C1027_00170 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	21,402 / 19,921 / - [in whole cluster] 21,402 / 19,921 / - [in contig]
Location	complement(19921..21402) [in whole cluster] complement(19921..21402) [in contig]
Type	CDS
Length	1,482 bp (493 aa)

Annotation

Category	3.4 other modification
Product	2-amino-2-deoxyisochorismate synthase
Product (GenBank)	anthranilate synthase I
Gene	sgcD
Gene (GenBank)
EC number
Keyword	benzoxazolinate
Note
Note (GenBank)	ORF15
Reference	ACC Q8GMH4 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [18182490] Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. (Proc Natl Acad Sci U S A. , 2008) comment [PMID:12183628] C-1027の生合成遺伝子クラスターの報告 sgcD to sgcD6が、benzoxazolinate biosynthesisに関与するとしている。機能解析はしていない。 anthranilate I and II subunits (SgcD and SgcD1)としている。 [PMID:18182490] benzoxazolinate生合成に関与するSgcD,SgcGの機能解析 SgcDをcloning,大腸菌にて発現・精製し活性を調べている。 SgcDは、anthranilate synthase component I (ASI)が持つpyruvate lyase activityを欠いていた。 HPLC解析は、SgcDがコリスミン酸を2-amino-2-deoxyisochorismate(ADIC)のスペクトルを示す産物へと変換させたことを示した。

Sequence

>2-amino-2-deoxyisochorismate synthase [anthranilate synthase I]
MTDQCVVSAPVRVRTRRLDVKETGALPAYRALAEHFGPDEVYLLESAAGPARDRRHQFVG
FGALLSLSVTDRVVRVEGVPALRGLLLERAGALLEDGPQGLRLRTAGGLWPLLRAMRDMF
DAEGSASGFRFGFLGFFGYDTARYIEDLPHLIENRPGLPDVRMVLHRGSVVTDLATGRCE
LLLHESPYWPGLAPETVTGLLADVEQAWPDPSADGFPASAVTDDSAPEVFANDVERCLKH
IAVGDIYQVQIGHELSIRSTADPADVYQRLRGRNASPYMYLAGIDGHRLIGASPELFVRI
EDGEVTMRPIAGTVPRSGADGGIAAGVRLRSDPKEIAEHTMLVDLCRNDIGRIARPNTLD
VPDQLDVEGYSHVLHLVSTVVGRARVDTDAFDTIAALFPAGTMTGAPKIRAMEIIESVER
SRRGLYAGALGLLDVGGYTNLALCIRTLFHHEGVYRTRASAGIVADSEPGAEWTETLAKM
SATHWAVTGEELL

>2-amino-2-deoxyisochorismate synthase [anthranilate synthase I]
ATGACCGACCAGTGCGTGGTCTCGGCCCCGGTGCGGGTGCGGACACGACGCCTCGATGTG
AAGGAGACGGGCGCCCTGCCCGCCTACCGCGCGCTGGCCGAGCACTTCGGCCCCGACGAG
GTCTACCTCCTGGAGTCCGCGGCCGGCCCGGCCCGCGACCGCAGGCACCAGTTCGTCGGC
TTCGGCGCGCTGCTCTCGCTCTCGGTCACCGACCGGGTGGTGCGCGTCGAGGGCGTACCC
GCCCTGCGGGGCCTGCTCCTGGAGCGGGCCGGCGCGCTCCTGGAGGACGGCCCCCAGGGG
CTGCGGCTGCGTACCGCGGGCGGGTTGTGGCCGCTGCTGCGGGCCATGCGGGACATGTTC
GACGCCGAGGGGTCCGCGTCCGGCTTCCGCTTCGGGTTCCTCGGGTTCTTCGGGTACGAC
ACGGCCCGCTACATCGAGGACCTGCCCCACCTCATCGAGAACCGGCCTGGCCTGCCGGAC
GTACGCATGGTCCTGCACCGGGGCAGCGTGGTCACCGATCTCGCCACCGGCCGATGCGAA
CTGCTGCTCCACGAGTCCCCGTACTGGCCCGGCCTCGCGCCGGAGACCGTCACCGGGCTC
CTCGCCGACGTGGAGCAGGCCTGGCCCGATCCGTCCGCAGACGGCTTCCCCGCCTCCGCG
GTCACCGACGACAGCGCCCCCGAAGTGTTCGCGAACGACGTCGAGCGGTGTCTGAAGCAC
ATCGCCGTCGGCGACATCTACCAGGTGCAGATCGGTCACGAGCTCTCCATCCGCTCGACG
GCCGACCCCGCGGACGTCTACCAGCGCCTGCGCGGGCGGAACGCCTCCCCGTACATGTAT
CTGGCGGGCATCGACGGGCACCGTCTGATCGGCGCCAGCCCCGAGCTGTTCGTACGCATC
GAGGACGGCGAGGTCACGATGCGGCCGATCGCGGGCACGGTGCCGCGGTCCGGCGCGGAC
GGCGGCATCGCCGCGGGAGTCCGGCTGAGGTCGGATCCCAAGGAGATCGCCGAGCACACC
ATGCTGGTGGACCTCTGCCGCAACGACATCGGACGGATCGCCCGGCCGAACACGCTCGAC
GTACCCGACCAGCTGGACGTGGAGGGCTACTCCCACGTGCTGCACCTGGTCTCCACGGTC
GTCGGCCGGGCGCGGGTGGACACCGATGCCTTCGACACCATCGCCGCCCTGTTCCCCGCC
GGCACCATGACGGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGAGTCCGTCGAACGC
AGCCGCCGCGGCCTGTACGCCGGGGCGCTCGGCCTCCTCGACGTGGGCGGGTACACCAAC
CTCGCGCTGTGCATCCGGACCCTTTTCCACCACGAGGGCGTCTACCGCACCCGGGCCTCG
GCCGGAATCGTCGCCGATTCCGAACCGGGGGCGGAGTGGACCGAGACGCTGGCCAAGATG
AGCGCCACTCACTGGGCGGTCACCGGAGAGGAGTTGCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005801 ADC synthase (Domain) G3DSA:3.60.120.10 TRPE_1_chor_bd SSF56322 TRPE_1_chor_bd IPR006805 Anthranilate synthase component I, N-terminal (Domain) PF04715 Anth_synt_I_N IPR015890 Chorismate binding, C-terminal (Domain) PF00425 Chorismate_bind IPR019999 Anthranilate synthase component I (Family) PR00095 ANTSNTHASEI
SignalP	No significant hit
TMHMM	No significant hit

C1027_00160 C1027_00180