C1027_00310 C1027_00310   C1027_00330 C1027_00330

C1027_00320 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction38,978 / 39,259 / + [in whole cluster]
38,978 / 39,259 / + [in contig]
Location38978..39259 [in whole cluster]
38978..39259 [in contig]
TypeCDS
Length282 bp (93 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase peptidyl carrier protein
Product (GenBank)type II PCP
GenesgcC2
Gene (GenBank)
EC number
Keyword
  • (S)-3-chloro-4,5-dihydroxy-beta-phenylalanine
Note
Note (GenBank)
  • ORF26
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
[16104723] Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates. (J Am Chem Soc. , 2005)
comment
[PMID:12183628]
C-1027の生合成遺伝子クラスターの報告

sgcC to sgcC5, encoding beta-amino biosynthesis.
機能解析はしていない。
NRPS peptidyl-carrier protein(SgcC2)としている。


[PMID:16104723]
C-1027のbeta-amino acid moiety生合成の報告

apo-sgcC2をCoA存在下でphosphopanteinyl transferaseと反応させた時のFT-MS値は、active site Serineがphosphopantheinylationされた時の計算値と一致した。また、holo-sgcC2にATP存在下で7(SgcC1反応産物)、3(tyrosineとSgcCの反応産物)とSgcC4のmixtureをそれぞれ反応させると、(S)-beta-tyrosinyl-S-SgcC2中間体のmassと一致したことを見ている。

close this sectionPKS/NRPS Module

B1
PCP13..79

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase peptidyl carrier protein [type II PCP]
MSTVSDTAAGSSLEEKVTRIWTGVLGTSGEEGATFIELGGQSVSAVRIATRIQEELDIWV
DIGVLFDDPDLPTFIAAVVRTADAAGGEGSGTQ
selected fasta
>non-ribosomal peptide synthetase peptidyl carrier protein [type II PCP]
GTGTCCACCGTTTCCGACACAGCGGCCGGCTCCTCCCTGGAGGAGAAGGTCACCCGGATC
TGGACGGGTGTTCTCGGCACGTCCGGTGAGGAAGGCGCGACGTTCATCGAGCTCGGAGGG
CAGTCGGTCTCGGCCGTGCGCATCGCCACGCGTATCCAGGAGGAGCTCGACATCTGGGTC
GACATCGGCGTCCTCTTCGACGACCCGGATCTGCCTACCTTCATCGCGGCGGTCGTCCGG
ACGGCCGACGCCGCGGGCGGCGAGGGCTCCGGAACGCAGTGA
[1] PCP13..79
[1] PCP37..237

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[5-79] 1.6e-12 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[11-83] 6.899999633797e-10 SSF47336
SSF47336   ACP_like
[13-79] PS50075
PS50075   ACP_DOMAIN
[15-77] 7.80000000000001e-08 PF00550
PF00550   PP-binding
SignalP No significant hit
TMHMM No significant hit
C1027_00310 C1027_00310   C1027_00330 C1027_00330
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