Gelda2_00010 : CDS information

Location

Organism	Streptomyces geldanamycininus
Strain	NRRL 3602 (=NBRC 14620)
Entry name	Geldanamycin
Contig	AY179507
Start / Stop / Direction	11,984 / 11,301 / - [in whole cluster] 684 / 1 / - [in contig]
Location	complement(11301..11984) [in whole cluster] complement(1..684) [in contig]
Type	CDS
Length	684 bp (227 aa)

Annotation

Category	4.3 transport
Product	putative amino acid exporter
Product (GenBank)	similar to predicted RhtB family transporters
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF16
Reference	ACC Q84G31 PmId [12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003) comment ansamycin系抗生物質geldanamycin産生のgene clusterのクローニング、及び配列解析と遺伝子破壊による特性解析についての報告。 ORF16(227aa) : Efflux ORF16 と ORF17 はputative geldanamycin resistance and/or export genesの候補である。 ORF16 に関する機能実験無し。
Related Reference	ACC C6GYU4 NITE Pimar_00010 PmId [19505319] PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. (Microb Cell Fact. , 2009) comment Blast id87%, 1e-102 Streptomyces natalensis_pimT PimT protein pimaricin gene clusterに隣接するRhtB family amino-acid exporter PimTについての論文。 pimTのgene replacement mutantは、L-homoserine, L-serine, and L-homoserine lactoneへの感受性増加。相補で回復。 pimT発現はhomoserineとquorum-sensing pimaricin-inducer PI-factor(autoinducer)によって誘導される。 pimT deletion mutantはpimaricin産生を65%、PI産生を50%まで減らし、pimT相補、外因性PI-factorの添加でpimaricin産生は回復する。これはPimTがPI-factor exporterとして使用されることを示す。以上から、pimaricin産生はamino acid exporter PimTによるquorum sensing pimaricin-inducer PI-factorの分泌を介して調節されている。

Sequence

>putative amino acid exporter [similar to predicted RhtB family transporters]
MSPGRSDSGGGGHVVSTESVLTFAAMSLLVIVIPGPSVLFVVGRALAHGRRTALATVLGN
VIGCYALVIAVAWGLGALVESSVALFMGVKLAGAAYLVYLGVQAFRHRREMRVANMDAPA
DERRGDLRSILDGILVGVTNPKGIVFFAAVLPQFVDHSVGHVPVQMMVLGLVPITIGLVT
DTLWGLGASAARSWFARSDRRLSMVGGAGGLAMIGLGVTVAATGRAD

>putative amino acid exporter [similar to predicted RhtB family transporters]
ATGTCCCCTGGACGGAGCGATTCAGGAGGTGGCGGACACGTGGTGTCCACGGAGAGTGTT
CTCACGTTCGCGGCGATGTCGCTCTTGGTGATCGTGATCCCGGGGCCGAGTGTGCTGTTC
GTGGTCGGCAGGGCCCTCGCACACGGCCGCCGCACGGCGCTCGCGACGGTCCTCGGCAAT
GTGATCGGCTGCTACGCCCTGGTGATCGCCGTGGCCTGGGGCCTCGGCGCGCTGGTGGAG
AGCTCGGTGGCGCTGTTCATGGGCGTGAAGCTGGCGGGAGCGGCGTATCTCGTCTACCTG
GGTGTGCAGGCGTTCCGGCACCGCAGGGAGATGCGCGTGGCGAACATGGACGCTCCGGCC
GATGAGCGGCGTGGTGATCTGCGTTCGATTCTGGACGGCATTTTGGTGGGCGTCACCAAC
CCGAAGGGCATCGTCTTCTTCGCGGCGGTGCTACCGCAGTTCGTCGACCACTCGGTGGGT
CATGTTCCCGTCCAGATGATGGTGTTGGGCCTGGTCCCGATCACCATCGGCCTGGTCACG
GACACCCTGTGGGGCCTGGGCGCCTCGGCGGCCCGCTCCTGGTTCGCCCGCTCGGACCGC
CGCCTTTCGATGGTCGGCGGGGCGGGCGGCCTCGCGATGATCGGCCTGGGCGTGACGGTG
GCGGCGACTGGCCGGGCCGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001123 Lysine-type exporter protein (LYSE/YGGA) (Family) PF01810 LysE
SignalP	No significant hit
TMHMM	Transmembrane 6

Gelda2_00330 Gelda2_00020