C1027_00540 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	73,628 / 71,700 / - [in whole cluster] 73,628 / 71,700 / - [in contig]
Location	complement(71700..73628) [in whole cluster] complement(71700..73628) [in contig]
Type	CDS
Length	1,929 bp (642 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)	unknown
Gene	sgcE4 unbV
Gene (GenBank)
EC number
Keyword	enediyne core
Note
Note (GenBank)	ORF48
Reference	ACC Q8GMD9 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003) comment [PMID:12183628] C-1027の生合成遺伝子クラスターの報告 Fig.2にてcluster内に表示されているのみ。特に記述なし。 [PMID:12536216] warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)の報告 warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。C-1027ではTEBC(thioesterase)のみが離れて存在する。 UNBVの構造解析は、N末端シグナル配列と共にsecreted proteinを持っていた。
Related Reference	ACC Q84HJ0 NITE Dynm_00160 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Micromonospora chersina_dynU14 DynU14 dynemicin生合成遺伝子 dynE8, orf8, dynU15, dynU14 and orf23の欠損株を作製し、培養液中の産物をHPLCにて検出。orf8はdynemicin Aが検出できたが、dynE8, dynU15, dynU14，orf23の欠損株はdynemicin Aが検出できなかった。ただし、orf23の欠損株はdynemicin Aの新規化合物が検出された。よって、dynE8, dynU15, dynU14はdynemicin Aに必須であることを示した。 dynU15, U14, T3, E8, E7は、minimal enediyne cassetteとして知られている遺伝子。 DynU15, U14, T3はconserved unknown proteins。

Sequence

>hypothetical protein [unknown]
MTHPISWLRKQAPGVVALAVMVGTFYAVKPSESSAAEKKEMAESFTFEPMAISMPGGYKK
QSVRKVNKAYKHIEAWISSVGAGVAMNDIDGDGLPNDLCITDPQIDQTVVTPAPAPGRES
PTYAPFVLDPAPLPKSDIMAPIGCVPGDFNEDGAMDLLVYYWGRTPVIFQAERKPGEDTT
AMDPKSFKPVELVPGSNGSTYKGPLWNSNAATVADFDGDGHNDIYIGNYFPDSPVLDDSV
HGGVTMNDSLSHAQNGGGGHFFRWTPSGFEEVKNVLPKGIDRGWTLAVSATDLDGDQRPE
MYLAHDFGTSALLYNRSTPGTFKFSEVKAVHTATTPKSKEIGRSSFKGMGVDFGDLDNDG
LYDAFVSNITTSFGIQESNFAFVATARDKADLRAKFRDGVAPYKDESAPLNLAWSGWGWD
VKMGDFDNNGIQEITQAVGFVKGKRNRWAQLQELATANDALVKHPRFWPRVEEGDDLAGD
QHLRFFVQDKDGEAYSDLSKQLGLAVPVPSRGIATGDADGDGRLDMVVARQWEDPVFYCN
MSKDTGEYLNLSLVDEAGSPVIGAQVTVTLSDGSTRLGRVDGGSGHSGKRSQDVHIGLGQ
EADGPMETRLTWRDRTGQVRNKDLQLTPGRHALELGADVKEK

