Dynm_00420 Dynm_00420   Dynm_00440 Dynm_00440

Dynm_00430 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction57,064 / 56,720 / - [in whole cluster]
57,064 / 56,720 / - [in contig]
Locationcomplement(56720..57064) [in whole cluster]
complement(56720..57064) [in contig]
TypeCDS
Length345 bp (114 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative ArsR family transcriptional regulator
Product (GenBank)putative transcriptional regulator
GenedynR8
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF32; ArsR family
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Dynemicin生合成遺伝子クラスターの報告。

dynR8 Putative transcriptional regulator

機能解析はされていない。
Related Reference
ACC
Q6PPB8
PmId
comment
Streptomyces griseus
Putative transcription regulator
sgtR2

sgtR1,sgtR2の機能解析

three ORFs on the opposite strand were named ORF2 (SgtR1), ORF3 (SgtR2), and ORF4 (GenBankAccession No. AAS92596).
Nucleotide sequence:GenBank Accession No. AY588948.

sgtR2 は、(ArsR)-type helix-helix (HTH) domainを持つputative transcriptional regulatorと推測。クローニングし、トリプシン活性や転写解析を調べている。S. lividansにおけるsprTの発現データと、total RNA解析はSgtR1 or SgtR2がsprTの過剰発現を引き起こすtranscriptional regulatorであると推測された。sgtR1 and sgtR2 genesはAdpAに依存しない事から、AdpA regulonのメンバーではないと推定している。
Family/Domain
FD
IPR001845
comment
[IPR001845] HTH ArsR-type DNA-binding domain (Domain)

close this sectionSequence

selected fasta
>putative ArsR family transcriptional regulator [putative transcriptional regulator]
MSPATGVDVFAALASPVRRDLIRLLRDGGPQSVQDLAASFDMRRPSVSEHLKVLRDAGLV
TERRSGRKRLYRLEPEPLMDLRDWLTPYERFWRERMSDFAALVAEESATEGKPV
selected fasta
>putative ArsR family transcriptional regulator [putative transcriptional regulator]
ATGTCCCCCGCCACGGGAGTGGACGTCTTCGCGGCCCTGGCCAGCCCGGTGCGGCGCGAC
CTCATCCGGCTGCTGCGCGACGGCGGCCCGCAGTCGGTGCAGGACCTGGCCGCCTCGTTC
GACATGCGCCGGCCCAGCGTCTCCGAGCACCTCAAGGTGCTCCGCGACGCCGGCCTGGTG
ACCGAGCGCCGCAGCGGCCGGAAGCGGCTCTACCGGCTGGAGCCGGAGCCGCTCATGGAC
CTGCGCGACTGGCTCACCCCCTACGAGCGGTTCTGGCGTGAGCGGATGAGCGACTTCGCC
GCGCTGGTCGCCGAGGAGTCCGCGACCGAGGGGAAGCCGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001845 HTH ArsR-type DNA-binding domain (Domain)
[8-87] 1.20000117458134e-27 SM00418
SM00418   HTH_ARSR
[10-25] 2.79999889404114e-12 PR00778 [43-58] 2.79999889404114e-12 PR00778 [58-73] 2.79999889404114e-12 PR00778
PR00778   HTHARSR
[1-93] PS50987
PS50987   HTH_ARSR_2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[7-106] 9.40000000000003e-34 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP No significant hit
TMHMM No significant hit
Dynm_00420 Dynm_00420   Dynm_00440 Dynm_00440
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