Salino3_00110 Salino3_00110   Salino3_00130 Salino3_00130

Salino3_00120 : CDS information

close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction13,354 / 12,326 / - [in whole cluster]
13,354 / 12,326 / - [in contig]
Locationcomplement(12326..13354) [in whole cluster]
complement(12326..13354) [in contig]
TypeCDS
Length1,029 bp (342 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative acyltransferase
Product (GenBank)putative 3-oxoacyl-(acyl-carrier-protein ) synthase III
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • Orf12
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

Orf12 (342aa) : 3-Oxoacyl-(acyl-carrier-protein) synthase III

Lasalocid clusterの同定論文を引用し、butyrate伸長unitsの供給に関連すると述べている。
orf12を置換して不活化するとsalinomycin収量がwildの36%になった。
Related Reference
ACC
P68795
PmId
[11959561] Purification, characterization, and identification of novel inhibitors of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus. (Antimicrob Agents Chemother. , 2002)
comment
334th, id37%, 2e-57
Staphylococcus aureus_fabH
Beta-ketoacyl-ACP synthase III

fabH の遺伝子をクローニング、精製し、諸性質を調べている。FabHはhomodimeric structureで、malonyl-ACPに対してのKm値(1.76±0.40μM)と共に、acetyl-CoA, butyryl-CoA, isobutyryl-CoAに対して活性を持つことが示されている。
ACC
H1ZZT2
NITE
Salino_00900
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
配列は[H6D568]と全く同じ。株違い。

monC gene-based hybridisation probeを使ってsalinomycin(sal)生合成gene clusterを同定。

salQ (342aa) : putative 3-oxoacyl-(acyl carrier protein) synthase

配列解析のみ。
salinomycin生合成における役割は不明。

close this sectionSequence

selected fasta
>putative acyltransferase [putative 3-oxoacyl-(acyl-carrier-protein ) synthase III]
MTGIGILGTGSYLPADRVSNAVVAERAGVTEEWIARKTGIRERRYAADSEATSDLAVEAA
RAALDSAGLDAAQLSWILLATSTPDSPQPATACLVQDRIGAVNAAAFDLNSVCSGFVFGL
VTAARLLAAPGPDGPGYGLVIGADTYSRIIDREDRRTAILFGDGAGAAVLGPVRSGHGLI
GSQLSTVGGTEDLIRVEAGGSRLPASEKTVAEGLHHFRMNGRGVSEFVAAELPPAVREVI
ASGGLRNSDIDHFIPHQANGVMLAKLLPELGLDQARTHLTVERHGNTSAASIAITLDEAH
RSGAFGDGDVLLLTGFGGGMSLGSALVRWDGPAARAHRKETV
selected fasta
>putative acyltransferase [putative 3-oxoacyl-(acyl-carrier-protein ) synthase III]
ATGACAGGAATAGGAATACTGGGCACCGGGTCGTATCTTCCTGCTGACAGAGTTTCCAAT
GCCGTCGTCGCCGAACGGGCCGGGGTCACCGAGGAGTGGATCGCGCGCAAGACCGGTATC
CGGGAACGCCGTTACGCCGCCGATTCGGAGGCGACATCCGACCTCGCGGTGGAGGCGGCC
CGGGCCGCGCTCGACTCGGCGGGACTGGACGCCGCCCAGCTGTCCTGGATCCTCCTGGCG
ACCTCCACCCCGGACAGCCCGCAGCCGGCCACCGCCTGCCTCGTGCAGGACCGCATCGGC
GCGGTGAACGCGGCGGCCTTCGACCTCAACTCGGTGTGCAGCGGCTTCGTCTTCGGCCTC
GTCACCGCCGCCCGGCTGCTCGCCGCACCGGGACCGGACGGCCCGGGATACGGACTGGTC
ATCGGCGCCGACACCTACTCCCGGATCATCGACCGCGAGGACCGGCGCACCGCGATCCTC
TTCGGCGACGGGGCGGGCGCCGCGGTCCTCGGACCGGTCAGATCCGGCCACGGCCTGATC
GGCTCGCAGCTCTCCACGGTGGGCGGCACCGAGGACCTCATCCGGGTCGAGGCGGGCGGC
AGCCGGCTGCCCGCCTCCGAGAAGACCGTCGCCGAGGGCCTGCACCACTTCCGTATGAAC
GGCCGCGGGGTGAGCGAATTCGTCGCCGCCGAACTGCCGCCCGCCGTACGGGAGGTGATC
GCCTCCGGCGGTCTGCGGAACTCCGACATCGACCACTTCATTCCGCATCAGGCCAACGGC
GTGATGCTCGCGAAGCTCCTGCCCGAACTCGGTCTCGACCAAGCGCGCACCCATCTGACC
GTGGAACGGCACGGCAACACCAGCGCCGCCTCCATCGCGATCACCCTCGACGAGGCGCAC
CGCTCGGGTGCCTTCGGCGACGGGGACGTGCTGCTGCTCACCGGGTTCGGCGGCGGTATG
TCGCTCGGCTCGGCGCTCGTGCGCTGGGACGGTCCGGCCGCCCGCGCCCATCGAAAGGAA
ACCGTATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004655 Beta-ketoacyl-acyl carrier protein synthase III (FabH) (Family)
[2-329] 33.994 MF_01815
MF_01815   FabH
[5-329] 1e-89 TIGR00747
TIGR00747   fabH: 3-oxoacyl-[acyl-carrier-protein] synthase 3
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)
[242-329] 6.2e-29 PF08541
PF08541   ACP_syn_III_C
IPR013751 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (Domain)
[107-187] 3.3e-22 PF08545
PF08545   ACP_syn_III
IPR016038 Thiolase-like, subgroup (Domain)
[2-163] 3.9e-49 G3DSA:3.40.47.10 [164-329] 3.3e-41 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
[1-329] 9.1e-51 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Salino3_00110 Salino3_00110   Salino3_00130 Salino3_00130
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