Vanco_00070 Vanco_00070   Vanco_00090 Vanco_00090

Vanco_00080 : CDS information

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Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction8,917 / 18,357 / + [in whole cluster]
8,917 / 18,357 / + [in contig]
Location8917..18357 [in whole cluster]
8917..18357 [in contig]
TypeCDS
Length9,441 bp (3,146 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)non-ribosomal peptide synthetase A
Gene
Gene (GenBank)vpsA
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
comment
A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、cluster報告論文なし。個々のタンパクなどでの続報もなし。
Related Reference
ACC
R4SV84
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)
comment
BLAST id83%
Amycolatopsis orientalis HCCB10007_(AORI_1478)

vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。

AORI_1478(vcmA): Non-ribosomal peptide synthetase

vcmAに関しては配列解析のみ。
Figure 5でのVcmAのドメイン構造は、
M1: A-T (Leu1)
M2: C-A-T-E (Bht2)
M3: C-A-T (Asn3)
ACC
O52819
NITE
Chlor_00030
PmId
[10716695] Heterologous expression in Escherichia coli of the first module of the nonribosomal peptide synthetase for chloroeremomycin, a vancomycin-type glycopeptide antibiotic. (Proc Natl Acad Sci U S A. , 2000)
comment
BLAST id82%
Amycolatopsis orientalis (Nocardia orientalis)
PCZA363.3
chloroeremomycin clusterにおけるORF3/CepAに相当するentry.

chloroeremomycinのN末AAはD-methylleucineであるが、対応するmodule 1やその近くにepimerase domainや dual condensation/epimerization domainは見られない。

・CepA1-575 (A1-PCP1)
・CepA1-1596 (A1-PCP1-C-A2-PCP2)
・CepA1-3158 (full-length = A1-PCP1-C-A2-PCP2-E-C-A3-PCP3)
をE. coliで発現、精製して実験に使用している。

CepA1-575によるATP-PPi exchange assaysで、A1 domainの特異性を調査。特異的にL-Leu と D-Leuを活性化し、L-Leuのほうが6倍好まれる。L- or D-N-methylleucineの活性化は見られない。

balhimycinでの実験結果をふまえ、Leuのメチル化はheptapeptide合成と酸化的環化の後に起こることが示された。
LeuのL→Dへのepimerizationのタイミングについても検討しているが、実験確認はしていない。transで働くE domainか、clusterの外側にあるracemaseが作用するのかもしれない。

CepA1-575をCoA and Sfpで処理するとholo型となり、これにL-Leu and ATPを加えるとL-Leuの取り込みが観察された。

CepA1-575で観察された活性は相対的に低く、CepA1-1596 と CepA1-3158 を用いた実験はうまくいかなかったことから、chloroeremomycin NRPS genesの発現には他の手法や改善が望まれている。

close this sectionPKS/NRPS Module

A1 leucine
2 beta-hydroxytyrosine(Bht)
3 asparagine
A36..427
PCP503..569
C600..892
A1058..1448
PCP1523..1589
E1623..2075
C2088..2379
A2560..2962
PCP3044..3110

