C1027_00540 C1027_00540   C1027_00560 C1027_00560

C1027_00550 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction74,611 / 73,625 / - [in whole cluster]
74,611 / 73,625 / - [in contig]
Locationcomplement(73625..74611) [in whole cluster]
complement(73625..74611) [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)hypothetical protein
GenesgcE3
unbL
Gene (GenBank)
EC number
Keyword
  • enediyne core
Note
Note (GenBank)
  • ORF49
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
[12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003)
comment
[PMID:12183628]
C-1027の生合成遺伝子クラスターの報告

ORF49=sgcE3
sgcE3は高く保存されているものの、機能のわかっているものとの相同性は見られないと記述あり。


[PMID:12536216]
warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)の報告

warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。C-1027ではTEBC(thioesterase)のみが離れて存在する。
Related Reference
ACC
Q84HJ1
NITE
Dynm_00150
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Micromonospora chersina_dynU15
DynU15
Dynemicin生合成遺伝子

dynE8, orf8, dynU15, dynU14 and orf23の欠損株を作製し、培養液中の産物をHPLCにて検出。orf8はdynemicin Aが検出できたが、dynE8, dynU15, dynU14，orf23の欠損株はdynemicin Aが検出できなかった。ただし、orf23の欠損株はdynemicin Aの新規化合物が検出された。
よって、dynE8, dynU15, dynU14 はdynemicin Aに必須であることを示した。

dynU15, U14, T3, E8, E7は、minimal enediyne cassetteとして知られている遺伝子。
DynU15, U14, T3はconserved unknown proteins。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MSMLRALRRRILTPSVRETQLETRGFHIKDAEAKHQLETVGTSFLQGYAYAVEARSASQA
VDWLETVPRAFRGFAYEGAGMGAVMLGSLTGSSRRLTGILEGEGRRHNYMIYVGIGWAMA
RLPKFLWPDVTATDPVLRWLILDGYGFHQAYFKTDSYVRNPAAEHPFTWKGGPDAYSARA
IDQGIGRAMWFVGGTDPDVVADLIGTFPEHRHADLYAGAGLACTYAGSVDGDELRRFAKH
AGDHLPSLVQGSAFACEARERAGTTTAHTHLAAQILCGGRTPAEAAQVCTDARPATCAGG
ETPAFETWRQQIAATISSIPPTQKGAVA
selected fasta
>hypothetical protein [hypothetical protein]
GTGTCCATGTTGCGAGCGCTGAGGCGCCGAATTCTCACTCCCAGTGTTCGAGAAACACAG
CTGGAAACACGGGGTTTCCACATCAAGGACGCGGAGGCCAAGCACCAGTTGGAGACGGTC
GGGACGAGCTTCCTGCAGGGGTACGCGTACGCGGTCGAGGCCCGTTCGGCGAGCCAGGCC
GTCGACTGGCTGGAGACCGTGCCCCGGGCCTTTCGCGGGTTCGCCTACGAGGGCGCGGGC
ATGGGTGCGGTCATGCTCGGCTCCCTCACCGGGAGCAGTCGGCGGCTGACGGGGATCCTG
GAGGGCGAGGGCCGGCGTCACAACTACATGATCTACGTCGGCATCGGGTGGGCAATGGCC
CGGCTCCCCAAGTTCCTGTGGCCCGACGTGACCGCGACCGACCCGGTGCTGCGCTGGCTG
ATCCTGGACGGGTACGGCTTCCACCAGGCGTACTTCAAGACGGACTCCTACGTTCGCAAT
CCCGCCGCCGAGCACCCCTTCACCTGGAAGGGCGGCCCGGACGCCTACAGCGCGCGCGCC
ATCGACCAGGGCATCGGCCGGGCCATGTGGTTCGTCGGGGGCACCGACCCCGACGTCGTG
GCCGACCTCATCGGCACGTTCCCCGAGCACCGCCACGCCGACCTCTACGCGGGCGCCGGG
CTGGCCTGCACCTACGCGGGAAGCGTGGACGGGGACGAACTGCGGCGCTTCGCCAAGCAC
GCGGGCGACCATCTGCCCAGCCTCGTACAGGGCTCCGCTTTCGCCTGCGAGGCGCGGGAG
CGGGCGGGGACGACAACCGCCCACACGCACCTGGCCGCCCAGATCCTGTGCGGCGGCCGG
ACCCCGGCGGAAGCCGCACAGGTGTGCACGGACGCACGGCCCGCCACCTGTGCCGGCGGG
GAGACCCCGGCCTTCGAGACCTGGCGGCAGCAGATCGCCGCCACCATCAGTTCCATTCCT
CCCACGCAGAAGGGCGCCGTCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR012964 Protein of unknown function DUF1702 (Family)
[1-317] PF08012
PF08012   DUF1702
SignalP No significant hit
TMHMM No significant hit
C1027_00540 C1027_00540   C1027_00560 C1027_00560
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