C1027_00490 C1027_00490   C1027_00510 C1027_00510

C1027_00500 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction64,131 / 62,782 / - [in whole cluster]
64,131 / 62,782 / - [in contig]
Locationcomplement(62782..64131) [in whole cluster]
complement(62782..64131) [in contig]
TypeCDS
Length1,350 bp (449 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)P450 hydroxylase
GenesgcE7
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF42
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
comment
C-1027の生合成遺伝子クラスターの報告

SgcE6, SgcE7, SgcE9 は様々なoxidoreductaseに相同性を示すと記述あり。
SgcE7の機能解析はされていない。
Related Reference
ACC
O23365
PmId
[19939422] Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a beta-carotene ketolase provides insight into in vivo functions. (Phytochemistry. , 2010)
comment
Arabidopsis thaliana_CYP97B3
Cytochrome P450 97B3
25%id 7e-41

ferredoxin-dependent non-heme di-iron monooxygenases(AtB1)とheme-containing cytochrome P450(CYP) monooxygenases(CYP97A3 and CYP97C1) とhydroxylase候補(CYP97B3)の過剰発現株を作製。alpha-caroteneとbeta-caroteneのbeta-, epsilon-rings の修飾におけるhydroxylase classesの役割を調べた。
foliar carotenoid profiles分析は、CYP97B3がcarotenoid biosynthesisに関与することを示した。そしてknock-out mutantsの解析によりhydroxylase activitiesを確かめた。carotenoid biosynthesisにおいて、multifunctional enzymesとして機能すると示唆された。
Family/Domain
FD
IPR001128
comment
[IPR001128] Cytochrome P450 (Family)

close this sectionSequence

selected fasta
>putative cytochrome P450 [P450 hydroxylase]
MRIDPPGPPLRALPGLLRKLAVDRLGMMRDAAGLGDAVRVSMGPKKLYIFNRPDYAKHVL
ADNSDNYHKGIGLVQSRRVLGDGLLTSDGETWREQRRIVQPAFKPGRINQQAAAVAEEAA
KLVALLRGHEGGGPVDVLQEVTGLTLGVLGRTLLDSNLTAHESLAHSFEEVQDQAMLEMV
SQGTVPAWLPLPPQARFRRARRELYRVADLLVADRRSRMADGGPGDDALSRIIVAADRRR
DDPARARNRLREELVTLLLAGHETTASTLGWTLHLLERHPEVRDRVRAEARAALGDGVPG
PEDLHRLTYTTMVVQEAMRLFPPVWILPRVAQQRDVVGGYTVSAGSDVLVCPYIMHRHPG
LWEDPERFDPERFEPRQTADRPRYAYIPFGAGPRFCVGSNLGMMEAVFVTALVTRDLDLR
TVAGHRAVAEPMLSLRMRGGLPMTVSTAR
selected fasta
>putative cytochrome P450 [P450 hydroxylase]
ATGAGGATCGACCCGCCCGGCCCGCCCCTCCGGGCGCTTCCGGGACTGCTGAGAAAGCTG
GCGGTGGACCGCCTCGGGATGATGAGGGACGCGGCCGGGCTCGGGGACGCCGTCCGCGTC
TCCATGGGGCCCAAGAAGCTGTACATCTTCAACCGCCCCGACTACGCCAAGCACGTCCTG
GCGGACAACAGCGACAACTACCACAAGGGCATCGGTCTGGTGCAGTCCCGCCGGGTGCTG
GGCGACGGGCTGCTCACCAGTGACGGTGAGACGTGGCGCGAACAGCGCAGGATCGTGCAG
CCGGCGTTCAAACCGGGCCGCATCAACCAGCAGGCGGCCGCCGTCGCCGAGGAGGCGGCC
AAGCTGGTCGCCCTGCTGCGCGGTCACGAGGGGGGCGGTCCCGTGGACGTTCTCCAGGAG
GTCACCGGTCTCACCCTAGGCGTACTGGGCCGGACCCTGCTCGACTCGAACCTCACCGCG
CACGAGTCGCTCGCCCACTCCTTCGAGGAGGTCCAGGACCAGGCCATGCTGGAAATGGTG
AGCCAGGGCACGGTGCCGGCCTGGCTGCCGCTGCCTCCGCAAGCACGCTTCCGGCGCGCC
CGCAGGGAGCTGTACCGGGTGGCCGACCTGCTGGTGGCCGACCGGCGCTCCAGGATGGCC
GACGGCGGGCCGGGCGACGACGCGCTGTCCCGGATCATCGTCGCCGCGGACAGGCGCCGG
GACGACCCCGCCCGGGCTCGCAACAGGCTGCGCGAGGAACTGGTCACCCTGCTCCTGGCG
GGCCACGAGACCACTGCCAGTACGCTCGGCTGGACCCTGCATCTGCTGGAACGTCACCCC
GAGGTACGTGACCGGGTCCGCGCGGAGGCCCGTGCCGCCCTCGGCGACGGCGTCCCCGGA
CCGGAGGACCTGCATCGGCTGACGTACACCACGATGGTGGTGCAGGAGGCGATGCGGCTG
TTCCCTCCCGTGTGGATCCTGCCCAGGGTGGCCCAGCAACGCGATGTGGTCGGCGGCTAC
ACGGTGTCCGCCGGATCGGATGTGCTGGTCTGCCCGTACATCATGCACCGCCATCCGGGG
CTGTGGGAGGACCCCGAGCGCTTCGACCCCGAGCGGTTCGAACCCCGGCAGACCGCCGAC
CGGCCGCGGTACGCGTACATCCCGTTCGGCGCGGGGCCCCGGTTCTGCGTCGGCAGCAAC
CTCGGGATGATGGAGGCGGTGTTCGTCACGGCCCTGGTCACCCGCGATCTCGATCTGCGT
ACCGTCGCCGGACACCGGGCGGTTGCCGAACCCATGCTCTCCCTGCGGATGCGCGGAGGG
CTACCGATGACGGTGAGTACGGCGAGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[260-277] 2.09999806691534e-13 PR00385 [312-323] 2.09999806691534e-13 PR00385 [387-396] 2.09999806691534e-13 PR00385 [396-407] 2.09999806691534e-13 PR00385
PR00385   P450
[32-420] 4.2e-76 PF00067
PF00067   p450
[5-446] 2.99999999999998e-110 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[5-445] 3.49999466863949e-112 SSF48264
SSF48264   Cytochrome_P450
IPR002401 Cytochrome P450, E-class, group I (Family)
[31-50] 2.19999900980708e-21 PR00463 [249-266] 2.19999900980708e-21 PR00463 [269-295] 2.19999900980708e-21 PR00463 [351-375] 2.19999900980708e-21 PR00463 [386-396] 2.19999900980708e-21 PR00463 [396-419] 2.19999900980708e-21 PR00463
PR00463   EP450I
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[389-398] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
C1027_00490 C1027_00490   C1027_00510 C1027_00510
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