C1027_00290 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	35,933 / 35,511 / - [in whole cluster] 35,933 / 35,511 / - [in contig]
Location	complement(35511..35933) [in whole cluster] complement(35511..35933) [in contig]
Type	CDS
Length	423 bp (140 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)	unknown
Gene	sgcJ
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF18
Reference	ACC Q8GMG4 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [27406907] Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027. (J Antibiot (Tokyo). , 2016) comment [PMID: 12183628](2002) C-1027の生合成遺伝子クラスターの報告 Fig.2にてcluster内に表示されているのみ特にふれていない。 --- [PMID: 27406907](2016) SgcJの結晶構造解析、遺伝子不活化、site-directed mutagenesis調査。 SgcJはC-1027産生に必要。

Sequence

>hypothetical protein [unknown]
MTSTDSTTSAPDAALAAVAALPARIVAAWADHDADRFADVFAEDGTMILPGLFRKGRENI
RTHMAAAFAGPYKGTRVIGSPIDARLLGDGIALLITEGGILAPGETEASGDGAVRASWLA
VEQDGQWRLAAYQNSPRGND

>hypothetical protein [unknown]
ATGACCAGCACCGACTCGACCACCTCCGCTCCCGACGCCGCCCTGGCAGCGGTAGCCGCT
CTGCCGGCGCGCATCGTGGCCGCCTGGGCCGACCACGACGCCGACCGGTTCGCCGACGTC
TTCGCCGAGGACGGGACGATGATCCTCCCCGGCCTCTTCCGCAAGGGCCGCGAGAACATC
CGCACCCACATGGCGGCCGCCTTCGCGGGCCCGTACAAGGGCACCCGTGTCATCGGCAGC
CCCATCGACGCGCGCCTGCTCGGCGACGGCATCGCCCTGCTCATCACCGAGGGCGGCATC
CTCGCCCCGGGGGAGACCGAGGCGAGCGGCGACGGCGCCGTCCGGGCCTCCTGGCTGGCC
GTCGAACAGGACGGGCAGTGGCGTCTGGCCGCTTACCAGAACAGCCCGCGCGGCAACGAC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011944 Steroid delta5-4-isomerase (Family) TIGR02246 TIGR02246
SignalP	No significant hit
TMHMM	No significant hit

C1027_00280 C1027_00300