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [non-ribosomal peptide synthetase A]
MNSAARTPATVLELFASRVGRTPGAVAVVDGDRVLTYRELDEAAGLLAGRLAGRGIRRGD
RVAVMMGRSADLLVALLAVWKAGATYVPVDAAYPAPRVEFMVRDSAAALMVCSVASRDAV
PEGVDSLVVDAVTDEDGRAPTATVRPEDLAFVMYTSGSTGRPKGVAITQGSVADLVRDPG
WAMGPDDAVLMHSPHAFDASLFEVWTPLASGARVVIAEPGSVDVRRLRAAAAAGVTRVYL
TAGSFRAVAEEAPESFAAFREVLTGGDVVPAHAVQRVRQACPEARFRNMYGPTEATMCAT
WYPLEPGEVLGAVVPIGRPLAGRRVQVLDESLRPVEPGVVGDLYLSGCLAEGYFGRPGLT
AERFVADPSAPGQRMYWTGDLAQWTADGELLFAGRADDQVKVRGFRIEPGEIEAALIAQP
EVHDAVVAAIEGRLIGYVVADEDVDPVVVRERLRAELPEYLVPVVVLTLDALPLTRNGKV
DRAALPAPEFATGAAGREPATEEERVLCGLFADVLRLDRAGVDDGFFDLGGDSIFAMRLA
ARAAKAGLLVTPVQIFEEKTPARLAAVARPVPADEPLDGPLLDLTEAEEAELAIAAPGAE
EIWPLAPLQEGLLFESILDDEGSDIYQVQVILELNGPLDEARLRAAWDTVVHRHPELRLT
FHRLESGKTVQAVHGDVAVPWRAVDLAGADDVDAAVGALAGQEQGKRFDLAKAPLVRLVL
IRIGEDRHRLLFVIHHILVDGWSVAVILNEVADAYTAGGRLPEQRGGASFKDYLAWLDRQ
DKDAARAAWRDELAGLDEPALIATTSVETDYDYRATHLTPALHTRLLEFAREHGLTPSTV
VHAAWALVLARLTRRTDVVFGTMVATRPAELEGIESMPGLLMSAVPVRVPLDGGQPVVDL
LTDLHTRQTALKPHQHLGLPEIQKAAGPGAGFDTLLVIENYPREYAAQFTHLRTIEGTHY
PLTLGITPGERFKIQLGYWQGQVPDAVAESLLEWFVGAIGALVADPARPVGRIGVGTAET
REWAAPLVAGEPLPSLVGRIAARLPDNVALVDSEGELSYAELWERSLKLAAVLRDNGVGS
ESRVGLVVGRSSWWTVGMLGVLLAGGTFVPVDPAYPDERKEWIFRSADPMLVVCEGTTRG
AVPVEFADRLLVVDDVDLSATPEAELPRVDPRSAAYVIYTSGSTGTPKGVVVTHTGLGNL
ALAHIDRFRVTPSSRVLQFAALGFDTIVSEVMMALLSGATLVVPPEQELPPRMAFSETLE
RWGITQVKAPPSVLGTVDALPPTVETVVAAGELCPPGLVDRLSADRRMINAYGPTETTIC
ATMSLPLTPGQHPIPFGGPVPGVRGYLLDSFLRPLPPGVTGELYLSGIGVARGYLGRPGL
TAERFVADPFAPGERMYRTGDLAYWTDQGELVSAGRADDQVKIRGFRVEPREIEFALSAY
PRITQATVTVREDRLLAYVTPGDIDTQAVRKHLASRMPQYMVPAAVVALDTLPLTAHGKI
DRRALPDPEFTTGAPAREPATETERVLCELFAGVLGLEQVGVDDSFFDLGGDSILSMQLA
ARARRSGLTFSAADVFDGKTPERIARLAEESAPPEPGRPAKSDGVGEVPWTPVTRMLGDG
VTGSAFAQWMVVGAPADLTEKTVAAGFAAVVDAHDMLRARVVDDESGRRLLVGERGSVTG
SVTRVGLDGRPVDEVVEEAARDAVSKLDPSAGVLARAVWVDAGPDRIGRLVVVVHHLSVD
GVSWRVLLSDLQSACEAVAAGREPVLEPVEVSFKQWAGLLGEWAVSAERVGELAAWKAIL
GQEQPAATRELTTSQAAKGEVRSRSWVVPKTETSALVGRAPVVFHCGVHEILLAGLAGAV
TRWRGGDTVLVDVESHGRHPADGMDLSRTVGWFTSAHPVRLEVAGTELADVLAGGPAAGR
LLKTVKEQSRAVPGDGLGYGLLRYLNDTTAPVLAELPSARIGFNYMGRFATGEDSGVRAW
QPVGDIGSSLEPGMGLPHAIEVNAIVQDLPDGPELTLMLEWQEDLLGEAEIDQLGQGMLA
VLAGLARQAEDPAAGGHTASDFALVALGQDEIEALEAEFADAGGVADLLPLSPLQHGLAF
