Salino3_00150 Salino3_00150   Salino3_00170 Salino3_00170

Salino3_00160 : CDS information

close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction48,450 / 53,378 / + [in whole cluster]
48,450 / 53,378 / + [in contig]
Location48450..53378 [in whole cluster]
48450..53378 [in contig]
TypeCDS
Length4,929 bp (1,642 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase
Gene
Gene (GenBank)slnA4
EC number
Keyword
Note
Note (GenBank)
  • SlnA4
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

SlnA4 (1,642aa) : PKS
Module 7 : KS-ATp-KRa-ACP

KRa = inactive domain
Related Reference
ACC
H1ZZT6
NITE
Salino_00940
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
配列は[H6D572]と全く同じ。株違い。

monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

salAIV (1642aa): polyketide synthase
KS, AT, KR, ACP

module 7のKR domainはactive-site residues Tyr150 and Asn154がそれぞれPhe and Serによって置換されているので実際にはinactiveであるようだ。

close this sectionPKS/NRPS Module

7 methylmalonyl-CoA
KS34..410
AT575..893
kr1196..1373
ACP1485..1555

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase]
MDNEKKLLDHLKWVTAELRQARQKLREAEADEPEPIAIVSMACRYPGGVRSPEDLWQLVR
EGRDAITGFPTGRGWDLASLYDADPDRLGTSYVREGGFVHDAGDFDAEFFGISPREALAM
DPQQRLLLETSWEAFERAGVDPAAVAGSRTGVFVGTTYTGYGSDREGAEENVEGHLMTGI
ATAVASGRLSYTYGFEGPAVSLDTMCSSSLVALHLAVQALRQKECTLALAGGSQIMSTPD
VYVEFSRQRGLSPDGRCKPFAAAADGTGWSEGVGVLLLERLSDARRNGHRVLAVVRGSAI
NQDGASNGLTAPNGPSQQRVIEQALANARLSPHQVDLIEAHGTGTTLGDPIEAQALLATY
GGSRPEGRPLWLGSVKSNIGHAAAAAGVAGVIKAVMAVREGVLPKSLHIDAPTPEVDWSS
GAVALLTEEREWSVPGEEPRTAAISSFGASGTNAHVLVQYAPEPDPEPAAEAPAAVFTGA
ALPWLLSGRTAEGLRGQARSLHTYASTTATALPGAALGLATTRAALERRGAVLTSGTPGT
PDKDALLTGLDALAEGTPAAGILEGTTVSGADRPVFVFPGQGSQWAGMAVELLDSSSVFA
ARLGECAEALDPFVDWSLVDVLRQTEGAPGFDRVDVVQPALWAVMVSLAEVWRAAGVAPA
AVIGHSQGEIAAAAVSGALSLSDAAKVSALRARALLALAGKGGMVSVADAADSVRERISA
WGERLALASVNGPQSTVVSGDPEALDELMAGCEAEGVRARRINVDYASHGPQVEKIRTEV
LGALSGIEPRTAEVPFLSTVTGEFVTGTELDAEYWYRNLRNTVRFEDAVRQLLDRGHGAF
VEASAHPVLTVGVQETIDAAGAPAFVQGTLRREDGDAARFLASLAEAWTRGVPVNWAIVT
DSSAPPAEDLPTYAFQRRTYWLHGARTGSVQAPVDAVEAEFWDSVENGDIDSLSGSLALE
DSAPLAELLPALSSWRRRRRERGEVDSWRYRIDWQPLAESAPPALEGTWLLVTGDGVEAE
ILKTGESALSAHGATVHPLTLTGEGEREALVRQLLGAEVEHGPFAGVLSLLATAEPERGP
ATTLALVQALADAECEAPLWVATRGAVGTGPEEAPAHPAQAGAWGLGLVAALERPGGWGG
LVDLPAEPDENLAGRLAAALAGQEDQLALRATGTYVRRLARAPLPGSEVRPWEPADTVLV
TGATAPVGARTALLLAASGAKRLLLTVPGGEEQPAALVGELEEAGVQVTLAEWDGRDVTA
LRSLAEAAAADGAPVRGVFHAATRADLAPLDETTAADLAAATAAKTVPARALDEAFGEEV
EAFVLFSSVTSYWGGGEHAAFAAASAELDALAARRRSRGLAATSVAWGVWDLFDAEQNPA
EAAELQARSADRGLPLLDPETAWQALRLSLGRQETAIAVADVDWERFWPLFTSARPAPLL
SDLPEVRGLGRGLAEDTGTGADPGAAEALRSKLAGLSPAEQDRALTDLVCAHAAAVLGHS
SAGAVDAERAFKDLGFDSLTAVGLRNGLGAATGLSLPATLVFDYPTPAAMAGYVRDHLLA
