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Vanco_00070 : CDS information

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Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction6,956 / 8,914 / + [in whole cluster]
6,956 / 8,914 / + [in contig]
Location6956..8914 [in whole cluster]
6956..8914 [in contig]
TypeCDS
Length1,959 bp (652 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)putative ABC transporter ATP-binding protein
Gene
Gene (GenBank)abc
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
comment
A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、cluster報告論文なし。このORFに関する続報もなし。
Related Reference
ACC
R4SMX8
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)
comment
BLAST id93%
Amycolatopsis orientalis HCCB10007_(AORI_1477)

vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。

AORI_1477(gene nameなし): ATP-binding cassette, subfamily B

このORFに関しては配列解析のみ。
ACC
Q799B1
NITE
Balhi_00060
PmId
[17693715] The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. (J Mol Microbiol Biotechnol. , 2007)
[17823795] The ABC transporter Tba of Amycolatopsis balhimycina is required for efficient export of the glycopeptide antibiotic balhimycin. (Appl Microbiol Biotechnol. , 2007)
comment
BLAST id88%
Amycolatopsis balhimycina_abc
Putative ABC transporter ATP-binding protein

---
[PMID: 17693715](2007)
vancomycinにとても似たグリコペプチド系抗生物質balhimycinの生合成gene clusterの追加報告。
新たにVanS, VanR, VanY, Bbr, Pdh, Tba, Dahpを同定している。

Tba(651aa) : ABC transporter ATP-binding protein

Tbaのpromoter領域にregulator Bbrが結合することが確認されている。

---
[PMID: 17823795](2007)
配列解析から、ABC-transporter様遺伝子によってコードされるTbaはexporterであると示唆された。

