Calm_00110 : CDS information

Location

Organism	Micromonospora echinospora
Strain	NRRL 15839
Entry name	Calicheamicin
Contig	AF497482
Start / Stop / Direction	10,158 / 11,081 / + [in whole cluster] 10,158 / 11,081 / + [in contig]
Location	10158..11081 [in whole cluster] 10158..11081 [in contig]
Type	CDS
Length	924 bp (307 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	NDP-hexose C-4 ketoreductase
Product (GenBank)	CalS2
Gene
Gene (GenBank)	calS2
EC number
Keyword	3-methoxy-L-rhamnose
Note
Note (GenBank)
Reference	ACC Q8KNI6 PmId [12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002) [19058170] Natural-product sugar biosynthesis and enzymatic glycodiversification. (Angew Chem Int Ed Engl. , 2008) [24911465] A general NMR-based strategy for the in situ characterization of sugar-nucleotide-dependent biosynthetic pathways. (Org Lett. , 2014) comment [PMID:12183629] calicheamicin生合成遺伝子クラスターの報告このORFは、Fig2の生合成 clusterにて、expected production of four unusual activated nucleotide sugars (S1 to S14)として示されている。本文中ふれていない。 --- [PMID:19058170] 天然糖の生合成に関する総論この論文中で3-methoxy-L-rhamnoseの生合成経路に関与すると示している。 --- [PMID: 24911465](2014) 反応物と産物の検出法の有用性を確認するため、calicheamicin生合成に関連したsugar nucleotide生合成酵素4つ・dTDP-glucose-4,6-dehydratase CalS3 ・dTDP-glucose-4-aminotransferase CalS13 ・dTDP-glucose-3,5-epimerase CalS1 ・dTDP-glucose-4-ketoreductase CalS2 の特徴づけをしている。 CalS1とCalS2と一緒に加えると望まれた産物をもたらしたが、どちらを欠いても単独では産物を得られない。
Related Reference	ACC Q9L9E9 PmId [15752721] Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides. (Arch Biochem Biophys. , 2005) comment Streptomyces sphaeroides_novS NovS NovW, novU, and novSの機能解析。 NovW, novU, and novSを大腸菌にて発現。 NovSはdTDP-L-rhamnoseに帰着するためにNADH存在下でdTDP-4-keto-6-deoxy-d-glucoseと一緒に NovWの反応から形成された産物を還元することが示された。
Related Reference	ACC Q93F84 PmId [12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002) comment Aneurinibacillus thermoaerophilus_rmlD dTDP-dehydrorhamnose reductase (EC 1.1.1.133) Aneurinibacillus thermoaerophilusのdTDP-4-dehydrorhamnose reductase (RmlD)をE. coliで発現させ、活性等の諸性質について調べている。

Sequence

>NDP-hexose C-4 ketoreductase [CalS2]
MSVPTPRLLVTGAGGLLGRELGELLAGSGDRHTTLDRSALDITDAAAVRHAVPGHDVVVN
AAAWTDVDGAESDEAGATEVNGAGPANLARACARTGALLIHLSSDYVFSGETTVPWPEDA
PPAPLNAYGRSKLAGERAVRGLLPAGGYVVRTGWLYGRHGPNFVRAVLRRAAETEFVDVV
DDQHGQPTWARALAGRLVALAGLAAAGRAPAGIYHGTAAGRTTWYGFARTVFALHGLDPD
RVRPTGSGTLAARPARRPRISVLGHDGWARAGLGPLPPWRRMIEAAVAEERSRAGPAGQD
RTRRRSA

>NDP-hexose C-4 ketoreductase [CalS2]
ATGAGCGTGCCGACGCCACGGTTGCTGGTGACCGGCGCGGGCGGGTTGCTGGGCCGCGAG
TTGGGCGAGCTCCTCGCCGGCAGCGGCGACCGGCACACCACCCTCGACCGGTCCGCGCTC
GACATCACCGACGCCGCCGCGGTGCGGCACGCCGTGCCCGGCCACGACGTCGTCGTCAAC
GCCGCCGCCTGGACCGACGTCGACGGGGCCGAGTCGGACGAGGCCGGCGCCACCGAAGTG
AACGGCGCCGGCCCGGCCAACCTGGCCCGGGCGTGTGCGAGGACCGGGGCGCTCCTGATC
CACCTGTCCAGCGACTACGTCTTCTCCGGCGAGACCACGGTGCCGTGGCCGGAGGACGCC
CCGCCGGCCCCGTTGAACGCGTACGGCCGTAGCAAGCTCGCCGGCGAGCGGGCCGTGCGC
GGCCTGCTGCCCGCCGGGGGCTACGTGGTCCGGACCGGGTGGCTGTACGGCCGGCACGGC
CCGAACTTCGTCCGCGCGGTGCTGCGCCGGGCCGCCGAGACGGAGTTCGTGGACGTCGTG
GACGACCAGCACGGCCAGCCCACCTGGGCGCGCGCGCTCGCCGGCCGGCTGGTCGCGCTG
GCCGGGCTGGCCGCCGCCGGGCGGGCGCCGGCCGGGATCTACCACGGCACGGCGGCCGGT
CGGACCACCTGGTACGGCTTCGCCCGCACGGTCTTCGCGCTGCACGGGCTCGACCCGGAC
CGCGTGCGCCCGACGGGCAGCGGGACGCTGGCCGCCCGGCCCGCCCGGCGGCCACGGATC
TCCGTCCTCGGGCACGACGGGTGGGCGCGGGCCGGCCTCGGCCCGCTGCCGCCGTGGCGA
CGGATGATCGAGGCGGCCGTGGCCGAGGAGCGGAGCCGGGCCGGGCCGGCCGGCCAGGAC
CGCACGCGACGGAGGTCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005913 dTDP-4-dehydrorhamnose reductase (Family) TIGR01214 RmlD PF04321 RmlD_sub_bind IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Calm_00100 Calm_00120