Dynm_00590 : CDS information

Location

Organism	Micromonospora chersina
Strain	ATCC 53710 (=NBRC 15963)
Entry name	Dynemicin
Contig	EF552206
Start / Stop / Direction	74,911 / 71,495 / - [in whole cluster] 74,911 / 71,495 / - [in contig]
Location	complement(71495..74911) [in whole cluster] complement(71495..74911) [in contig]
Type	CDS
Length	3,417 bp (1,138 aa)

Annotation

Category	5.1 general function
Product	putative restriction-modification system restriction subunit putative restriction enzyme R protein
Product (GenBank)	type I site-specific deoxyribonuclease protein R
Gene	orf27
Gene (GenBank)
EC number	3.1.21.-
Keyword
Note
Note (GenBank)	ORF44
Reference	ACC B2BM74 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Dynemicin生合成遺伝子クラスターの報告。 orf27 Putative restriction enzyme 機能解析はされていない。 GenBank EF552206ではcomplementと表示あるが、cluster論文Fig.3で示されているORFの向きはそれとは逆になっている。
Related Reference	ACC P08956 PmId [3323532] ( , ) comment Escherichia coli_hsdR Type I restriction enzyme EcoKI R protein [26%, 4e-47] hsd genes(hsdS, R, M)のcloning、転写解析をしている。R polypeptideは、様々なATPasesと相同性を示した。
Family/Domain	FD IPR007409 comment [IPR007409] Restriction endonuclease, type I, HsdR, N-terminal (Domain)

Sequence

>putative restriction-modification system restriction subunit [type I site-specific deoxyribonuclease protein R]
MSNFAFLRAEWPVLFGEALRAERLAVADPRASCFYARRSLELALTWLFEADSTLNKPYKD
DLSAMIHEPSLRNLVGNSLQAKMNIIRKQGNTAVHKSTPVTPKEAVPVVRELFHVMYWIA
RHYTRDQAKVPASALAFNPDLIPRPVPSQVRQQTQAELKALADKLAAQDAALAAEREKSA
TLDAELAKLRAEIAAAKAANEARPDDHDYDEAQTRDLFIDLMLREAGWRLDRPEDREFEV
TGMPNGTGTGYVDYVLWGDDGKPLAVVEAKRTRRDATVGQQQAKLYADCLEQRYGQRPVI
FYTNGYETWLWDDTAYPPRPVQGFYTKDELHLLIQRRDTRKPLADVPINLEIVERYYQHR
AIRAIGEAFETQRQRQALVVMATGAGKTRTVIALVDLLMRANWVKRVLFLADRNALVNQA
VNAFKAHLPDAATVNLVTEKNTEGRVYVSTYPTMMGLINETASGERRFGPGYFDLVIIDE
AHRSVYQKYRAIFSWFDSLLVGLTATPRNEIDRNTYSLFNLEDGVPTDHYDLDDAVNEGF
LVPPRAVSVPLKFQRDGIRYDDLTEEEKDDWDAIDWGEDGPPDSVDPDAVNKWLFNADTV
DKVLETLMTHGHHVAGGDRIGKTIIFAKNNAHADFIQKRFDLAYPMHRGHLARVITHKTE
YAQSLIDAFSEKEKDPHIAVSVDMLDTGIDVPEVVNLVFFKIVRSKSKFWQMIGRGTRLC
KDLYGPDQHKQDFFIFDFCQNFEFFNHNPKAAEGTLGESLTERLFKAQLELIYRLDQRIP
CDAGAETEADGTQSEAALRWDLARNLHRRVENIHLDNFVVRPKRRLVQYYVDFEHWHRLT
PEAVDEIGSNLAGLPTAVKDEDEAAKRFDLLVLRLQLGQLDVEPAYDRLRRQVQDIASTL
LEQTSIPAVREQQQLLDEVAGDEWWQDITLPMLELMRRRLRGLVKLIERSKRAIVYTDFT
DELGEISVVTLNDIKIGTEFERFRAKARVYLRAHEDHLALQKLRRNKQLSPTDLDELERM
LAASGGGTEDIDEARRSAHGLGLFIRSLVGLDREAATEAFGEFLAGRTLTANQINFVNLI
ITHLTQNGVMEPARLYESPFSDIAPHGPDSIFPSADVDHLITILNSVRDTAAPAAEVA

