Lasal2_00120 Lasal2_00120   Lasal2_00140 Lasal2_00140

Lasal2_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 31180
Entry nameLasalocid
Contig
Start / Stop / Direction13,216 / 15,954 / + [in whole cluster]
13,216 / 15,954 / + [in contig]
Location13216..15954 [in whole cluster]
13216..15954 [in contig]
TypeCDS
Length2,739 bp (912 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)LuxR-family transcriptional regulator
Gene
Gene (GenBank)las4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
comment
GenBank,Uniprotでは、NRRL3382Rと登録されている。

S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

las4: LuxR family regulator
homolog: LuxR family transcriptional regulator_S.avermitilis MA-4680

このORFはputative transcription regulator(belong to LuxR family)をエンコードすると推測された。機能解析はされていない。
Related Reference
ACC
B6ZK61
NITE
Lasal_00100
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。

lsd8: regulation
homolog: LuxR family transcriptional regulator_S.avermitilis MA-4680

lsd8はlasalocid 生合成遺伝子クラスタ-に存在する2つの転写因子のうちの一つ。機能解析はされていない。
ACC
Q9S0N8
NITE
Aver_00010
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
comment
Streptomyces avermitilis_aveR
LuxR-family transcriptional regulator

[PMID:10449723]
avermectin生合成gene clusterの報告。

AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

---
[PMID:19148632]abstract
aveR 不活化mutantは、avermectin中間体をいかなるavermectin派生体にも変換できない。よってAveRは、avermectinの生合成遺伝子と修飾遺伝子の両方の発現を調節するpositive regulatorであることが示唆された。
また、多量のaveRはavermectinの完全な損失をもたらすことから、aveRには最高許容限界濃度がある。