GARQEATAAGVQSGLDQLEADLLSVALDKDERKNLTRRLEGLLSRFKDAQAAADEESVSG
KLDSASDEEIFAFIREEFGRPE
selected fasta
>polyketide synthase [polyketide synthase]
ATGGACAACGAAAAGAAGCTTCTCGATCACCTCAAGTGGGTGACGGCCGAACTCCGCCAG
GCCCGGCAGAAGCTGCGCGAGGCCGAGGCGGACGAGCCCGAGCCGATCGCCATCGTCTCC
ATGGCCTGCCGCTACCCCGGTGGCGTGCGCTCGCCCGAGGACCTGTGGCAGCTGGTCCGC
GAGGGCCGGGACGCCATCACCGGCTTCCCCACCGGCCGCGGCTGGGACCTGGCCTCGCTC
TACGACGCCGACCCGGACCGGCTCGGCACCAGCTACGTACGCGAGGGCGGCTTCGTCCAC
GACGCGGGCGACTTCGACGCCGAGTTCTTCGGCATCTCCCCGCGTGAGGCGCTTGCCATG
GACCCGCAGCAGCGGCTCCTCCTGGAAACCTCCTGGGAGGCCTTCGAGCGCGCGGGCGTC
GACCCGGCCGCCGTCGCCGGCAGCCGCACCGGCGTCTTCGTCGGCACCACCTACACCGGC
TACGGCAGCGACCGGGAGGGCGCCGAGGAGAACGTCGAGGGCCACCTCATGACCGGCATC
GCGACCGCCGTCGCCTCCGGCCGGCTCTCCTACACCTACGGATTCGAGGGCCCCGCCGTC
TCCCTCGACACCATGTGCTCCTCCTCCCTGGTCGCCCTGCACCTGGCCGTCCAGGCGCTG
CGGCAGAAGGAGTGCACCCTCGCGCTCGCGGGCGGCTCGCAGATCATGTCCACGCCGGAC
GTGTACGTGGAGTTCAGCCGCCAGCGCGGCCTCTCCCCGGACGGCCGCTGCAAGCCCTTC
GCCGCCGCCGCCGACGGCACCGGCTGGTCCGAGGGGGTCGGCGTGCTGCTCCTGGAGCGG
CTCTCCGACGCCCGGCGCAACGGCCACCGGGTGCTCGCCGTGGTCCGCGGCTCCGCGATC
AACCAGGACGGCGCCTCCAACGGGCTCACCGCCCCCAACGGGCCCTCCCAGCAGCGCGTC
ATCGAGCAGGCCCTCGCCAACGCCCGGCTCTCCCCGCACCAGGTCGACCTGATCGAGGCG
CACGGCACCGGCACCACCCTCGGCGACCCCATCGAGGCGCAGGCCCTGCTCGCCACCTAC
GGCGGCAGCCGCCCGGAGGGGCGCCCGCTCTGGCTCGGCTCGGTGAAGTCGAACATCGGC
CACGCGGCCGCCGCGGCGGGTGTCGCGGGTGTCATCAAGGCGGTCATGGCGGTACGCGAG
GGCGTGCTCCCCAAGTCGCTGCACATCGACGCCCCCACCCCGGAGGTCGACTGGTCCTCC
GGCGCCGTCGCCCTGCTCACCGAGGAGCGCGAGTGGTCCGTCCCCGGTGAGGAGCCGCGC
ACCGCCGCGATCTCCTCCTTCGGCGCGAGCGGCACCAACGCCCATGTCCTCGTCCAGTAC
GCCCCCGAGCCCGACCCGGAGCCGGCGGCCGAGGCACCCGCCGCGGTCTTCACCGGCGCC
GCGCTGCCCTGGCTCCTCTCCGGCCGTACCGCCGAGGGACTGCGCGGCCAGGCCCGCTCC
CTGCACACCTACGCCTCCACCACCGCCACCGCACTCCCGGGCGCGGCCCTCGGCCTCGCC
ACCACCCGCGCCGCCCTGGAACGCCGCGGCGCGGTCCTCACCTCCGGCACCCCCGGTACC
CCCGACAAGGACGCCCTGCTCACCGGCCTGGACGCCCTCGCCGAGGGCACCCCGGCGGCC
GGGATCCTCGAAGGCACCACGGTCAGCGGCGCCGACCGCCCCGTCTTCGTCTTCCCGGGT
CAGGGTTCGCAGTGGGCGGGGATGGCGGTCGAACTCCTGGATTCCTCCTCGGTGTTCGCG
GCTCGTCTGGGGGAGTGCGCGGAGGCGCTGGACCCCTTCGTGGACTGGTCCTTGGTGGAT
GTCCTGCGGCAGACCGAGGGTGCTCCCGGTTTCGATCGCGTCGATGTGGTGCAGCCCGCG
CTCTGGGCCGTGATGGTGTCCCTCGCGGAGGTGTGGCGTGCGGCGGGTGTCGCCCCGGCC
GCGGTGATCGGTCACTCGCAGGGTGAGATCGCGGCTGCGGCGGTGTCCGGTGCGCTGTCC
CTGTCGGATGCGGCGAAGGTTTCGGCGTTGCGGGCGCGGGCGTTGCTGGCGCTGGCGGGC
AAGGGCGGGATGGTCTCGGTCGCGGATGCGGCCGATTCCGTACGGGAGCGGATCTCCGCT
TGGGGTGAGCGGCTTGCGCTCGCTTCGGTGAACGGTCCGCAGTCGACGGTGGTTTCGGGT
GATCCCGAGGCGCTGGACGAGTTGATGGCGGGCTGTGAGGCGGAGGGGGTGCGGGCCCGG
CGGATCAATGTGGACTACGCCTCGCACGGCCCCCAGGTGGAGAAGATCCGGACCGAGGTC
CTGGGCGCGCTCTCCGGTATCGAGCCGCGCACCGCCGAGGTGCCCTTCCTGTCCACGGTG
ACCGGCGAGTTTGTGACCGGCACCGAGCTGGACGCCGAGTACTGGTACCGCAACCTGCGC
AACACGGTCCGATTCGAGGACGCCGTACGGCAGTTGCTGGACCGGGGCCACGGGGCCTTC
