Vanco_00270 : CDS information

Location

Organism	Amycolatopsis orientalis
Strain	ATCC 19795 (=NBRC 12806)
Entry name	Vancomycin
Contig	HE589771
Start / Stop / Direction	56,142 / 57,494 / + [in whole cluster] 56,142 / 57,494 / + [in contig]
Location	56142..57494 [in whole cluster] 56142..57494 [in contig]
Type	CDS
Length	1,353 bp (450 aa)

Annotation

Category	4.3 transport
Product	putative transporter
Product (GenBank)	sodium hydrogen exchanger
Gene
Gene (GenBank)	ORF100
EC number
Keyword
Note
Note (GenBank)
Reference	ACC G4V4S9 comment A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、cluster報告論文なし。個々のタンパクなどでの続報もなし。
Related Reference	ACC R4SMZ9 PmId [24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014) comment BLAST id81% Amycolatopsis orientalis HCCB10007_(AORI_1497) vancomycinの大量産生に使用されているA. orientalis ATCC 43491由来工業用菌株HCCB10007の完全ゲノムシークエンス。そこからvcm cluster(AORI_1471-1505)を同定し、いくつかの遺伝子のmutantsを作製してin vivo機能を特徴づけている。 AORI_1497(gene nameなし): Antipoter このORFに関しては配列解析のみ。
Related Reference	ACC Q939X8 NITE Balhi_00270 PmId [21690280] Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. (Antimicrob Agents Chemother. , 2011) comment BLAST id80% Amycolatopsis balhimycina Putative antiporter orf7に相当するentry. balhimycin生合成gene cluster内でグリコペプチド耐性に関与するかもしれない遺伝子として、orf7, vnlR, vnlS, vanYbを同定。 orf7はNa+/H+ antiporter geneに50%以上の相同性あり。しかしorf7のin-frame deletion mutantは、balhimycinの産生と耐性に影響しなかった。
Family/Domain	FD IPR006153 comment [IPR006153] Cation/H+ exchanger (Domain) Molecular Function GO:0015299 solute:proton antiporter activity

Sequence

>putative transporter [sodium hydrogen exchanger]
MLLSLPAPIAPIAAHSLLLFLLQAGLLLLLAVTFGRLAARFGMPAVVGELFVGVILGPSL
LAWAAPGLHGWLFPAVAEQYHLLDAVGQIGVLLLVGLTGMQMDMRMVRRRFGAAAGVGLG
GLIIPLGLGIGAGFVLPKTLIPGGTDITVFALFLGVAMCVSAIPVIAKTLIDMNLLHRNV
GQLTLAAGMIDDVFGWLMLSVVSAMAVQAASAGTVLLSLAYLVAIVAFAFTLGRPLTRGV
LRIAGKSDGPWATVGTVVALLLLASAGTHALGMEALFGAFVCGILIGTAGKVDPAKLAPL
RTVILSVFAPLFFATAGLRMDLTALVRPEVLLTGLAVLLLAIIGKFVGVFAGARFSGLNK
WEGLALGAGLNARGVIGIMVATVGLRLGILGVEIYTIVILVAIVTPLMAPPILRFAMKRL
EQTAEEQVRETEQRAWSTQTTVSEQEQPLH

>putative transporter [sodium hydrogen exchanger]
GTGCTTCTCTCGCTTCCCGCGCCGATCGCGCCGATCGCCGCGCACAGTCTCCTGCTTTTC
CTGCTCCAGGCGGGTTTGCTGCTCCTGCTCGCGGTCACCTTCGGGCGGCTGGCCGCCCGG
TTCGGGATGCCCGCCGTCGTCGGCGAACTGTTCGTCGGGGTGATCCTCGGCCCGTCATTG
CTGGCCTGGGCAGCGCCGGGGCTGCACGGCTGGCTGTTCCCGGCCGTCGCGGAGCAGTAC
CACCTTCTCGACGCCGTCGGCCAGATCGGGGTCCTGCTGCTGGTCGGTCTCACCGGGATG
CAGATGGACATGCGCATGGTCCGGCGGCGGTTCGGTGCCGCCGCCGGGGTCGGCCTCGGT
GGCCTGATCATCCCGCTGGGGCTGGGGATCGGCGCGGGTTTCGTCTTGCCGAAGACGTTG
ATCCCCGGAGGCACGGACATCACCGTCTTCGCGTTGTTCCTCGGCGTGGCGATGTGCGTC
AGCGCCATCCCGGTCATCGCGAAGACGCTCATCGACATGAATCTGCTGCACCGCAACGTC
GGACAGCTCACGCTCGCGGCGGGCATGATCGACGACGTCTTCGGCTGGCTGATGCTGTCC
GTCGTCAGCGCGATGGCGGTCCAGGCGGCGAGCGCCGGAACGGTGCTGCTCTCCCTCGCG
TACCTGGTCGCCATCGTCGCCTTCGCCTTCACTCTCGGCAGACCGTTGACGCGAGGCGTG
CTGCGTATCGCCGGCAAGTCCGACGGTCCGTGGGCCACGGTCGGCACCGTCGTCGCCCTG
CTGCTCCTCGCCTCGGCCGGCACGCACGCCCTCGGGATGGAGGCCCTCTTCGGTGCCTTC
GTCTGCGGCATCCTGATCGGCACGGCGGGCAAGGTCGACCCGGCCAAGCTCGCCCCGCTG
CGCACCGTGATCCTGTCGGTGTTCGCGCCGCTCTTCTTCGCCACGGCCGGTTTGCGGATG
GACCTCACCGCGCTGGTCCGGCCGGAGGTCCTGCTCACCGGGCTGGCGGTGCTGCTGCTG
GCCATCATCGGCAAGTTCGTCGGAGTGTTCGCCGGTGCGCGGTTCAGCGGCCTGAACAAA
TGGGAAGGACTGGCGCTCGGCGCCGGGCTGAACGCGCGCGGGGTCATCGGGATCATGGTG
GCCACGGTCGGCCTGCGGCTGGGCATCCTCGGCGTGGAGATCTACACGATCGTCATCCTC
GTCGCGATCGTCACCCCGCTGATGGCGCCGCCGATCCTGCGGTTCGCGATGAAGAGACTC
GAGCAGACCGCCGAAGAACAGGTGCGCGAAACCGAACAGCGGGCCTGGAGCACGCAAACG
ACGGTGAGTGAACAGGAACAGCCGCTCCATTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006153 Cation/H+ exchanger (Domain) PF00999 Na_H_Exchanger
SignalP	No significant hit
TMHMM	Transmembrane 12

Vanco_00260 Vanco_00280