---
[PMID: 20012992]abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [LuxR-family transcriptional regulator]
MPFSVMESVEQQTEWRTLHTAFDDAASGISRTVLIEAGVACGKSTVLEGIGDYTAARGAL
VLHAVGSLSGSGTPLGVVRQLVEYPSFPATAGQNLLADVERGDYAQFSAALRNIAVRLPV
VVSVDDLHHVDAASLHCLLHLVGHTRRSRILTLFTQSLSCAPQDPTFDTELLRQPNFRRV
RLAPLDLHGVTAMLANHPEVDAGEDLTRELHSVSGGNPLLLRALIEDHLAARRFAGPSGE
RAEPGVAFSRAVLTCLHRGGSRMRDAATALAVLGESEHTALLGPLAGLSAAALAQARHAL
DATGITAEHRFRHRLARAAVLDSITPADRRALHLKAATLLHRHGASVREVAAHLLAGGGT
PDAWGVTALKEAAEEALAADRAQEAVAFLELAHAGSTDARTRAEITVRLACVMWRLNPAT
AESLYLAESLSALWSGLLPVPFIAPLANLLVAHGRLDEARHVLGRLQESTGPGQALPADT
EIMHPWEWDVHFPHALAPATPATSGRDIRLLSSHGGRPDPVHATGIERFLRVSPLTDTTL
TAVTTAIKSLIHHDRVDLAESWCDAFLQEAERRDAPGWEVLFASLRAEIALHQGRLTDAE
QYADRALAAVPEHTGVFFIGGPLSSLVLAYTAMGDYESAARRLNQSVPEALFKNVYGLRY
LRARGRYYLATNRAHTALGDFLDAGRLAARWGVDRPTLLPWRSDAAEAWLELGNPEQAER
LITDQLQMRDAINSRVRGISLRLRAMTADAKVRPRLLTKAVEALQTSGDRLEMARVLDAL
GDAQQALGDTTRAAAAKRRARHMARECGATAWGGAPQSTASRSVPASRRHWSAEGHAPSA
LTKLSESEKRVAALAACGYTNRDISAKLYITVSTVEQHLTRVYRKLHISRREQLPPDLQF
SPYENDCQSIAT
selected fasta
>putative LuxR family transcriptional regulator [LuxR-family transcriptional regulator]
GTGCCCTTCTCTGTCATGGAATCCGTTGAACAGCAAACAGAATGGCGTACGCTTCACACA
GCATTCGACGACGCGGCGTCCGGAATATCCCGGACGGTACTGATAGAAGCCGGAGTCGCC
TGCGGGAAAAGCACGGTTCTGGAGGGAATCGGCGACTACACCGCGGCCCGGGGCGCCTTG
GTCCTGCACGCCGTGGGCTCGTTGTCCGGCAGCGGAACACCGCTCGGCGTCGTACGCCAA
CTCGTCGAATACCCTTCATTCCCCGCAACCGCCGGCCAAAACCTCCTCGCGGACGTGGAA
AGGGGAGATTACGCACAGTTCTCCGCAGCGCTGCGGAATATAGCCGTCCGCTTACCGGTG
GTGGTGAGCGTAGACGACCTGCACCACGTGGACGCCGCCTCCTTGCACTGCCTCCTCCAC
CTCGTCGGCCACACCCGCCGGAGCAGGATTCTCACCCTGTTCACCCAGTCGTTGTCGTGC
GCTCCCCAAGACCCCACGTTCGACACCGAATTGCTGCGCCAGCCCAATTTCCGCAGGGTC
CGGCTCGCCCCGCTCGACCTGCACGGCGTGACCGCGATGCTCGCGAACCACCCCGAGGTC
GACGCCGGCGAGGACCTGACGCGGGAACTGCACTCCGTCAGCGGGGGGAACCCGCTGCTG
CTGCGCGCGCTGATCGAGGACCACCTGGCGGCCCGCCGCTTCGCGGGTCCGTCGGGTGAA
CGCGCCGAGCCCGGCGTCGCGTTCAGCCGGGCCGTGCTCACCTGTCTGCACCGGGGCGGC
AGCCGCATGCGTGACGCCGCCACCGCCCTGGCCGTTCTGGGCGAGTCCGAGCACACCGCC
CTGCTGGGCCCCCTTGCGGGCCTGTCGGCGGCGGCGCTCGCCCAGGCCCGGCACGCCCTC
GACGCCACCGGCATCACCGCGGAGCACCGCTTCCGCCACCGCCTGGCCAGAGCCGCCGTG
CTGGACAGCATCACCCCCGCGGACCGGCGCGCCCTGCACCTGAAGGCCGCGACCCTGTTG
CACCGGCACGGCGCCTCCGTCCGCGAGGTCGCCGCCCACCTGCTCGCCGGCGGCGGCACG
CCGGACGCCTGGGGAGTCACCGCACTCAAGGAGGCGGCCGAGGAGGCACTCGCCGCCGAC
CGGGCACAGGAGGCGGTCGCCTTCCTGGAGCTGGCCCACGCCGGTTCCACCGACGCCCGT
ACCCGGGCGGAGATCACCGTCCGGCTCGCCTGCGTCATGTGGCGCCTCAACCCGGCCACC
GCGGAGAGCCTGTACCTGGCGGAGTCCCTCTCCGCCCTGTGGTCCGGGCTGCTGCCGGTG
CCCTTCATCGCGCCGCTGGCCAACCTGCTCGTCGCCCACGGCCGCCTCGATGAGGCACGT
CACGTCCTCGGCCGGCTGCAGGAGTCGACCGGTCCCGGCCAGGCCCTGCCGGCGGACACC
GAGATCATGCACCCCTGGGAGTGGGACGTGCACTTCCCCCACGCCCTGGCGCCGGCCACT
CCGGCGACGTCCGGCCGGGACATCCGTCTGCTGAGCTCGCACGGCGGCCGGCCCGATCCC
GTCCACGCCACGGGGATCGAGCGGTTCCTGCGGGTGTCGCCGCTGACCGACACCACCCTC
ACCGCCGTCACCACGGCGATCAAGTCGCTCATCCACCACGACCGCGTGGACCTGGCCGAG
AGCTGGTGCGACGCCTTCCTGCAGGAGGCCGAGCGGCGCGACGCCCCCGGTTGGGAGGTG
CTGTTCGCCTCCCTGCGCGCCGAGATCGCGCTGCACCAGGGCCGGCTCACCGACGCCGAA
CAGTACGCCGACCGCGCGCTGGCGGCCGTCCCGGAGCACACCGGGGTGTTCTTCATCGGC
GGTCCCCTGTCCAGCCTGGTGCTGGCGTACACCGCGATGGGCGACTACGAGTCCGCGGCC
CGACGGCTCAACCAGTCGGTGCCCGAAGCGCTGTTCAAGAACGTGTACGGACTGCGCTAC
CTGCGCGCCCGGGGACGCTACTACCTGGCCACCAACCGGGCGCACACCGCCCTGGGCGAC
TTCCTCGACGCCGGCCGGCTCGCCGCGCGCTGGGGCGTCGACCGTCCCACGCTGCTGCCG
TGGCGCTCGGACGCCGCCGAGGCCTGGCTCGAACTGGGCAACCCGGAACAGGCCGAGCGG
CTGATCACCGACCAGCTCCAGATGCGCGACGCGATCAACTCCCGGGTGCGGGGCATCTCG
CTGCGGCTACGCGCGATGACCGCGGACGCCAAGGTGCGCCCACGACTGCTCACCAAGGCG
GTGGAAGCCCTGCAGACGTCCGGGGACCGGCTGGAGATGGCCCGCGTCCTGGACGCCCTG
GGCGACGCGCAGCAGGCCCTGGGCGACACCACCCGGGCCGCCGCGGCCAAGCGCCGGGCC
CGCCACATGGCCCGCGAGTGCGGAGCCACGGCGTGGGGCGGCGCTCCGCAGTCCACCGCC
TCCCGGTCTGTGCCGGCCTCACGGCGGCACTGGTCGGCCGAGGGACACGCGCCGTCCGCC
CTCACCAAGCTCAGCGAGTCGGAGAAGCGGGTGGCCGCCCTGGCCGCCTGCGGATACACC
AACCGCGACATATCGGCCAAGCTGTACATCACCGTCAGCACGGTCGAGCAGCACCTCACC
CGGGTCTACCGGAAGCTGCACATCTCACGCCGTGAACAGCTGCCGCCCGACCTCCAGTTC
AGCCCGTACGAGAACGACTGCCAGTCCATCGCCACGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[843-894] 4.5e-09 PF00196
PF00196   GerE
[844-858] 4.69999810630989e-08 PR00038 [858-874] 4.69999810630989e-08 PR00038 [874-886] 4.69999810630989e-08 PR00038
PR00038   HTHLUXR
[858-885] PS00622
PS00622   HTH_LUXR_1
[841-898] 5.59999989417023e-19 SM00421
SM00421   HTH_LUXR
IPR011990 Tetratricopeptide-like helical (Domain)
[549-852] 1.4e-17 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[853-894] 5e-16 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[822-894] 1e-13 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Lasal2_00120 Lasal2_00120   Lasal2_00140 Lasal2_00140
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