>hypothetical protein [unknown]
ATGACACATCCAATTTCGTGGCTGCGGAAGCAGGCTCCGGGGGTCGTCGCACTCGCTGTC
ATGGTCGGCACTTTCTACGCGGTGAAGCCGTCGGAGTCGAGCGCCGCCGAGAAAAAGGAA
ATGGCGGAGAGTTTCACTTTCGAGCCGATGGCGATATCCATGCCTGGCGGTTACAAGAAA
CAGAGCGTCCGCAAGGTCAACAAGGCCTACAAGCACATCGAGGCGTGGATCTCCTCCGTC
GGCGCCGGCGTCGCCATGAACGACATCGACGGCGACGGCCTGCCCAACGACCTCTGCATC
ACCGATCCGCAGATCGACCAGACCGTCGTCACCCCGGCCCCGGCGCCCGGTCGCGAGAGT
CCCACGTACGCCCCCTTCGTGCTCGACCCGGCTCCGCTGCCCAAGAGCGACATCATGGCG
CCGATCGGCTGCGTCCCCGGGGACTTCAACGAGGACGGCGCGATGGACCTGCTCGTCTAC
TACTGGGGCCGCACCCCGGTGATCTTCCAGGCGGAGAGGAAGCCGGGCGAGGACACCACG
GCGATGGACCCGAAGTCCTTCAAGCCCGTCGAACTGGTTCCCGGCAGCAACGGCAGCACC
TACAAGGGCCCCCTGTGGAACTCCAACGCCGCGACGGTCGCCGACTTCGACGGCGACGGT
CACAATGACATCTACATCGGGAACTACTTCCCCGACAGCCCCGTCCTGGACGACAGCGTG
CACGGCGGCGTGACGATGAACGACTCGCTCTCCCACGCCCAGAACGGCGGCGGCGGTCAC
TTCTTCCGCTGGACCCCCTCCGGCTTCGAGGAGGTCAAGAACGTCCTGCCGAAGGGCATC
GACCGGGGCTGGACACTTGCCGTCTCCGCCACCGACCTGGACGGCGACCAGCGCCCCGAG
ATGTACCTGGCGCACGACTTCGGAACCTCGGCGCTGCTGTACAACAGGTCGACCCCCGGA
ACGTTCAAGTTCAGCGAGGTGAAGGCCGTACACACCGCGACCACTCCGAAGTCCAAGGAG
ATCGGCCGCAGCTCCTTCAAGGGCATGGGCGTCGACTTCGGTGATCTGGACAACGACGGC
CTGTACGACGCCTTCGTCTCCAACATCACCACCTCCTTCGGCATCCAGGAGTCGAACTTC
GCCTTCGTCGCGACCGCTCGCGACAAGGCGGATCTGCGGGCGAAGTTCCGTGACGGCGTC
GCCCCCTACAAGGACGAGAGCGCACCTCTCAACCTCGCCTGGTCCGGTTGGGGCTGGGAC
GTGAAGATGGGGGACTTCGACAACAACGGAATCCAGGAGATCACCCAGGCGGTCGGCTTC
GTCAAGGGCAAGCGCAACCGCTGGGCCCAGCTCCAGGAGCTCGCCACCGCCAACGACGCG
CTGGTCAAGCACCCCCGCTTCTGGCCCCGGGTGGAGGAGGGCGACGACCTCGCCGGCGAT
CAGCACCTGCGCTTCTTCGTACAGGACAAGGACGGCGAGGCCTACAGCGACCTGTCCAAG
CAGCTCGGTCTGGCTGTACCCGTGCCCAGCCGGGGCATCGCCACCGGCGACGCCGACGGG
GACGGCCGCCTGGACATGGTGGTCGCCCGCCAGTGGGAGGACCCGGTCTTCTACTGCAAC
ATGAGCAAGGACACCGGGGAATACCTCAACCTCAGCCTCGTCGACGAGGCCGGATCGCCC
GTGATCGGCGCGCAGGTCACCGTGACCCTGTCCGACGGCAGCACCCGCCTGGGCCGCGTC
GACGGCGGCAGCGGCCACTCCGGCAAGCGCAGCCAGGACGTGCACATCGGACTCGGCCAG
GAGGCCGACGGCCCGATGGAGACCCGTCTGACCTGGCGCGACCGCACCGGTCAGGTGCGC
AACAAGGACCTCCAGCTGACTCCCGGCAGGCACGCGCTGGAACTCGGCGCCGACGTGAAG
GAGAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011519 ASPIC/UnbV (Domain) PF07593 UnbV_ASPIC
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

C1027_00530 C1027_00550