HAGFSGDGVDVYTAQAVLELTGPLDMPLLRKSVRALLDRHANLRAGFRHAADGSAYQVIP
GALAVPVTLVDVTGSADPEAEAAAVAAAERARPFDLAKPPLLRLTVVALGENDHRLVLTN
HHILLDGWSTPLLLDELLTLYRDGAVPSALAPVNPYREYLAWVRETDRDAAITAWRDALA
GLPEPTLVAADRAVPVQVPGQMWAELDEAFAGALAARARECGVTVSTMLQAVWGMVLAAI
TGRDDVVFGAVVSGRPAELPGIETMVGLFINTVPVRVRMEPRHTFAELVKEVQDEQIALL
AHHHVGLTDIQQTAGLGRLFDTLVVYENYPRPAETADAEPDRLRVLGMTAADATHYPLAL
AVVPGESLRLRLEHQPAMFTTEQAAAVLDRFTRVLEAVVADPGTPLAAVPILSESEQREL
RARNDTAVPVPDRTLPELFAAQVAATPDATAVTFEDRSLSYAELDARADRLARRLIERGV
GPEGLVAVMMPRSLDLVVTVLAITKTGGAWLPIDPVYPADRVTYILDDARPALVITSAAL
ADSPAGEVLAARPATVVLDDLATAGGEHTPVTDADRIRPLDPRHPAYLIYTSGSTGRPKA
VVVTHRNLANYLFHCGKKYPGLRGRSLLHSSIAFDLTVTAMLTPLTVGGTVQVGALEDLI
GTVESAPALFLKATPSHLQTLETGSREIAVPGDLLLGGEQLSVETVADWRRTYPNTVVVN
EYGPTEATVGCVEYRLEPGQAAPSGDVVPIGTPMTNMRAFVLDSWLRPVPPGAVGELYVS
GAGVARGYLGRAGLTATRFVADPFGAGELMYRTGDLVRWNPDGELVFAGRVDDQVKVRGY
RIEPGEIEAALEAHESVGQAVVLARDSASGKRLIGYVTPAGDTGVDVAAVRAAVAARLPE
YMVPAAVVVLDALPLTANGKVDRDALPDPDFNERVSGREPVTEAERLLCALFAEVLGLER
AGADDSFFELGGDSILSMRLAARAHREGMAFGAREVFELRTPAGIAAIVERAPDVPAAAD
YAGSDVALLELNQDEIDEFEAEFDAS
selected fasta
>non-ribosomal peptide synthetase [non-ribosomal peptide synthetase A]
ATGAATTCCGCAGCGCGGACCCCCGCGACGGTGCTGGAGCTGTTCGCCTCGCGGGTGGGG
CGGACGCCCGGCGCGGTGGCCGTGGTCGACGGCGACCGGGTGCTGACCTACCGGGAACTC
GACGAGGCCGCGGGCCTGCTGGCCGGACGGCTGGCGGGCCGGGGCATCCGCCGTGGCGAC
CGGGTCGCGGTGATGATGGGCCGCTCGGCGGATCTGCTGGTGGCGCTGCTCGCCGTGTGG
AAGGCGGGGGCGACGTACGTACCGGTGGACGCCGCCTATCCGGCGCCGCGGGTGGAATTC
ATGGTGCGCGATTCGGCGGCCGCGCTGATGGTGTGCTCGGTCGCGTCGCGTGACGCCGTA
CCGGAGGGCGTCGATTCGCTCGTGGTGGACGCCGTCACGGACGAGGACGGGCGTGCGCCG
ACGGCCACGGTGCGCCCGGAGGACCTGGCGTTCGTGATGTACACGTCCGGCTCCACGGGC
AGGCCCAAGGGGGTGGCGATCACCCAGGGCAGTGTCGCGGACTTGGTGCGGGACCCCGGT
TGGGCGATGGGGCCGGACGACGCCGTGCTGATGCATTCACCGCACGCCTTCGACGCGTCC
CTGTTCGAGGTCTGGACGCCGCTGGCCTCGGGCGCGCGGGTGGTGATCGCCGAACCGGGT
TCGGTGGACGTGCGGCGCCTGCGCGCCGCGGCCGCGGCCGGGGTGACGCGGGTGTACCTG
ACCGCGGGGAGTTTCCGCGCGGTGGCGGAGGAGGCGCCGGAGTCGTTCGCGGCGTTCCGT
GAGGTGCTGACCGGCGGTGACGTGGTGCCCGCGCACGCGGTGCAGCGGGTGCGGCAGGCC
TGTCCCGAGGCGCGGTTCCGGAACATGTACGGGCCGACGGAAGCGACGATGTGCGCGACG
TGGTACCCGCTGGAACCGGGCGAGGTGCTGGGTGCGGTGGTGCCGATCGGACGTCCGCTG
GCCGGCCGCCGGGTCCAGGTGCTCGACGAGTCGCTGCGCCCGGTGGAGCCGGGCGTGGTC
GGCGACCTGTACCTCTCCGGATGTCTGGCGGAGGGCTACTTCGGCCGGCCGGGGCTGACG
