Calm_00270 Calm_00270   Calm_00290 Calm_00290

Calm_00280 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction32,797 / 31,589 / - [in whole cluster]
32,797 / 31,589 / - [in contig]
Locationcomplement(31589..32797) [in whole cluster]
complement(31589..32797) [in contig]
TypeCDS
Length1,209 bp (402 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative aminotransferase
Product (GenBank)CalE4
Gene
Gene (GenBank)calE4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

polyketide construction or modificationに関与するORFとしている。機能解析はされていない。
Related Reference
ACC
P77444
PmId
[10739946] Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions. (J Biochem. , 2000)
[12941942] The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli. (J Biol Chem. , 2003)
comment
Escherichia coli (strain K12)_sufS
Cysteine desulfurase
EC2.8.1.7
[41%, 1e-86]

SufABCDSE operonでSufS-SufE sulfur transfer system を担うタンパク。

[PMID:10739946]
L-cysteine desulfurase/L-selenocysteine lyase。Cys=>SH+Ala/Se-Cys=>Se+Ala。活性 Se-Cys>Cys(in vitro)。
active site(364Cys)は基質Cysに対する活性に必須(mutantは基質Se-Cys活性減少するだけ)。これより、基質Cys,Se-Cysそれぞれの反応メカニズムが異なるかもと考察されている。

[PMID:12941942]
scaffold protein SufA(P77667)にSを供給(Fe-S clusterになる)。このSufSのみでは活性低いが、SufE(P76194)と結合すると活性上昇(基質Cysに対してのみ)。また、 SufBCD complex(P77522,P77499,P77689)によっても活性上昇。
Family/Domain
FD
IPR000192
comment
[IPR000192] Aminotransferase, class V/Cysteine desulfurase (Domain)

close this sectionSequence

selected fasta
>putative aminotransferase [CalE4]
MAGHGPPLAYLDNAATTQKPRSVLDAVVDFYTTANSNIGRGFYELSRRATGRYEEAREVV
RRFVNAAHADEIVFTRGTTDAVNLLADTFAERIVGAGDDVVVTGMEHNSNLLPWRRLCER
RGARLRIVPSPAGGPVRPEDLAAVLGRRTRLVAVSHVSNVLGTVNPVRELVRVAHDRGVP
VVVDGAQAVAHLPVDVREIGADFYCFSGHKVYAPMGSGVLYGRRELLADLPPYQVGGGTV
KGVSHDAPVSYVPVPARLEAGTPDIGAAVGLAAALTWLDGLDREAVRAHDADLVRYAVDV
LCDVDRVRVVGDPAADPAGIVSIAVDGLHPYDVGGHLDAHGIAVRSGVHCASAFLDDLGL
LGTVRLSFAVYSTRGEVDRVAEALRTVRAGEWTTRYPDIRFR
selected fasta
>putative aminotransferase [CalE4]
GTGGCCGGGCACGGCCCGCCGCTGGCCTACCTCGACAACGCCGCGACGACGCAGAAGCCC
CGGTCGGTCCTGGACGCGGTGGTCGACTTCTACACCACCGCCAACAGCAACATCGGGCGC
GGCTTCTACGAGCTCAGCCGCCGGGCCACCGGCCGCTACGAGGAGGCCCGGGAGGTGGTC
CGGCGGTTCGTGAACGCCGCCCACGCCGACGAGATCGTCTTCACCCGGGGCACGACGGAC
GCGGTGAACCTGCTCGCCGACACCTTCGCCGAGCGGATCGTCGGCGCCGGTGACGACGTC
GTGGTCACCGGCATGGAGCACAACTCCAACCTGCTGCCGTGGCGGCGGCTCTGCGAGCGG
CGCGGGGCGAGGCTGCGGATCGTGCCGTCGCCGGCCGGCGGGCCGGTCCGGCCGGAGGAC
CTGGCCGCCGTCCTGGGCCGCCGGACCCGCCTCGTCGCGGTCAGCCACGTCTCCAACGTG
CTCGGCACCGTCAACCCGGTGCGGGAGCTGGTCCGGGTGGCGCACGACCGGGGCGTGCCC
GTGGTCGTCGACGGCGCGCAGGCGGTCGCGCACCTGCCGGTCGACGTGCGGGAGATCGGC
GCGGACTTCTACTGCTTCTCCGGCCACAAGGTGTACGCCCCGATGGGCTCCGGGGTGCTC
TACGGCCGGCGCGAGCTGCTCGCCGACCTGCCGCCGTACCAGGTGGGCGGCGGGACGGTC
AAAGGGGTCTCGCACGACGCTCCGGTGTCCTACGTGCCGGTTCCGGCGCGGCTGGAGGCC
GGCACCCCGGACATCGGCGCCGCGGTCGGGCTCGCCGCCGCGTTGACCTGGCTGGACGGG
CTCGACCGGGAGGCGGTGCGGGCACACGACGCGGACCTCGTCCGGTACGCCGTCGACGTC
CTGTGCGACGTCGACCGGGTGCGGGTCGTCGGCGACCCGGCCGCCGACCCGGCCGGCATC
GTCTCCATCGCCGTGGACGGCCTGCACCCGTACGACGTGGGCGGGCACCTCGACGCGCAC
GGCATCGCCGTACGGTCCGGCGTGCACTGCGCCAGCGCGTTCCTCGACGACCTGGGTCTG
CTCGGCACGGTGCGGCTCTCGTTCGCGGTCTACAGCACCCGTGGCGAGGTCGACCGGGTC
GCCGAGGCGCTGCGCACCGTGCGGGCGGGGGAGTGGACCACCCGGTACCCCGACATCCGG
TTCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000192 Aminotransferase, class V/Cysteine desulfurase (Domain)
[10-380] 1.69999999999998e-121 PF00266
PF00266   Aminotran_5
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[17-273] 2.70000000000002e-81 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[274-380] 3.7e-14 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[4-389] 2.1000026783403e-120 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Calm_00270 Calm_00270   Calm_00290 Calm_00290
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