E. coliで発現、精製されたTbaは、ATPase活性を有する活性型であることが確認された。

tba不活化mutantは生育やグリコペプチド耐性に影響しなかったが、細胞外balhimycin濃度が減少し、細胞内balhimycin濃度は10倍高くなった。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [putative ABC transporter ATP-binding protein]
MEVVLRFEGVDKSENDPDPWVTKVRKGTVRRVLAYFRPHIGKVALFVLVAVLESLIVVAT
PLLLKELIDGGIVKNDPGVVVLMASLTAGLAVLAAALALLSGYISGKIGEGITYDLRVQA
LDHVRRLPIAFFTRTQTGVLVGRLHTELIMAQQHFTGLLMAATSLVMVVVVLAELIYLSW
LVAVISLLLIPIFLVPWIRVGRAIQRRSIRLMDANTGLGGLLQERFNVQGAMLSKLFGRP
DEEMAEYADRAGKIREVGVSLSVWGRMAFVMMGLMASLATALVYGIGGGLVLAGAFQLGT
LVAIATLLSRLFGPITQLSGMQELAQTVVVSFSRVFELLDLKPLIQERPDAVELEKDVAP
DIEFEDVVFRYPTADEVSLASLEHLRAERERGEVSPDVLRGVNFHVRAGTLTALVGPSGA
GKSTITHLVSRLYDPNSGSVRVGGHDLRDLSFASLRDTVGVVSQDAYLFHNTIRENLLYA
RPTATEDELIEACKGAQIWDLIESLPLGLDTVSGDRGYRMSGGEKQRLAIARLLLKEPTI
VVLDEATAHLDSESEAAVQRALKTALRSRTSLVIAHRLSTIREADQILVIDGGRVRERGT
HDELLAEGGLYAELYRTQFADPANDAAKPEIEEAELDEPEPQPAIQPIGFGG
selected fasta
>putative ABC transporter ATP-binding/permease protein [putative ABC transporter ATP-binding protein]
ATGGAAGTGGTGTTGCGCTTCGAGGGGGTGGACAAGAGCGAGAACGACCCCGATCCCTGG
GTGACGAAGGTCCGCAAGGGGACGGTGCGCCGGGTACTCGCCTACTTCCGCCCGCACATC
GGCAAGGTGGCGCTCTTCGTTCTCGTCGCCGTGCTGGAGTCGCTCATCGTCGTCGCAACC
CCCTTGCTGCTGAAGGAACTCATCGACGGCGGCATCGTCAAGAACGATCCCGGGGTCGTG
GTCCTGATGGCCTCCCTCACGGCCGGACTCGCCGTGCTGGCCGCGGCGCTGGCGCTGCTG
TCCGGCTACATCTCCGGAAAGATCGGCGAGGGGATCACCTACGACCTCCGGGTGCAGGCG
CTCGACCACGTCCGGCGGCTGCCGATCGCGTTCTTCACCCGCACCCAGACCGGTGTCCTG
GTCGGCAGGCTGCACACCGAACTGATCATGGCGCAGCAGCATTTCACCGGTTTGCTGATG
GCCGCCACCAGTTTGGTCATGGTCGTGGTGGTGCTGGCCGAGCTGATCTACCTTTCGTGG
CTCGTCGCCGTCATCTCGCTGCTGCTGATCCCGATCTTCCTCGTGCCGTGGATCCGGGTC
GGGCGGGCGATCCAGCGGCGCAGCATCCGGCTGATGGACGCGAACACCGGCCTCGGCGGA
CTTCTCCAGGAGCGGTTCAACGTCCAGGGCGCGATGCTCTCCAAGCTCTTCGGCCGTCCG
GACGAGGAGATGGCCGAGTACGCGGACCGCGCCGGGAAGATCCGCGAGGTCGGCGTGAGC
CTTTCCGTCTGGGGCCGGATGGCCTTCGTGATGATGGGGCTGATGGCCTCGCTCGCCACG
GCGCTCGTCTACGGGATCGGCGGCGGGCTCGTGCTCGCGGGGGCCTTCCAGCTCGGCACG
CTGGTCGCCATCGCCACGCTGCTTTCGCGGCTGTTCGGGCCGATCACCCAGCTGTCCGGG
ATGCAGGAGCTCGCGCAGACCGTCGTGGTCAGCTTCTCCCGGGTCTTCGAGCTGCTCGAC
CTGAAGCCGCTGATCCAGGAGCGTCCCGACGCGGTCGAACTCGAAAAGGACGTGGCGCCG
GACATCGAGTTCGAGGACGTGGTGTTCCGCTATCCCACCGCGGACGAGGTCTCGCTGGCG
TCGCTGGAACACCTGCGCGCCGAGCGGGAGCGCGGGGAGGTGTCGCCGGATGTCCTGCGC
GGCGTGAATTTCCACGTGCGGGCCGGAACGCTCACCGCGCTCGTCGGCCCGTCCGGCGCG
GGGAAGAGCACCATCACCCACCTGGTGTCCCGGCTGTACGACCCGAACTCCGGAAGCGTC
CGCGTCGGCGGGCACGATCTGCGGGACCTCAGCTTCGCCTCGCTCCGCGACACGGTGGGC
GTGGTCAGCCAGGACGCCTACCTCTTCCACAACACGATCCGGGAGAACCTTCTCTACGCG
CGTCCGACAGCCACCGAGGACGAGCTGATCGAGGCGTGCAAGGGGGCCCAGATCTGGGAC
CTGATCGAATCCCTCCCGCTCGGGTTGGACACCGTCTCGGGTGATCGCGGCTACCGCATG
TCGGGCGGGGAGAAGCAGCGACTGGCCATCGCCAGGCTGCTGCTGAAGGAGCCGACGATC
GTCGTGCTCGACGAAGCCACCGCGCATCTGGACTCCGAATCGGAGGCCGCCGTCCAGCGG
GCGCTCAAGACCGCGCTGCGCAGCCGTACCTCGCTGGTGATCGCCCACCGGTTGTCCACG
ATCCGCGAGGCCGACCAGATCCTCGTGATCGACGGCGGCCGGGTGCGGGAGCGCGGGACG
CACGACGAGCTGCTGGCCGAAGGTGGCCTTTACGCCGAGCTCTACCGCACGCAGTTCGCC
GACCCGGCGAACGACGCGGCCAAGCCGGAAATCGAAGAAGCCGAGCTGGACGAGCCTGAG
CCTCAGCCGGCGATCCAACCCATTGGCTTCGGAGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[45-314] 8.19999999999997e-19 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[362-617] PS50893
PS50893   ABC_TRANSPORTER_2
[423-548] 9.9e-24 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[408-594] 4.49999046578037e-17 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[16-343] 2.19999900980707e-62 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[520-534] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[44-327] PS50929
PS50929   ABC_TM1F
SignalP No significant hit
TMHMM
[41-63] Transmembrane (i-o) [78-100] Transmembrane (o-i) [155-172] Transmembrane (i-o) [176-198] Transmembrane (o-i) [274-296] Transmembrane (i-o)
Transmembrane 5   
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