GCGGAGCGGTTCGTCGCGGATCCCTCCGCGCCGGGACAGCGGATGTACTGGACGGGTGAC
CTCGCCCAGTGGACGGCGGACGGTGAGCTGCTGTTCGCGGGCCGGGCCGACGACCAGGTG
AAGGTCCGCGGGTTCCGGATCGAGCCCGGCGAGATCGAGGCCGCGCTGATCGCCCAGCCG
GAGGTCCACGACGCCGTGGTCGCGGCGATCGAGGGACGGCTGATCGGCTACGTGGTGGCC
GATGAGGACGTGGATCCCGTCGTCGTCCGCGAGCGCCTCCGCGCGGAATTGCCGGAATAC
CTGGTTCCCGTCGTCGTCCTCACGCTGGACGCGTTGCCCTTGACCCGTAACGGAAAGGTG
GACAGGGCGGCGCTGCCCGCGCCCGAGTTCGCGACGGGCGCCGCGGGCCGTGAACCCGCC
ACCGAGGAGGAACGCGTCCTTTGTGGACTGTTCGCCGACGTGCTCCGGCTCGACCGGGCC
GGGGTCGACGACGGCTTCTTCGACCTGGGCGGGGATTCGATCTTCGCGATGCGCCTGGCG
GCACGCGCGGCCAAGGCGGGCCTGCTGGTGACACCCGTCCAGATCTTCGAGGAGAAGACC
CCCGCGCGGCTGGCGGCGGTGGCGCGTCCGGTACCGGCCGACGAGCCCCTCGACGGCCCC
CTGCTCGATTTGACCGAAGCGGAGGAGGCGGAGCTGGCGATCGCCGCTCCGGGTGCCGAG
GAGATCTGGCCGCTGGCGCCGTTGCAGGAGGGGTTGCTCTTCGAGTCGATCCTGGACGAC
GAGGGCTCCGACATCTACCAGGTGCAGGTGATCCTGGAGCTGAACGGGCCGCTGGACGAA
GCCCGGCTGCGGGCCGCGTGGGACACGGTCGTCCACCGGCACCCCGAGCTTCGGCTCACC
TTCCACCGGCTCGAGTCGGGCAAGACGGTGCAGGCCGTCCACGGGGACGTCGCCGTGCCG
TGGCGGGCGGTGGATCTGGCGGGTGCCGACGACGTCGACGCGGCCGTCGGGGCCTTGGCC
GGACAGGAACAGGGAAAGCGGTTCGATCTCGCCAAGGCGCCGCTGGTCCGGCTGGTGCTG
ATCCGGATCGGCGAGGACCGGCACCGGCTGCTGTTCGTCATCCACCACATCCTGGTCGAC
GGCTGGTCGGTGGCGGTGATCCTCAACGAGGTCGCCGACGCGTACACGGCGGGCGGACGG
CTGCCGGAACAGCGCGGCGGCGCCTCCTTCAAGGACTACCTGGCGTGGCTGGACCGGCAG
GACAAGGACGCGGCGAGGGCGGCCTGGCGCGACGAGCTGGCCGGTCTCGACGAGCCCGCG
CTGATCGCGACGACGTCCGTCGAGACGGACTACGACTACCGCGCCACCCACCTGACCCCG
GCGCTGCACACCAGGCTGCTGGAGTTCGCCCGTGAGCACGGGCTGACGCCGAGCACGGTG
GTGCACGCCGCCTGGGCGCTGGTGCTGGCGCGTCTCACGCGGCGGACCGACGTGGTGTTC
GGCACCATGGTCGCGACCCGCCCCGCGGAACTCGAGGGTATCGAGTCGATGCCGGGCCTG
CTGATGAGCGCGGTGCCGGTGCGGGTGCCCCTCGACGGCGGCCAACCGGTCGTGGACCTG
CTGACCGACCTGCACACCAGGCAGACGGCCCTCAAACCGCATCAGCATCTGGGCCTGCCG
GAGATCCAGAAGGCGGCCGGACCCGGCGCGGGCTTCGACACGCTGCTGGTGATCGAGAAC
TACCCGCGGGAGTACGCCGCTCAGTTCACGCATCTGCGCACCATCGAAGGCACGCACTAT
CCGCTGACCCTCGGCATCACGCCCGGGGAGCGGTTCAAGATCCAGCTCGGCTACTGGCAG
GGACAGGTTCCCGACGCCGTCGCCGAATCGCTGCTGGAGTGGTTCGTCGGCGCGATCGGC
GCGCTGGTCGCCGATCCCGCCCGCCCGGTGGGGCGGATCGGGGTGGGCACGGCCGAGACG
CGCGAGTGGGCCGCGCCGTTGGTGGCGGGCGAGCCGCTGCCGTCGCTGGTCGGACGGATC
GCGGCGCGGCTGCCGGACAACGTGGCGCTGGTGGACAGCGAGGGTGAGCTGTCCTACGCG
GAGTTGTGGGAGCGGTCGCTGAAGCTCGCGGCCGTGTTGCGGGACAACGGTGTCGGGTCC
GAAAGCCGGGTCGGCCTGGTGGTGGGGCGCTCGTCCTGGTGGACGGTCGGCATGCTGGGC
