Vanco_00130 Vanco_00130   Vanco_00150 Vanco_00150

Vanco_00140 : CDS information

close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction40,162 / 41,382 / + [in whole cluster]
40,162 / 41,382 / + [in contig]
Location40162..41382 [in whole cluster]
40162..41382 [in contig]
TypeCDS
Length1,221 bp (406 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)P450 monooxygenase C
Gene
Gene (GenBank)oxyC
EC number
Keyword
  • 3rd cross-linking
  • AB ring
Note
Note (GenBank)
Reference
ACC
comment
A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、cluster報告論文なし。個々のタンパクなどでの続報もなし。
Related Reference
ACC
Q8RN03
PmId
[12888556] ( , )
comment
BLAST id100%
Amycolatopsis orientalis (Nocardia orientalis)_cyp165C4(oxyC)
Cytochrome P450 165C4 (Vancomycin biosynthesis protein OxyC)

G4V4R6とAA配列は全く同じ。

---
[PMID: 12207020](2002)
A. orientalis DSM40040からのoxyA, oxyB, and oxyCのクローニングとシークエンス。
OxyCに関しては配列解析のみ。

---
[PMID: 12888556](2003)
OxyCの結晶構造解析。
ACC
O87675
NITE
Balhi_00130
PmId
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)
comment
BLAST id88%
Amycolatopsis balhimycina_oxyC
P450 monooxygenase

---
[PMID: 12404385](2001)
oxyAと同様にoxyB, oxyCのmutantを作製し、その産物の構造から各oxygenaseの役割と順番を割り当てている。

1) OxyBによるC-O-D ring systemの形成
2) OxyAによるD-O-E ring systemの形成
3) OxyCによるbiaryl AB systemの形成

また、bicyclic C-O-D-O-E ring中間体は、1LeuのN-methylationと、4HpgのO-glycosylationの基質となれることもわかった。

---
[PMID: 16730832](2006)
oxyA, oxyB, oxyCの非極性mutantsを作製し、閉環反応の順番を再確認している。
また、これらmutant株をvancomycin産生菌A. orientalis DSM40040由来の対応するoxygenase genesで補完すると、balhimycin産生は回復した。
ACC
R4SZ79
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)
comment
BLAST id92%
Amycolatopsis orientalis HCCB10007_(AORI_1484)

vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。

AORI_1484(oxyC): Cytochrome P450

このORFに関しては配列解析のみ。
Figure 5ではOxyCとされ、架橋形成に割り当てられている。

close this sectionSequence

selected fasta
>putative cytochrome P450 [P450 monooxygenase C]
MGHDIDQVAPLLREPANFQLRTNCDPHEDNFGLRAHGPLVRIVGESSTQLGRDFVWQAHG
YEVVRRILGDHEHFTTRPQFTQSKSGAHVEAQFVGQISTYDPPEHTRLRKMLTPEFTVRR
IRRMEPAIQSLIDDRLDLLEAEGPSADLQGLFADPVGAHALCELLGIPRDDQREFVRRIR
RNADLSRGLKARAADSAAFNRYLDNLLARQRADPDDGLLGMIVRDHGDNVTDEELKGLCT
ALILGGVETVAGMIGFGVLALLDNPGQIELLFESPEKAERVVNELVRYLSPVQAPNPRLA
IKDVVIDGQLIKAGDYVLCSILMANRDEALTPDPDVLDANRAAVSDVGFGHGIHYCVGAA
LARSMLRMAYQTLWRRFPGLRLAVPIEEVKYRSAFVDCPDQVPVTW
selected fasta
>putative cytochrome P450 [P450 monooxygenase C]
ATGGGGCACGATATCGATCAGGTCGCGCCGCTCCTGCGGGAGCCGGCGAACTTTCAGCTG
AGGACGAACTGCGATCCGCACGAGGACAATTTCGGGCTCAGGGCGCACGGCCCTTTGGTG
CGGATAGTCGGCGAATCGTCCACCCAGCTGGGCAGGGACTTCGTCTGGCAGGCCCACGGT
TACGAGGTCGTGCGCAGGATATTGGGTGATCACGAACATTTCACGACGCGGCCGCAATTC
ACCCAGTCGAAATCCGGCGCCCATGTGGAGGCCCAGTTCGTCGGGCAGATCTCCACTTAT
GACCCACCCGAGCACACCCGTTTGCGGAAAATGCTCACGCCGGAGTTCACGGTCCGCCGG
ATCCGCCGGATGGAGCCCGCGATCCAAAGTCTCATCGATGATCGCCTCGATCTGCTGGAG
GCCGAGGGGCCGTCCGCGGACCTCCAGGGCCTGTTCGCCGATCCGGTCGGCGCGCACGCC
CTGTGCGAACTGCTCGGCATCCCGCGCGACGACCAGCGTGAGTTCGTCCGGCGGATCAGG
CGGAACGCCGACCTGAGCCGGGGACTCAAGGCACGGGCCGCCGACAGCGCGGCGTTCAAC
CGGTATTTGGACAACCTCCTCGCCAGGCAGCGCGCCGACCCCGACGACGGTCTCCTCGGC
ATGATCGTGCGGGACCACGGCGACAACGTCACGGACGAGGAACTGAAGGGCCTCTGCACG
GCGCTGATCCTCGGCGGTGTCGAAACCGTCGCCGGGATGATCGGTTTCGGTGTCCTCGCC
CTCCTGGACAATCCCGGACAGATCGAGTTGCTTTTCGAGTCCCCCGAAAAGGCCGAACGC
GTCGTCAACGAACTCGTCCGGTATCTGTCCCCGGTGCAGGCGCCGAATCCGCGTCTCGCC
ATCAAGGACGTGGTCATCGACGGGCAGCTGATCAAGGCGGGGGACTACGTCCTGTGCTCG
ATTCTCATGGCCAACCGGGATGAGGCGCTGACCCCGGATCCCGACGTCCTCGACGCGAAT
CGCGCCGCGGTGTCGGACGTCGGATTCGGGCACGGCATCCATTACTGCGTCGGCGCGGCC
TTGGCCAGATCCATGCTGCGCATGGCTTATCAGACTTTGTGGCGGCGATTCCCGGGACTG
CGGCTGGCAGTGCCCATCGAAGAAGTGAAGTACCGCAGCGCGTTCGTAGATTGCCCTGAT
CAGGTACCGGTCACCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[2-406] 1.1e-93 SSF48264
SSF48264   Cytochrome P450
[9-406] 2.4e-91 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
[279-377] 1.8e-12 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[100-111] 2e-47 PR00359 [147-163] 2e-47 PR00359 [164-179] 2e-47 PR00359 [201-223] 2e-47 PR00359 [280-291] 2e-47 PR00359 [298-325] 2e-47 PR00359 [326-341] 2e-47 PR00359 [347-356] 2e-47 PR00359 [356-367] 2e-47 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[349-358] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Vanco_00130 Vanco_00130   Vanco_00150 Vanco_00150
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