Salino3_00250 : CDS information

close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction94,936 / 94,655 / - [in whole cluster]
94,936 / 94,655 / - [in contig]
Locationcomplement(94655..94936) [in whole cluster]
complement(94655..94936) [in contig]
TypeCDS
Length282 bp (93 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.5 electron carrier
Productputative ferredoxin
Product (GenBank)putative ferridoxin protein
Gene
Gene (GenBank)slnE
EC number
Keyword
Note
Note (GenBank)
  • SlnE
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

SlnE (93aa) : Ferredoxin

P450 monooxygenase SlnFとferredoxin SlnEはC-20でのhydroxylationを成し遂げるようだ。
しかし、置換によるslnF mutantはsalinomycin産生性を完全に失ったので、SlnFによるhydroxylationは成熟したpolyketide鎖がACPから放出される前に起こるかもしれない。
Related Reference
ACC
H1ZZU5
NITE
Salino_01030
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
配列は[H6D581]と全く同じ。株違い。

monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

salF (93aa) : ferredoxin

詳細コメントなし。
Family/Domain
FD
IPR001080
comment
[IPR001080] 3Fe-4S ferredoxin (Family)
function: electron carrier activity, iron ion binding

close this sectionSequence

selected fasta
>putative ferredoxin [putative ferridoxin protein]
MSAAGTPTAREETGGGSRDGDWEVSVDRLSCTGTGMCVHAAPAFFGLVGGRSQALGSPVE
PDETVLDAAENCPLEAIRVTERATGRVLAPEEF
selected fasta
>putative ferredoxin [putative ferridoxin protein]
GTGAGCGCCGCGGGGACGCCGACCGCGCGGGAGGAGACCGGCGGCGGGTCGCGGGACGGC
GACTGGGAGGTCAGCGTCGACCGGCTGTCCTGCACCGGCACCGGCATGTGCGTCCACGCG
GCGCCCGCCTTCTTCGGCCTGGTGGGCGGCCGTTCGCAGGCGCTCGGCTCCCCCGTGGAG
CCCGACGAGACGGTGCTGGACGCCGCCGAGAACTGCCCCCTGGAGGCCATCCGCGTCACC
GAACGGGCCACCGGACGGGTGCTGGCGCCCGAGGAGTTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001080 3Fe-4S ferredoxin (Family)
 [26-37]  6.1e-05 PR00352 [37-47]  6.1e-05 PR00352 [65-77]  6.1e-05 PR00352
PR00352   3FE4SFRDOXIN
SignalP No significant hit
TMHMM No significant hit
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