GTGGAGGCGAGTGCCCATCCGGTGCTGACCGTCGGCGTGCAGGAGACCATCGACGCGGCG
GGCGCGCCCGCCTTCGTCCAGGGCACCCTGCGCCGCGAGGACGGCGACGCGGCCCGCTTC
CTCGCCTCCCTCGCCGAGGCCTGGACCCGTGGTGTGCCCGTCAACTGGGCCATCGTCACG
GACAGTTCGGCGCCCCCGGCCGAGGATCTGCCGACCTACGCCTTCCAGCGCCGCACCTAC
TGGCTGCACGGCGCCCGGACCGGCTCGGTCCAGGCCCCGGTGGACGCCGTGGAGGCCGAG
TTCTGGGACTCCGTGGAGAACGGGGACATCGACTCGCTCTCCGGTTCCCTCGCCCTGGAG
GACAGCGCGCCCCTGGCGGAGCTGCTCCCGGCACTCTCCTCCTGGCGGCGCCGCAGGCGG
GAGCGCGGCGAGGTCGACAGCTGGCGCTACCGGATCGACTGGCAGCCGCTGGCCGAGAGC
GCACCGCCCGCCCTGGAGGGGACCTGGCTGCTCGTCACCGGGGACGGGGTCGAGGCCGAG
ATCCTGAAGACGGGGGAGTCGGCGCTCAGCGCGCACGGCGCCACCGTGCACCCGCTGACC
CTCACGGGGGAGGGCGAACGCGAAGCCCTCGTACGGCAGTTGCTGGGCGCCGAGGTCGAG
CACGGCCCCTTCGCCGGGGTGCTCTCGCTGCTCGCCACCGCGGAGCCGGAGCGGGGGCCC
GCCACCACCCTGGCGCTCGTCCAGGCCCTGGCGGACGCGGAGTGCGAGGCGCCGCTGTGG
GTCGCCACCCGCGGCGCCGTCGGCACCGGACCCGAGGAAGCACCCGCACACCCCGCGCAG
GCGGGGGCCTGGGGCCTCGGCCTGGTCGCCGCCCTGGAGCGGCCCGGCGGCTGGGGCGGC
CTCGTCGACCTGCCCGCCGAGCCGGACGAGAACCTGGCCGGGCGGCTCGCCGCCGCGCTC
GCGGGGCAGGAGGACCAGCTCGCGCTGCGGGCCACCGGCACCTATGTGCGCCGACTGGCG
CGCGCCCCGCTGCCCGGCTCCGAGGTGCGGCCCTGGGAACCCGCCGACACCGTGCTCGTC
ACCGGCGCGACCGCGCCCGTCGGCGCCCGTACCGCCCTGCTGCTCGCGGCCTCCGGCGCC
AAGCGCCTGCTGCTCACCGTGCCCGGCGGGGAGGAGCAACCTGCCGCGCTGGTGGGGGAG
TTGGAGGAAGCCGGGGTCCAGGTCACGCTCGCGGAGTGGGACGGCCGGGACGTCACCGCG
CTGCGGTCCCTCGCGGAGGCGGCCGCCGCGGACGGCGCCCCCGTCCGCGGGGTCTTCCAC
GCCGCCACCCGGGCCGACCTCGCCCCGCTCGACGAGACCACCGCCGCCGACCTCGCCGCC
GCCACCGCGGCGAAGACCGTCCCGGCACGCGCCCTGGACGAGGCCTTCGGCGAGGAGGTC
GAGGCCTTCGTGCTGTTCTCCTCGGTCACCTCCTACTGGGGCGGCGGGGAGCACGCCGCC
TTCGCCGCCGCGAGCGCCGAGCTGGACGCGCTCGCCGCCCGCCGCCGGTCCCGCGGACTC
GCCGCCACCTCGGTGGCCTGGGGCGTCTGGGACCTCTTCGACGCCGAGCAGAACCCGGCG
GAGGCCGCCGAACTCCAGGCCCGCTCCGCGGACCGCGGACTGCCGCTGCTCGACCCCGAG
ACCGCCTGGCAGGCGCTGCGGCTCAGCCTCGGCAGGCAGGAGACCGCGATCGCCGTCGCG
GACGTGGACTGGGAGCGGTTCTGGCCGCTGTTCACCTCCGCGCGACCCGCCCCGCTGCTC
TCGGACCTGCCCGAGGTGCGCGGCCTCGGCCGGGGCCTGGCCGAGGACACCGGCACCGGG
GCCGATCCCGGCGCCGCCGAGGCCCTGCGGAGCAAGCTCGCCGGGCTCTCCCCGGCCGAA
CAGGACCGGGCCCTCACCGACCTGGTCTGCGCCCACGCCGCCGCCGTCCTCGGGCACTCC
TCCGCGGGCGCCGTCGACGCCGAACGCGCCTTCAAGGACCTCGGCTTCGACTCGCTCACC
GCGGTGGGCCTGCGCAACGGCCTGGGCGCCGCCACCGGCCTCAGCCTGCCCGCCACCCTC
GTCTTCGACTACCCGACGCCCGCCGCGATGGCCGGTTACGTACGCGATCACCTGCTGGCC
GGGGCACGCCAGGAGGCGACCGCCGCCGGGGTGCAGTCGGGCCTGGACCAGCTGGAGGCG
GACCTGCTGTCCGTCGCCCTGGACAAGGACGAACGCAAGAACCTCACCCGGCGCCTCGAA
GGACTTCTGTCCCGCTTCAAGGACGCCCAGGCCGCCGCCGACGAGGAGTCGGTGAGCGGA
AAGCTCGATTCGGCTTCCGACGAAGAGATCTTCGCCTTCATCCGTGAGGAATTCGGAAGG
CCCGAGTAG
[7] KS34..410
[7] AT575..893
[7] methylmalonyl-CoA766..770
[7] kr1196..1373
[7] ACP1485..1555
[7] KS100..1230
[7] AT1723..2679
[7] methylmalonyl-CoA2296..2310
[7] kr3586..4119
[7] ACP4453..4665

