Vanco_00060 : CDS information

Location

Organism	Amycolatopsis orientalis
Strain	ATCC 19795 (=NBRC 12806)
Entry name	Vancomycin
Contig	HE589771
Start / Stop / Direction	5,919 / 6,758 / + [in whole cluster] 5,919 / 6,758 / + [in contig]
Location	5919..6758 [in whole cluster] 5919..6758 [in contig]
Type	CDS
Length	840 bp (279 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative prephenate dehydrogenase
Product (GenBank)	prephenatedehydrogenase (NADP+)
Gene
Gene (GenBank)	pdh
EC number	1.3.1.-
Keyword	Hpg
Note
Note (GenBank)
Reference	ACC G4V4Q8 comment A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、報告論文なし。
Related Reference	ACC Q799B2 NITE Balhi_00050 PmId [20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010) comment BLAST id82% Amycolatopsis balhimycina_pdh Putative prephenate dehydrogenase 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。 pdhは追加コピーによる過剰発現でも、deletion mutantでも、balhimycin産生に有意な変化は見られなかった。
	ACC Q04983 PmId [7916685] ( , ) comment BLAST id34% Zymomonas mobilis subsp. mobilis_tyrC Protein TyrC Alternative name(s): Arogenate dehydrogenase(ADH,EC 1.3.1.43) Prephenate dehydrogenase(PDH,EC 1.3.1.12) Cyclohexadienyl dehydrogenase E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。 B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。 tyrosineによるfeedback阻害は受けない。
	ACC R4SKG4 PmId [24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014) comment BLAST id88% Amycolatopsis orientalis HCCB10007_(AORI_1476) vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。 AORI_1476(pdh): Prephenate dehydrogenase このORFに関しては配列解析のみ。 Figure 5ではPdhとされていて、L-Hpgの生合成に割り当てられている。
Family/Domain	FD IPR003099 comment [IPR003099] Prephenate dehydrogenase (Domain) Molecular Function GO:0004665 prephenate dehydrogenase (NADP+) activity GO:0008977 prephenate dehydrogenase activity

Sequence

>putative prephenate dehydrogenase [prephenatedehydrogenase (NADP+)]
MTIEKVLVVGTGLIGTSVALALREKDVAVHLSDADARAAALARDLGAGREWAGEEVDLAL
IAVPPHLVGERLAALQKRGAARAYTDVASVKVDPIADAERLGCDLTSYVPGHPLAGREKS
GPAAARADLFLGRPWALCPGTETDADALRLTGELVSLCGAKAVTVDAREHDAAVALVSHA
PHVVASAMAASLTGGDDVALGLAGQGLRDVTRIAAGDPLLWRMILAGNALPVAGVLDRIA
GDLAAAASALRSGDLDEVTDLLRRGVAGQGRIPAGSPGR

>putative prephenate dehydrogenase [prephenatedehydrogenase (NADP+)]
GTGACCATCGAGAAGGTGCTTGTCGTCGGGACCGGGCTGATCGGTACCTCGGTCGCGCTG
GCCCTTCGCGAAAAGGACGTGGCGGTCCACCTCTCCGACGCCGACGCGCGGGCCGCGGCG
CTCGCACGGGACCTCGGTGCCGGCCGGGAATGGGCGGGCGAAGAGGTCGACCTGGCCCTG
ATCGCCGTTCCGCCGCACCTGGTGGGGGAGCGGCTGGCCGCGCTGCAGAAGCGAGGGGCC
GCTCGGGCTTACACCGACGTGGCCAGCGTCAAGGTCGATCCGATCGCCGACGCGGAACGG
CTCGGCTGCGACCTGACCTCTTACGTGCCCGGCCATCCGCTCGCCGGCCGGGAGAAATCC
GGACCGGCGGCGGCCCGCGCCGATCTGTTCCTCGGCCGTCCCTGGGCACTGTGCCCCGGC
ACGGAGACGGACGCGGATGCCTTGCGGCTGACCGGGGAACTGGTGTCGTTGTGCGGGGCG
AAGGCCGTCACGGTGGACGCGCGCGAGCACGATGCGGCGGTGGCGCTGGTCTCGCACGCC
CCGCATGTGGTGGCGTCGGCGATGGCGGCGAGCCTGACGGGTGGTGACGACGTCGCACTG
GGCCTGGCGGGGCAGGGATTACGGGACGTGACGCGGATCGCGGCCGGGGATCCCTTGCTG
TGGCGGATGATCCTCGCGGGGAACGCCCTGCCGGTCGCGGGAGTGCTCGACCGGATCGCG
GGCGATCTCGCGGCGGCGGCCTCGGCGTTGCGGTCCGGCGACCTGGACGAGGTGACGGAT
CTGCTGCGGCGCGGCGTCGCGGGACAAGGCCGGATCCCGGCGGGTTCCCCGGGACGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003099 Prephenate dehydrogenase (Domain) PS51176 PDH_ADH PF02153 PDH IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Vanco_00050 Vanco_00070