Vanco_00030 : CDS information

Location

Organism	Amycolatopsis orientalis
Strain	ATCC 19795 (=NBRC 12806)
Entry name	Vancomycin
Contig	HE589771
Start / Stop / Direction	2,245 / 2,853 / + [in whole cluster] 2,245 / 2,853 / + [in contig]
Location	2245..2853 [in whole cluster] 2245..2853 [in contig]
Type	CDS
Length	609 bp (202 aa)

Annotation

Category	4.4 resistance
Product	putative D-alanyl-D-alanine dipeptidase
Product (GenBank)	dipeptidase
Gene
Gene (GenBank)	vanX
EC number	3.4.13.22
Keyword
Note
Note (GenBank)
Reference	ACC G4V4Q5 comment A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、報告論文なし。
Related Reference	ACC Q6ZZK1 NITE Teicp2_00130 PmId [17220405] Resistance to glycopeptide antibiotics in the teicoplanin producer is mediated by van gene homologue expression directing the synthesis of a modified cell wall peptidoglycan. (Antimicrob Agents Chemother. , 2007) comment BLAST id82% Actinoplanes teichomyceticus_tcp4 Dipeptidase(VanX protein) teicoplaninの産生株A. teichomyceticus ATCC 31121における自己耐性の包括的研究。 vanHat, vanAat, vanXat genesはユニークなpolycistronic mRNAで同時転写されることが示された。 vancomycinまたはteicoplanineを添加してもvanXat転写物量にはほとんど影響がなかったことから、恒常的な転写が示唆された。(data not shown)
	ACC Q9ZI81 NITE A4793_00330 PmId [9751644] Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. (Chem Biol. , 1998) comment BLAST id80% Streptomyces toyocaensis NRRL 15009_vanXst D-ala-D-ala dipeptidase ・Streptomyces toyocaensis (StoVanX) ・Escherichia coli (EcoVanX) ・Synechocystis sp. strain PCC6803 (SynVanX) におけるvanX homologsを明らかにし、D,D-dipeptidase触媒活性を検証。 StoVanXは高効率なD-Ala-D-Ala dipeptidaseであり、D-Ala-D-lactate depsipeptide活性を欠く。
	ACC R4SV78 PmId [24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014) comment BLAST id86% Amycolatopsis orientalis HCCB10007_vanX(AORI_1473) vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。 AORI_1473(vanX): D-alanyl-D-alanine dipeptidase このORFに関しては配列解析のみ。

Sequence

>putative D-alanyl-D-alanine dipeptidase [dipeptidase]
MKDDFVYVDEVVPGIRWDAKYATWDNFTGKPVDGYLVNRIVGTKALCAALEKARDKAESL
GFGLLLWDGYRPQRAVNRFMSWAEEPEDGRKKSRHYPNIDRPQMFEKGYVATKSGHSRGS
TVDLTIYHLATGELAPMGGDHDLMDVVSHHGAEGVTPEEAKNRLHLRAIMEDCGFNSYEY
EWWHYNLKDEPYPDTYFDFPIE

>putative D-alanyl-D-alanine dipeptidase [dipeptidase]
ATGAAGGATGATTTCGTCTACGTAGACGAGGTCGTGCCCGGAATCCGCTGGGACGCCAAG
TACGCCACCTGGGACAACTTCACCGGCAAGCCGGTGGACGGGTACCTCGTGAATCGGATC
GTCGGCACCAAGGCGCTGTGCGCGGCCCTGGAAAAGGCGAGGGACAAGGCCGAGTCTCTC
GGCTTCGGCCTGCTCCTCTGGGACGGTTACCGTCCTCAGCGTGCCGTGAACCGCTTCATG
AGCTGGGCGGAAGAGCCCGAGGACGGCCGGAAGAAGTCCCGGCACTACCCGAACATCGAC
CGGCCCCAGATGTTCGAAAAGGGCTACGTGGCGACGAAGTCGGGGCACAGCCGGGGAAGC
ACGGTGGACCTGACGATCTACCACCTGGCCACCGGCGAACTCGCCCCGATGGGCGGCGAC
CACGACCTGATGGACGTCGTCTCGCATCATGGCGCCGAGGGGGTCACGCCGGAAGAGGCG
AAGAACCGCCTGCACCTCCGCGCCATCATGGAGGACTGCGGCTTCAACTCGTACGAGTAC
GAGTGGTGGCACTACAACCTGAAGGACGAGCCTTACCCGGACACCTACTTCGACTTCCCG
ATCGAATAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000755 Peptidase M15D, VanX D-ala-D-ala dipeptidase (Family) PIRSF026671 AA_dipeptidase PF01427 Peptidase_M15 IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain (Domain) SSF55166 Hedgehog_sig_N G3DSA:3.30.1380.10 Hedgehog/DD-pept_Zn-bd
SignalP	No significant hit
TMHMM	No significant hit

Vanco_00020 Vanco_00040