Dynm_00200 Dynm_00200   Dynm_00220 Dynm_00220

Dynm_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction34,326 / 33,652 / - [in whole cluster]
34,326 / 33,652 / - [in contig]
Locationcomplement(33652..34326) [in whole cluster]
complement(33652..34326) [in contig]
TypeCDS
Length675 bp (224 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative HxlR family transcriptional regulator
Product (GenBank)DynU8
GenedynU8
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • putative transcriptional regulator; CalU8-like protein
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Dynemicin生合成遺伝子クラスターの報告。

dynU8: Putative transcriptional regulator

機能解析はされていない。
Related Reference
ACC
Q8GMD6
NITE
C1027_00570
PmId
[21250756] Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. (J Nat Prod. , 2011)
comment
Streptomyces globisporus
Putative uncharacterized protein

[PMID:21250756]
SgcR, SgcEの機能解析

putative DNA-binding proteinsと推定されるsgcE1,sgcRの不活性化株作製。sgcE1はC-1027の生合成に影響を与えなかったがsgcR不活性化株はC-1027とheptaeneのtitersが著しく増加した。S.globisporus wild-typeとsgcE1の不活性化株間でassayに変化は見られないことから、SgcE1はC-1027 biosynthesisに必須ではないことを示した。enediyne core biosynthesis geneだけでなくregulatory geneでもないことを示した。sgcE1の機能は明らかになっていない。
Family/Domain
FD
IPR002577
comment
[IPR002577] Helix-turn-helix, HxlR type (Domain)

close this sectionSequence

selected fasta
>putative HxlR family transcriptional regulator [DynU8]
MGLLKDRRTYDQQCGLAYALDVLGERWTLLIVRELLIRPRRYGELLDALQGIGTNLLADR
LGFLVEAGLVRPLDPQRRTAGYALTELGERMREPVLSLARFGLTYAAHQPPPAASVTRPA
WAILATDAMLDDTRAPGIDEVYGFDVDGEQFHVVVEGGRAVLHPTAAPDATLHVKTDAKT
FFQLGVRRLDPLEAVLAGSVQVSGPPAAMSRCLRLLGLAGNPLG
selected fasta
>putative HxlR family transcriptional regulator [DynU8]
ATGGGGCTGCTGAAGGACCGACGCACCTACGACCAACAGTGCGGACTGGCCTATGCGCTG
GACGTTCTCGGCGAACGCTGGACTTTGCTGATCGTCCGGGAGCTGCTGATCCGGCCCCGT
CGCTACGGCGAACTCCTCGATGCTTTGCAAGGAATCGGCACCAACCTTCTGGCCGACCGG
CTCGGCTTCCTCGTCGAAGCGGGGCTGGTCCGGCCGCTCGATCCGCAGCGGCGGACCGCG
GGCTACGCGCTGACCGAGTTGGGTGAGCGGATGCGGGAGCCGGTGCTGAGCCTGGCCCGG
TTCGGCCTGACCTACGCCGCCCACCAGCCGCCGCCGGCCGCTTCGGTGACCCGGCCGGCC
TGGGCGATCCTCGCCACCGACGCGATGCTCGACGACACCCGCGCCCCCGGGATCGACGAG
GTCTACGGCTTCGACGTCGACGGCGAGCAGTTCCACGTCGTGGTGGAGGGCGGCCGGGCG
GTCCTGCACCCCACCGCCGCGCCCGACGCGACACTCCACGTGAAGACCGACGCGAAGACC
TTCTTCCAGCTCGGGGTGCGGCGGCTGGACCCGCTGGAGGCGGTGCTCGCCGGCTCGGTG
CAGGTCTCCGGGCCGCCGGCCGCCATGTCGCGCTGCCTGCGGCTGCTCGGCCTGGCCGGC
AACCCGCTCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002577 Helix-turn-helix, HxlR type (Domain)
[22-108] 6e-13 PF01638
PF01638   HxlR
[14-110] PS51118
PS51118   HTH_HXLR
IPR003033 SCP2 sterol-binding domain (Domain)
[108-217] 6.59999852526375e-10 SSF55718
SSF55718   SCP2
[133-210] 5.5e-07 PF02036
PF02036   SCP2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[13-97] 2.2e-06 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP No significant hit
TMHMM No significant hit
Dynm_00200 Dynm_00200   Dynm_00220 Dynm_00220
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