>putative restriction-modification system restriction subunit [type I site-specific deoxyribonuclease protein R]
ATGAGCAACTTCGCGTTCCTGCGGGCTGAGTGGCCCGTGCTGTTTGGCGAGGCCCTGCGG
GCGGAGCGGCTGGCCGTCGCTGATCCGCGGGCCTCGTGCTTCTACGCCCGGCGGTCGCTC
GAACTCGCGCTTACCTGGCTCTTCGAGGCTGACAGCACGCTGAACAAGCCGTACAAGGAC
GACCTGTCGGCGATGATCCACGAGCCGTCGCTGCGCAACCTGGTGGGCAACAGCCTGCAG
GCGAAGATGAACATCATCCGCAAGCAGGGCAACACGGCCGTCCACAAGAGCACGCCGGTG
ACGCCGAAGGAGGCGGTGCCGGTCGTCCGCGAGCTGTTCCACGTCATGTACTGGATCGCC
CGGCACTACACCCGCGACCAGGCCAAGGTGCCGGCCAGCGCGCTCGCCTTCAACCCGGAC
CTCATCCCCCGGCCGGTTCCCTCGCAGGTCCGGCAGCAGACCCAGGCTGAGCTCAAGGCG
CTGGCTGACAAGCTCGCCGCCCAGGATGCGGCGCTGGCGGCCGAGCGGGAGAAGTCCGCG
ACCCTCGACGCTGAGTTGGCGAAGCTGCGGGCCGAGATCGCCGCCGCGAAGGCCGCCAAC
GAGGCCCGCCCCGACGACCACGACTACGACGAGGCGCAGACCCGCGACCTCTTCATCGAC
CTCATGCTGCGGGAGGCCGGCTGGCGCCTCGACCGCCCGGAGGACCGCGAGTTCGAGGTC
ACCGGCATGCCCAATGGCACGGGCACCGGGTACGTCGACTACGTCCTGTGGGGTGACGAC
GGCAAGCCGCTCGCGGTGGTCGAGGCCAAGCGCACCCGCCGGGACGCCACCGTCGGTCAG
CAGCAGGCCAAGCTGTACGCCGACTGCCTGGAGCAGCGGTACGGCCAGCGGCCGGTCATC
TTCTACACCAACGGCTACGAGACCTGGCTCTGGGACGACACCGCCTACCCTCCCCGCCCG
GTGCAGGGCTTCTACACCAAGGATGAGCTCCACCTGCTGATCCAGCGCCGCGACACCCGC
AAGCCGCTCGCGGACGTGCCCATCAACCTGGAGATCGTCGAGCGGTACTACCAGCACCGG
GCGATCCGGGCCATCGGCGAGGCGTTCGAGACGCAGCGGCAGCGCCAGGCGCTTGTCGTG
ATGGCCACCGGCGCCGGCAAGACCCGCACCGTGATTGCCCTGGTCGATCTGCTGATGCGC
GCGAACTGGGTCAAGCGGGTGCTCTTCCTCGCCGACCGCAACGCGCTGGTTAACCAGGCG
GTCAACGCGTTCAAGGCGCACCTGCCGGACGCGGCGACGGTCAACCTGGTCACCGAGAAG
AACACCGAGGGTCGGGTGTACGTCTCGACGTACCCGACCATGATGGGCCTGATCAACGAG
ACCGCGAGCGGTGAGCGTCGCTTCGGCCCCGGCTACTTCGACCTGGTCATCATCGACGAG
GCGCACCGGTCGGTCTACCAGAAGTACCGGGCGATCTTCTCCTGGTTCGACAGCCTCCTC
GTCGGGCTCACCGCGACCCCGCGCAACGAGATCGACCGCAACACCTACAGCCTGTTCAAC
CTGGAAGACGGCGTTCCCACCGACCACTACGACCTCGACGACGCGGTCAACGAGGGCTTC
CTTGTGCCGCCGCGGGCGGTCTCCGTACCGTTGAAGTTCCAGCGCGACGGCATCCGGTAC
GACGACCTCACCGAGGAGGAGAAGGACGACTGGGACGCCATCGACTGGGGTGAGGACGGA
CCGCCGGACAGCGTCGACCCGGACGCGGTCAACAAGTGGCTGTTTAACGCCGACACCGTG
GACAAGGTGCTGGAGACGTTGATGACCCACGGCCATCACGTCGCGGGCGGCGACCGGATC
GGCAAGACCATCATCTTTGCCAAGAACAACGCCCACGCGGACTTCATCCAGAAGCGGTTC