GTGTTGCTGGCGGGCGGCACTTTCGTGCCGGTGGACCCGGCCTATCCGGACGAGCGCAAG
GAATGGATCTTCCGGAGCGCGGATCCGATGCTGGTGGTGTGCGAGGGGACGACGCGGGGA
GCGGTGCCCGTCGAGTTCGCGGACCGGCTGCTGGTGGTCGACGACGTCGACCTGTCGGCG
ACTCCGGAAGCGGAGCTGCCGCGAGTGGATCCGCGCAGCGCCGCGTACGTGATCTACACG
TCGGGGTCGACGGGAACTCCGAAGGGAGTCGTCGTCACCCATACCGGCCTGGGAAACCTG
GCGCTGGCGCATATCGACCGGTTCCGGGTGACGCCGTCGTCGCGGGTGCTGCAGTTCGCC
GCGCTCGGCTTCGACACCATCGTGTCCGAGGTGATGATGGCGTTGCTGTCGGGCGCGACG
CTGGTGGTGCCGCCGGAGCAGGAACTGCCGCCCCGGATGGCGTTCTCCGAAACGCTGGAA
CGGTGGGGCATCACGCAGGTGAAGGCGCCGCCGTCGGTGCTGGGCACGGTCGACGCGCTG
CCGCCGACCGTGGAAACGGTGGTGGCGGCGGGCGAACTCTGCCCGCCGGGTCTGGTGGAC
CGGCTGTCCGCGGACCGGCGGATGATCAACGCCTACGGGCCGACCGAGACCACGATCTGC
GCGACGATGAGCCTGCCGCTGACGCCGGGTCAGCATCCGATCCCGTTCGGTGGCCCGGTT
CCCGGGGTGCGCGGATATCTGCTGGACTCGTTCCTGCGTCCGCTGCCTCCCGGGGTGACC
GGTGAGCTGTACCTTTCCGGGATCGGGGTGGCCCGCGGCTATCTGGGCCGTCCGGGGCTG
ACGGCGGAGCGGTTCGTCGCCGATCCGTTCGCCCCCGGTGAGCGGATGTACCGGACCGGG
GACCTGGCCTACTGGACCGACCAGGGTGAGCTGGTGTCCGCCGGGCGCGCCGACGACCAG
GTCAAGATCCGCGGGTTCCGGGTCGAACCCCGCGAGATCGAGTTCGCCTTGTCCGCCTAC
CCCCGGATCACGCAGGCCACGGTGACCGTCCGCGAAGACCGCCTGCTCGCCTACGTGACA
CCGGGCGACATCGACACGCAGGCGGTGCGGAAACATCTCGCGTCCCGGATGCCCCAGTAC
ATGGTGCCCGCGGCGGTGGTGGCGCTGGACACCCTGCCGCTGACGGCGCACGGGAAGATC
GACCGGCGCGCGCTACCCGATCCCGAGTTCACCACCGGGGCGCCCGCCAGGGAACCGGCC
ACCGAGACCGAGCGGGTGCTGTGCGAGTTGTTCGCCGGTGTCCTCGGTCTGGAGCAGGTC
GGGGTGGACGACAGTTTCTTCGATCTGGGCGGGGACTCCATCCTTTCGATGCAGCTGGCG
GCGCGGGCACGGCGTTCGGGGCTGACGTTCTCCGCCGCCGACGTCTTCGACGGGAAGACA
CCGGAGCGGATCGCGCGGCTGGCGGAGGAATCGGCGCCGCCGGAGCCGGGGCGTCCGGCG
AAATCCGACGGTGTGGGCGAGGTCCCGTGGACGCCGGTGACGCGGATGCTGGGCGACGGC
GTCACGGGATCGGCGTTCGCGCAGTGGATGGTGGTGGGCGCGCCCGCGGATCTGACGGAG
AAGACGGTGGCGGCCGGGTTCGCGGCCGTGGTGGACGCGCACGACATGCTGCGGGCACGG
GTCGTCGACGACGAGAGCGGCCGTCGCCTGCTGGTCGGCGAGCGTGGTTCGGTCACCGGG
TCGGTCACCCGCGTGGGACTCGACGGCCGTCCGGTGGACGAGGTCGTCGAGGAGGCGGCG
CGCGACGCCGTGAGCAAGCTGGATCCGTCGGCTGGCGTGCTGGCGCGGGCGGTGTGGGTG
GACGCCGGACCGGACCGGATCGGGCGGCTCGTGGTGGTGGTGCACCACCTGTCGGTCGAC
GGTGTGTCGTGGCGGGTGCTGCTTTCGGATCTGCAAAGCGCTTGCGAGGCGGTGGCCGCG
GGCCGGGAGCCGGTGCTGGAGCCGGTGGAGGTGTCCTTCAAGCAGTGGGCGGGCCTGCTC
GGTGAGTGGGCGGTCTCCGCGGAGCGCGTCGGGGAATTGGCGGCGTGGAAGGCGATCCTC
GGACAGGAGCAGCCGGCCGCCACCCGTGAGCTGACCACGAGTCAGGCCGCGAAGGGCGAA
GTGCGCTCGCGGTCGTGGGTGGTGCCGAAGACGGAGACGTCGGCGTTGGTCGGGCGGGCA
