C1027_00180 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	22,873 / 21,419 / - [in whole cluster] 22,873 / 21,419 / - [in contig]
Location	complement(21419..22873) [in whole cluster] complement(21419..22873) [in contig]
Type	CDS
Length	1,455 bp (484 aa)

Annotation

Category	3.4 other modification
Product	putative ligase
Product (GenBank)	putative phenylacetyl CoA ligase
Gene	sgcD5
Gene (GenBank)
EC number
Keyword	benzoxazolinate
Note
Note (GenBank)	ORf14
Reference	ACC Q8GMH3 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [18182490] Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. (Proc Natl Acad Sci U S A. , 2008) comment [PMID:12183628] C-1027の生合成遺伝子クラスターの報告 sgcD to sgcD6, encoding benzoxazolinate biosynthesis. 機能解析はしていない。 CoA ligase (SgcD5)としている。 [PMID:18182490] この論文内でbenzoxazolinate生合成推定経路が示されており、benzoxazolinate moiety生合成の3段階目に関与すると推定している。この論文のdiscussion内で、amide bond formationに関与するORFだと記述している。SgcD5の機能解析はしていない。
Related Reference	ACC O33469 PmId [8969218] Molecular cloning and expression in different microbes of the DNA encoding Pseudomonas putida U phenylacetyl-CoA ligase. Use of this gene to improve the rate of benzylpenicillin biosynthesis in Penicillium chrysogenum. (J Biol Chem. , 1996) comment Pseudomonas putida_paaK(paaF, pcl) Phenylacetate-coenzyme A ligase (phenylacetyl-CoA ligase) 27%id, 3e-15 phenylacetyl-CoA ligaseをコードするpclをcloningし大腸菌にて発現、 phenylacetyl-CoA ligase activityをみている。
Family/Domain	FD IPR000873 comment [IPR000873] AMP-dependent synthetase/ligase (Domain)

Sequence

>putative ligase [putative phenylacetyl CoA ligase]
MRDGRRPGPGGTGPLDPDADSEFWSAYGQVSDAFYRGELTAADRERWEGERLTAVLRHVT
RRSPFYRRHLAGVDVEAVTPANLADLPFTTKDDLRREMHDVLSGPLHEARIYYETTGTTG
AATPCPRGEKDIATSNIAVRESWRRMLEARFGGRMPVVGLMGPSELYAFGDVFTAVAAEL
GACHVKIWPESPRVGFRKALRLIEELEVEVVVCAPALCLSLAKAALHYGYDLARLPVKLF
LTLGEICTPQFADNVATLWPQAVVRPTLYGSQEALCIATGADTGALHLAQPNYLTELVEP
DTGAVVGDTGEGELVLTMLVDGIKPLIRYRTGDLVRILPAGPGEPLPGPRIQVIGRVADR
IPLGDVTLQPAELEAAILDGVGGCLGYQVVIDRQDDGSDAVTVRMDLLAGAEGERQGIGE
AVAARLRERTGAHAGIVVDTDLDPVTHTGSFVSWKAARVVDNRSGPDRAVLTARQVAHRY
AITT

>putative ligase [putative phenylacetyl CoA ligase]
GTGAGAGACGGCCGGCGGCCCGGTCCCGGCGGGACCGGGCCGCTGGACCCCGACGCGGAC
AGCGAGTTCTGGTCCGCCTACGGGCAGGTGAGCGACGCGTTCTACCGCGGCGAACTCACC
GCCGCCGACCGCGAGCGCTGGGAGGGCGAACGGCTGACGGCCGTACTGCGCCACGTCACC
CGCCGCTCGCCGTTCTACCGCCGCCATCTGGCAGGCGTGGACGTCGAGGCCGTCACCCCC
GCGAACCTGGCGGACCTGCCCTTCACCACCAAGGACGACCTGCGGCGGGAGATGCACGAC
GTCCTGTCGGGCCCGCTGCACGAAGCACGCATCTACTACGAGACCACCGGGACCACCGGG
GCGGCCACCCCGTGCCCACGCGGCGAGAAGGACATCGCGACCAGCAACATCGCCGTCCGG
GAGTCCTGGCGCCGCATGCTGGAAGCGAGGTTCGGGGGGCGGATGCCCGTCGTGGGCCTG
ATGGGGCCGTCGGAGCTGTACGCGTTCGGGGACGTGTTCACCGCGGTGGCAGCGGAGCTG
GGGGCCTGTCACGTCAAGATCTGGCCCGAGTCCCCCAGGGTGGGATTCCGTAAGGCGCTG
CGGCTGATCGAGGAGCTGGAGGTCGAGGTCGTCGTCTGTGCTCCGGCGCTGTGCCTGAGC
CTTGCCAAGGCGGCCCTGCACTACGGCTACGACCTGGCCCGGCTTCCCGTGAAGCTCTTC
CTCACGCTGGGTGAGATCTGCACCCCGCAGTTCGCCGACAACGTGGCGACCCTCTGGCCG
CAAGCCGTCGTGCGGCCCACGCTCTACGGCTCCCAGGAAGCCCTGTGCATCGCCACCGGA
GCGGACACCGGAGCGCTCCACCTCGCCCAGCCCAACTACTTGACCGAACTCGTCGAACCG
GACACCGGCGCCGTGGTCGGCGACACGGGAGAGGGCGAACTCGTCCTCACCATGCTGGTG
GACGGCATCAAACCACTGATCCGCTACCGCACCGGGGACCTGGTCCGCATCCTCCCGGCG
GGCCCCGGGGAGCCGCTGCCGGGACCGCGGATCCAGGTCATCGGCCGGGTCGCGGACCGC
ATCCCCCTGGGCGACGTGACCCTCCAGCCCGCCGAGCTGGAGGCCGCGATCCTCGACGGG
GTCGGCGGCTGCCTCGGCTACCAGGTCGTCATCGACCGGCAGGACGACGGCAGCGACGCC
GTCACCGTGCGCATGGACCTGCTGGCCGGCGCCGAGGGGGAACGGCAGGGCATCGGCGAG
GCCGTCGCCGCCCGGCTCAGGGAGCGGACCGGCGCGCACGCCGGCATCGTCGTGGACACC
GACCTCGACCCCGTGACCCACACCGGCTCCTTCGTCAGCTGGAAGGCCGCGCGCGTCGTC
GACAACCGCTCCGGCCCCGACCGGGCGGTGCTGACCGCCCGTCAGGTGGCACACCGCTAC
GCCATCACCACGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding
SignalP	No significant hit
TMHMM	No significant hit

C1027_00170 C1027_00190