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[765-882] 2.8e-68 G3DSA:3.40.366.10
G3DSA:3.40.366.10   no description
IPR006162 Phosphopantetheine attachment site (PTM)
[1513-1528] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1485-1555] 19.18 PS50075
PS50075   ACP_DOMAIN
[1484-1558] 1.6e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
[1488-1554] 2.7e-10 PF00550
PF00550   PP-binding
[1477-1569] 2.3e-23 SSF47336
SSF47336   ACP-like
IPR013968 Polyketide synthase, KR (Domain)
[1197-1371] 1.1e-40 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-284] 3.4e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[292-410] 5.3e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[575-893] 1.1e-108 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-27] 3.6e-12 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[573-865] 6.1e-64 SSF52151
SSF52151   FabD/lysophospholipase-like
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[700-765] 6.4e-18 SSF55048
SSF55048   Probable ACP-binding domain of malonyl-CoA ACP transacylase
IPR016038 Thiolase-like, subgroup (Domain)
[36-296] 8e-88 G3DSA:3.40.47.10 [297-463] 7e-61 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
[26-463] 8.1e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1196-1371] 6.8e-18 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[577-874] 3.3e-111 SM00827
SM00827   Acyl transferase domain in polyketide syntha
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1486-1558] 4e-33 SM00823
SM00823   Phosphopantetheine attachment site
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-463] 1.3e-215 SM00825
SM00825   Beta-ketoacyl synthase
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1196-1373] 7.6e-17 SM00822
SM00822   no description
SignalP No significant hit
TMHMM No significant hit
Salino3_00150 Salino3_00150   Salino3_00170 Salino3_00170
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