GACCTCGCGTACCCGATGCACCGAGGCCACCTGGCCCGGGTCATCACCCACAAGACCGAG
TACGCCCAGAGCCTCATCGACGCCTTCTCGGAGAAGGAGAAGGACCCGCACATCGCGGTC
TCGGTGGACATGCTCGACACCGGCATCGACGTGCCCGAGGTCGTCAACCTGGTCTTCTTC
AAGATCGTGCGGTCGAAGTCGAAGTTCTGGCAGATGATCGGCCGCGGCACCCGTCTCTGC
AAGGACCTCTACGGTCCCGACCAGCACAAGCAGGACTTCTTCATCTTCGACTTCTGCCAG
AACTTCGAGTTCTTCAACCACAACCCGAAGGCGGCCGAGGGCACGCTGGGCGAGTCGCTG
ACCGAGCGGCTGTTCAAGGCCCAGCTCGAACTGATCTACCGCCTCGACCAGCGAATCCCC
TGCGACGCCGGCGCCGAGACGGAGGCCGACGGGACGCAGAGCGAGGCGGCGCTGCGCTGG
GACCTCGCCCGCAACCTGCACCGCCGGGTGGAGAACATCCACCTCGACAACTTCGTCGTA
CGCCCCAAGCGGCGCCTGGTGCAGTACTACGTCGACTTCGAGCACTGGCACCGGCTCACC
CCCGAGGCGGTGGACGAGATCGGCAGCAACCTCGCCGGCCTGCCCACCGCCGTGAAGGAC
GAGGACGAGGCCGCCAAGCGGTTCGACCTGCTGGTGCTGCGCCTCCAGCTCGGCCAGCTC
GACGTGGAACCCGCCTACGACCGGCTGCGCCGGCAGGTGCAGGACATCGCCTCGACGCTG
CTGGAGCAGACCAGCATCCCGGCGGTCCGCGAGCAGCAGCAACTCCTCGACGAGGTGGCC
GGCGACGAGTGGTGGCAGGACATCACCCTGCCGATGCTGGAGCTGATGCGCCGCCGTCTC
CGTGGGCTGGTCAAGCTGATCGAGAGGTCCAAGCGGGCCATCGTCTACACCGACTTCACC
GACGAGCTGGGCGAGATCTCCGTCGTCACGTTGAATGACATCAAGATCGGCACGGAGTTC
GAGCGGTTCCGCGCGAAGGCCCGGGTGTACCTGCGCGCTCACGAGGATCACCTGGCGTTG
CAGAAGCTGCGCCGCAACAAGCAGCTTTCCCCGACCGACCTCGATGAGCTGGAGCGGATG
CTGGCGGCCAGCGGCGGCGGCACGGAGGACATCGATGAGGCCCGCCGCTCGGCCCACGGG
CTCGGCCTGTTCATCCGCTCGCTGGTCGGACTCGACAGGGAAGCGGCCACCGAGGCGTTC
GGCGAGTTCCTGGCCGGCCGGACCCTGACCGCGAACCAGATCAACTTCGTCAACCTGATC
ATCACCCACCTCACGCAGAACGGCGTGATGGAGCCCGCGCGGCTCTACGAATCCCCGTTC
AGCGACATCGCCCCGCACGGGCCGGACTCGATCTTCCCGTCCGCCGACGTCGACCACCTG
ATCACGATCCTCAACTCCGTCCGAGACACCGCCGCCCCTGCCGCCGAGGTGGCTTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001650 Helicase, C-terminal (Domain) PS51194 HELICASE_CTER PF00271 Helicase_C IPR006935 Helicase/UvrB domain (Domain) PF04851 ResIII IPR007409 Restriction endonuclease, type I, HsdR, N-terminal (Domain) PF04313 HSDR_N IPR013670 EcoEI R protein C-terminal domain (Domain) PF08463 EcoEI_R_C IPR014001 Helicase, superfamily 1/2, ATP-binding domain (Domain) PS51192 HELICASE_ATP_BIND_1 SM00487 DEXDc IPR025285 Domain of unknown function DUF4145 (Domain) PF13643 DUF4145
SignalP	No significant hit
TMHMM	No significant hit

Dynm_00580 last last