CCGGTCGTGTTCCACTGCGGCGTCCACGAGATCCTGCTCGCCGGGCTGGCCGGAGCGGTG
ACGCGGTGGCGTGGCGGGGACACGGTCCTGGTGGACGTGGAAAGCCACGGCCGCCATCCG
GCCGACGGGATGGATCTGTCCCGGACGGTGGGCTGGTTCACCAGCGCGCATCCGGTCCGG
CTGGAAGTGGCGGGGACCGAGCTGGCCGACGTGCTCGCCGGTGGTCCGGCGGCCGGGCGT
CTGCTGAAGACGGTCAAGGAGCAGTCGCGGGCCGTGCCCGGCGACGGGCTCGGCTACGGA
CTGCTGCGCTACCTCAATGACACGACCGCGCCGGTACTGGCGGAACTGCCGTCGGCGCGG
ATCGGGTTCAACTACATGGGCAGGTTCGCCACCGGTGAGGACAGCGGGGTGCGGGCGTGG
CAGCCGGTCGGGGACATCGGCAGCTCGCTCGAACCCGGAATGGGCCTGCCGCACGCGATC
GAGGTCAACGCGATCGTCCAGGACCTGCCGGACGGGCCCGAACTGACGCTCATGCTGGAA
TGGCAGGAGGATTTGCTCGGCGAAGCCGAGATCGACCAACTGGGCCAAGGGATGCTGGCC
GTGCTGGCCGGGCTCGCCCGGCAGGCGGAGGATCCCGCCGCGGGCGGGCACACCGCGTCC
GACTTCGCTCTCGTCGCACTGGGCCAGGACGAGATCGAGGCCCTCGAAGCCGAATTCGCG
GACGCCGGTGGGGTGGCCGACCTGCTGCCGCTGTCACCGTTGCAGCACGGTCTCGCCTTC
CACGCCGGTTTCTCCGGTGACGGCGTCGATGTCTATACCGCGCAAGCGGTGCTGGAACTG
ACCGGTCCGCTGGACATGCCGCTGCTGCGGAAGTCCGTCCGCGCGCTCCTGGACAGGCAC
GCGAATCTGCGCGCCGGCTTCCGGCACGCCGCCGACGGGTCCGCGTACCAGGTGATCCCC
GGTGCCCTGGCGGTGCCGGTGACCCTGGTGGACGTGACCGGGTCGGCGGATCCGGAGGCC
GAGGCGGCGGCGGTGGCCGCGGCCGAGCGGGCCCGGCCGTTCGATCTGGCCAAGCCCCCG
CTGCTGCGGCTGACGGTGGTGGCCTTGGGCGAGAACGACCACCGGCTGGTGCTGACCAAC
CACCACATCCTGCTCGACGGCTGGTCGACGCCGCTGCTGCTGGACGAACTGCTCACGCTG
TACCGCGACGGGGCGGTTCCGTCCGCGCTCGCGCCGGTCAACCCGTACCGGGAGTACCTG
GCCTGGGTGCGCGAAACCGATCGGGACGCGGCGATCACCGCCTGGCGCGACGCCTTGGCG
GGACTGCCGGAGCCGACGCTGGTCGCGGCCGACCGGGCGGTCCCGGTCCAGGTGCCCGGG
CAGATGTGGGCCGAACTGGACGAGGCGTTCGCCGGTGCGCTGGCCGCGCGGGCCCGCGAG
TGCGGGGTCACCGTCAGCACGATGCTGCAGGCGGTGTGGGGCATGGTGCTGGCCGCGATC
ACCGGACGCGACGACGTCGTGTTCGGGGCGGTGGTGTCCGGCCGCCCGGCGGAACTGCCC
GGCATCGAGACCATGGTCGGGCTGTTCATCAACACCGTCCCGGTCCGGGTCCGGATGGAA
CCCCGGCACACCTTCGCCGAACTGGTGAAGGAAGTGCAGGACGAGCAGATCGCCTTGCTG
GCCCATCACCACGTCGGCCTCACCGACATCCAGCAGACCGCCGGACTGGGCAGGCTGTTC
GACACGCTCGTCGTCTACGAGAACTACCCCCGGCCAGCCGAGACCGCCGACGCGGAACCG
GATCGGTTGCGGGTGCTGGGAATGACCGCCGCCGACGCCACGCACTATCCGCTGGCGCTG
GCCGTCGTCCCGGGCGAAAGCCTGCGGCTGCGGCTGGAACACCAGCCCGCGATGTTCACC
ACCGAGCAGGCGGCCGCCGTGCTGGACCGGTTCACGCGGGTGCTCGAAGCCGTGGTCGCC
GATCCGGGGACGCCGCTCGCGGCGGTGCCGATCCTGTCCGAGTCCGAACAGCGAGAGCTG
CGGGCGCGCAACGACACCGCGGTGCCGGTGCCGGACCGGACGCTGCCGGAGTTGTTCGCC
GCGCAGGTCGCCGCCACCCCGGACGCGACCGCGGTGACCTTCGAGGACCGGTCGCTGTCC
