GMC_oxred_C
SignalP	No significant hit
TMHMM	No significant hit

Madu_00360 Madu_00380