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Vanco_00360 : CDS information

close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction66,328 / 67,422 / + [in whole cluster]
66,328 / 67,422 / + [in contig]
Location66328..67422 [in whole cluster]
66328..67422 [in contig]
TypeCDS
Length1,095 bp (364 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative phospho-2-dehydro-3-deoxyheptonate aldolase
putative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
putative DAHP synthase
Product (GenBank)putative aldolase
Gene
Gene (GenBank)ald
EC number2.5.1.54
Keyword
  • Bht
  • Hpg
  • Dpg
Note
Note (GenBank)
Reference
ACC
comment
A. orientalis ATCC 19795でのvancomycin生合成 gene clusterとして配列が登録されているが、cluster報告論文なし。個々のタンパクなどでの続報もなし。
Related Reference
ACC
Q799B0
NITE
Balhi_00360
PmId
[20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010)
comment
BLAST id77%
Amycolatopsis balhimycina_ald
Phospho-2-dehydro-3-deoxyheptonate aldolase(EC:2.5.1.54)

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。

balhimycinのaglycone骨格となるアミノ酸(Bht, Hpg, Dpg)は、直接的または間接的にshikimate経路に由来する。shikimate経路の律速酵素Dahp synthaseをコードするdahpの追加コピーを発現した株は、グリコペプチド生産性が3倍に改善された。逆にdahpのdeletion mutant株はbalhimycin産生で有意な減少をもたらした。
ACC
Q6TZ04
PmId
comment
BLAST id60%
Amycolatopsis methanolica_aroF
Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)

DAHP-synthaseを2つ(aroG, aroF)もっている。それぞれを発現させ、活性をみたり、何により阻害されるかを確認している。
aroG(DS1:plant-type DAHP synthase[Q6TZ03])
aroF(DS2:E. coli-type DAHP synthase[Q6TZ04])
ACC
P0AB91
PmId
[1682314] ( , )
[8778789] ( , )
[12126632] ( , )
comment
BLAST id53%
Escherichia coli (strain K12)_aroG
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (EC:2.5.1.54)

close this sectionSequence

selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [putative aldolase]
MMTTIAAVDLDNQRIDRMVPLVTPALLHHELPLSATAADTVREGRETVVRVLNGEDDRLI
VIAGPCSIHDPAAALEYADRLAAIGARFVDDLVIVMRVYFEKPRTVGGWKGLINDPHLDG
SGDVNHGLRIARDLLLELAEGGLPTASEWLDTTIPAYLADTVSWGAIGARTVESQNHRML
ASGLSMPVGFKNRRDGDVTVAVDAIRAAEARHVVPGVDPSGLPAILHTVGNPDCHLVLRG
GNSAPNHYPASVRAALATLQNAGLTRRVVIDASHDNSRKDHLQQPVVAEQIAQQIRTGQR
GIAGVMLESNLKAGRQDLHPDQPLTYGQSITDACIDIAATQNVLQTLATATAARRRSGKR
ADDR
selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [putative aldolase]
GTGATGACAACCATTGCCGCTGTCGATCTCGACAACCAGCGCATCGACCGGATGGTCCCC
TTGGTCACTCCCGCCCTCCTGCACCACGAACTCCCGCTCAGCGCCACCGCGGCCGACACG
GTGCGGGAAGGCCGCGAAACCGTCGTCCGCGTCCTCAACGGTGAGGACGACCGGCTGATC
GTGATCGCCGGGCCCTGTTCCATCCACGACCCCGCCGCGGCACTCGAGTACGCCGACCGA
CTCGCCGCCATCGGCGCCCGGTTCGTCGACGACCTCGTGATCGTCATGCGCGTGTACTTC
GAAAAGCCGCGGACGGTCGGCGGCTGGAAGGGGCTCATCAACGATCCCCATCTCGACGGC
AGCGGCGACGTCAACCACGGGTTGCGCATCGCCAGGGATCTCCTTCTCGAACTCGCGGAA
GGCGGCCTGCCCACGGCGAGCGAATGGCTCGACACCACCATTCCGGCCTACCTCGCCGAC
ACCGTCTCCTGGGGCGCGATCGGCGCCCGCACCGTCGAAAGCCAGAACCACCGCATGCTC
GCCAGCGGACTGTCGATGCCCGTCGGCTTCAAGAACCGCCGTGACGGCGATGTCACCGTC
GCGGTCGACGCGATCCGTGCCGCCGAGGCACGTCATGTGGTGCCCGGCGTCGACCCCAGC
GGCCTGCCCGCGATCCTGCACACCGTCGGCAACCCCGACTGTCATCTCGTGCTGCGCGGC
GGGAACAGCGCGCCCAACCACTACCCCGCCTCGGTCCGCGCGGCGCTCGCCACGCTCCAG
AACGCCGGGCTGACCCGCCGGGTCGTGATCGACGCCAGCCACGACAACAGCCGGAAGGAC
CACCTCCAGCAGCCCGTCGTCGCCGAGCAGATCGCCCAGCAGATCAGGACCGGCCAGCGC
GGGATCGCCGGTGTCATGCTGGAGTCCAACCTCAAGGCGGGCCGCCAGGACCTGCACCCC
GACCAGCCGCTCACCTACGGCCAGTCGATCACCGACGCCTGTATCGACATCGCCGCCACC
CAGAACGTGCTCCAGACCCTCGCCACGGCGACCGCCGCCCGCCGCCGCAGCGGCAAGCGG
GCGGACGACCGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006218 DAHP synthetase I/KDSA (Domain)
[50-344] 1.29999999999998e-86 PF00793
PF00793   DAHP_synth_1
IPR006219 DHAP synthase, class 1 (Family)
[11-355] TIGR00034
TIGR00034   AroFGH
[2-359] PIRSF001361
PIRSF001361   DAHP_synthase
IPR013785 Aldolase-type TIM barrel (Domain)
[14-357] G3DSA:3.20.20.70
G3DSA:3.20.20.70   Aldolase_TIM
SignalP No significant hit
TMHMM No significant hit
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