TACGCCGAACTCGACGCCCGCGCCGATCGGCTGGCACGCCGGCTCATCGAGCGGGGTGTC
GGTCCCGAAGGCCTGGTCGCGGTGATGATGCCCCGGTCGCTGGACCTGGTCGTCACGGTG
CTGGCGATCACCAAGACCGGAGGAGCCTGGCTGCCGATCGACCCGGTCTACCCGGCCGAT
CGTGTCACCTACATCCTCGACGACGCCCGGCCGGCACTGGTGATCACGAGCGCCGCGCTG
GCGGACTCGCCCGCCGGTGAGGTGCTGGCGGCACGGCCGGCGACGGTGGTGCTCGACGAT
CTCGCGACCGCGGGCGGCGAGCACACGCCGGTCACCGACGCCGATCGGATCCGGCCGCTG
GATCCGCGTCATCCGGCCTACCTCATCTACACCTCGGGCTCGACCGGCCGCCCCAAGGCC
GTCGTCGTCACCCACCGGAACCTGGCGAACTATCTGTTCCACTGCGGGAAGAAGTACCCG
GGGCTGCGGGGACGGTCGCTGCTGCATTCGTCGATCGCCTTCGACCTGACGGTCACCGCG
ATGCTCACCCCGCTCACCGTCGGGGGAACGGTCCAGGTCGGTGCGCTGGAAGACCTGATC
GGCACGGTGGAAAGCGCCCCGGCGCTGTTCCTCAAGGCCACGCCGAGCCATTTGCAGACG
CTGGAGACCGGCTCCCGGGAGATCGCCGTTCCCGGTGATCTCCTGCTCGGCGGCGAACAG
TTGTCCGTCGAGACGGTCGCCGACTGGCGCCGGACGTATCCGAACACCGTGGTGGTCAAC
GAATACGGGCCGACGGAGGCGACGGTCGGGTGCGTCGAATACCGGCTCGAACCCGGGCAG
GCGGCGCCGTCCGGCGACGTGGTGCCGATCGGCACCCCGATGACGAACATGCGGGCGTTC
GTCCTGGATTCGTGGCTGCGGCCGGTGCCGCCGGGCGCGGTGGGCGAGTTGTACGTGTCC
GGTGCGGGCGTGGCACGGGGTTATCTGGGGCGGGCGGGGCTGACCGCGACGCGGTTCGTG
GCCGATCCGTTCGGTGCCGGTGAACTGATGTACCGGACCGGGGACCTGGTGCGGTGGAAC
CCGGACGGGGAACTGGTCTTCGCCGGCCGTGTCGACGACCAGGTGAAGGTGCGGGGATAC
CGCATCGAACCCGGTGAGATCGAGGCGGCACTGGAGGCGCACGAATCGGTGGGGCAGGCC
GTCGTACTGGCCAGGGACAGTGCTTCCGGCAAACGACTGATCGGCTATGTGACTCCCGCC
GGGGACACCGGGGTGGACGTCGCCGCGGTGCGCGCGGCCGTGGCGGCCCGGCTGCCGGAG
TACATGGTGCCCGCGGCGGTGGTGGTGCTCGACGCGCTTCCGTTGACGGCCAACGGGAAA
GTGGACCGGGACGCGCTGCCGGATCCCGACTTCAACGAACGCGTCTCGGGCCGGGAACCC
GTCACGGAGGCCGAACGGCTGCTGTGCGCGCTTTTCGCCGAGGTCCTCGGCCTGGAGCGG
GCAGGCGCGGACGACAGTTTCTTCGAACTGGGCGGGGATTCGATCCTGTCGATGCGACTG
GCGGCGCGGGCGCACCGCGAGGGAATGGCCTTCGGCGCACGCGAGGTGTTCGAGCTGCGG
ACGCCCGCCGGGATCGCGGCGATCGTCGAGCGGGCACCGGATGTGCCCGCCGCGGCGGAC
TATGCCGGGTCCGACGTCGCCCTGCTCGAACTGAACCAGGACGAAATCGACGAATTCGAA
GCCGAGTTCGACGCCTCGTGA
[1] A36..427
[1] leucine198..298
[1] PCP503..569
[2] C600..892
[2] A1058..1448
[2] beta-hydroxytyrosine(Bht)1225..1320
[2] PCP1523..1589
[2] E1623..2075
[3] C2088..2379
[3] A2560..2962
[3] asparagine2735..2830
[3] PCP3044..3110
[1] A106..1281
[1] leucine592..894
[1] PCP1507..1707
[2] C1798..2676
[2] A3172..4344
[2] beta-hydroxytyrosine(Bht)3673..3960
[2] PCP4567..4767
[2] E4867..6225
[3] C6262..7137
[3] A7678..8886
[3] asparagine8203..8490
[3] PCP9130..9330

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BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[36-427] 4.49999999999996e-107 PF00501 [1058-1448] 7.99999999999993e-107 PF00501 [2560-2962] 2.20000000000002e-109 PF00501
PF00501   AMP-binding
IPR001242 Condensation domain (Domain)
[600-892] 5.89999999999994e-63 PF00668 [1623-1903] 1.8e-32 PF00668 [2088-2379] 6.29999999999997e-67 PF00668
PF00668   Condensation
IPR006162 Phosphopantetheine attachment site (PTM)
[1548-1563] PS00012 [3069-3084] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[494-569] 3.30000000000002e-70 G3DSA:1.10.1200.10 [1513-1591] 3.30000000000002e-70 G3DSA:1.10.1200.10 [3036-3112] 3.30000000000002e-70 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[496-570] 1.39999892049878e-15 SSF47336 [1516-1590] 2.39999798157265e-18 SSF47336 [3037-3111] 3.30000526807186e-18 SSF47336
SSF47336   ACP_like
[503-569] PS50075 [1523-1589] PS50075 [3044-3110] PS50075
PS50075   ACP_DOMAIN
[506-566] 2.2e-08 PF00550 [1526-1587] 1.9e-11 PF00550 [3047-3107] 6.80000000000001e-11 PF00550
PF00550   PP-binding
IPR010060 Non-ribosomal peptide synthase (Domain)
[1918-2075] 8.00000000000001e-35 TIGR01720
TIGR01720   NRPS-para261
IPR010071 Amino acid adenylation domain (Domain)
[36-427] 9.49999999999983e-123 TIGR01733 [1058-1448] 3.39999999999996e-119 TIGR01733 [2560-2962] 1e-125 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[504-572] 1.10000029896148e-05 SM00823 [1524-1592] 5.20000089003336e-10 SM00823 [3045-3113] 4.39999967439747e-06 SM00823
SM00823   PKS_PP
IPR020845 AMP-binding, conserved site (Conserved_site)
[152-163] PS00455 [1177-1188] PS00455 [2688-2699] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
Vanco_00070 Vanco_00070   Vanco_00090 Vanco_00090
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