Lasal2_00180 Lasal2_00180   Lasal2_00200 Lasal2_00200

Lasal2_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 31180
Entry nameLasalocid
Contig
Start / Stop / Direction54,322 / 59,265 / + [in whole cluster]
54,322 / 59,265 / + [in contig]
Location54322..59265 [in whole cluster]
54322..59265 [in contig]
TypeCDS
Length4,944 bp (1,647 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)lasalocid modular polyketide synthase
Gene
Gene (GenBank)lasAIV
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase modules and related proteins
Reference
ACC
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
comment
GenBank,Uniprotでは、NRRL3382Rと登録されている。

S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

lasAIV: polyketide synthase KS, AT, KR, ACP

module7(KS, AT, KR, ACP)
Related Reference
ACC
B6ZK67
NITE
Lasal_00160
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。

lsd14: polyketide biosynthesis, module 7

module7(KS, AT, KR, ACP)

close this sectionPKS/NRPS Module

7 methylmalonyl-CoA
KS38..414
AT566..885
KR1201..1381
ACP1488..1563

close this sectionSequence

selected fasta
>polyketide synthase [lasalocid modular polyketide synthase]
MADVVNTEDRLRDYLKRATADLSRVRRQLQETEAASREPIAIIGMACRLPGGVDSPEGLW
ELVDSGTDAIAGFPLDRGWDVEGMYDPDAEAPGKTYVKEAGFLYDAGEFDAGFFGISPRE
AVSMDPQQRLMLEASWEAFERAGLDPARQRGTATGVFVGATATGYVSPAAEVPEGAEGFA
ITGNMTAVTSGRISYTLGLQGPAVTIDTACSSSLVALHLACQSLRQGECTTALAGGVTVM
PTPTAFTEFSRQRGLAPDGRCKSFAAAADGTNWAEGVAVLVVERLSDARRNGHRVLAVVR
GTAINQDGASNGLSAPNDLAQERVIRSALDNAGLTASDVDAVEAHGTGTTLGDPIEAQAL
LAAYGHERPAHRPLRVGSLKSNIGHAGPAAGVAGVIKMVMAMRHGVLPRSLHIDEPTPQV
DWSSGAVTLLTEPVDWPDSDRPRRAGVSAFGISGTNAHVILEQAPTQDPQQPAPPVPAAP
WLLSAKTPAALRAQARRLHTHLARHPHPDPTDIAHALATTRTPHEHRAALVTDDHGTRGP
ALAALAEGAPDACLISGTALSKGRTVFVFPGQGSQWTGMGRELLHTSPEFAAYIAECETA
LNDFVDWSLTDVLRGTEGAPGYDRVDVVQPALFAVMVSLARLWQHHGIHPDAVIGHSQGE
IAAAHIAGALSLQDAARIVALRSQALLPLAGLGGMTSLALPHDQALQLIQPWGQDLSIAS
VNGPHSTVVSGTTHALDELHHTCDTQGVRARRIPVDYASHSAQVESIRDTVFQAATGINP
QPTTIPLYSTVTGQPIDGTQLDADYWYTNLRHTVRFEETTRALLGSGHRHFIETTAHPVL
ALALEETIEATGSDARVTGTLRRDHGDLTQLHTALATAWTHGIDVDWTAVLGDRRTPFEL
PTYAFQRQRYWLEPGAGVGVPVGGTSAEARFWDAVEDEDLEALVAAIGADGDDASWAGVL
PALAGWRRRQREQSALDDLRYKVTWKPTAVADGASATGTWLVVVPESLAGGGWPVVVARA
VDQAGGRPVVLSVDAADGADRSRLGLRIHEALGEGPVPDAVVSLLALDPSALPGLPDVPQ
ALASTAALVQALLDLGLEARLWCVTSGAVSVSGADGPSAPEQAAVWGFGRVAGLEHPHLW
AGLVDLPPEADERTAARLVGVLAGAGGEDQVALRSSGVFVRRLVRAPASEVPAVRSWKPG
GTVLVTGGTGGLGRQVARWLARGGADHLLLVSRRGVDAPGADELVDELTDLGARVTVAAC
DVADRDAVQRLLSEQVPSDAPLTAVIHTAAVLDDGVIDSLSPERMEQVLRVKVGGAVHLY
ELTRESDLSAFVLFSSFGSTFGLPGLGNYAPGNAALEALAEQWRAEGRPATAVGWGTWAG
GGMADGGVGERGRTHGIHELEPALATAALEQALERDESSPVIIDIDWERFAVAFHAKRPT
RGFELVPEAQAALEAADGGPGPDGGAGDPSELLDRLAALPDAERDRALLEVVRGNAASVM
SHGAMRTATLEAVEPTRAFRDLGFDSLMAVELRNRIGAATGLRLAPTLVFDHPTPEAVVR
HLRAELGLEGDGAPDPVFDELDGLERALSSYTPDTDTRVKITKRLESLLWEWTRSEADAP
DPVDAADLAAVSDDEMFELIDRELGSA
selected fasta
>polyketide synthase [lasalocid modular polyketide synthase]
ATGGCAGACGTGGTGAACACCGAAGACCGACTTCGCGACTACCTGAAGCGGGCCACCGCC
GACCTGAGCAGGGTCCGCCGGCAGCTCCAGGAGACGGAGGCCGCGAGCCGCGAGCCGATC
GCGATCATCGGCATGGCCTGCCGGTTGCCCGGTGGCGTCGATTCCCCCGAGGGCCTGTGG
GAGCTCGTGGACTCCGGCACCGACGCCATCGCCGGGTTCCCGCTGGACCGTGGCTGGGAC
GTGGAGGGGATGTACGACCCCGACGCGGAGGCCCCCGGGAAGACGTACGTGAAGGAAGCG
GGATTCCTGTACGACGCTGGTGAGTTCGACGCCGGGTTCTTCGGCATCTCGCCGCGCGAG
GCCGTCTCCATGGACCCCCAGCAACGGCTGATGCTGGAGGCCTCGTGGGAGGCGTTCGAG
CGTGCCGGCCTGGACCCGGCGCGGCAAAGGGGAACCGCGACCGGTGTGTTCGTCGGCGCG
ACCGCCACCGGCTACGTGTCGCCCGCCGCCGAAGTGCCCGAGGGCGCCGAGGGGTTCGCC
ATCACCGGCAACATGACCGCGGTGACCTCGGGACGCATCTCCTACACGCTGGGCCTGCAG
GGGCCCGCGGTCACGATCGACACGGCTTGCTCCTCGTCCCTCGTGGCCCTGCACCTGGCC
TGCCAGTCACTGCGTCAGGGCGAGTGCACGACGGCGCTGGCCGGCGGCGTCACGGTGATG
CCGACTCCGACGGCGTTCACCGAGTTCAGCCGACAGCGCGGACTGGCCCCCGACGGCCGC
TGCAAGTCTTTCGCGGCCGCCGCCGACGGCACCAACTGGGCGGAGGGCGTGGCGGTCCTC
GTCGTCGAGCGCCTGTCGGACGCCCGGCGCAACGGGCACCGGGTGCTCGCCGTGGTGCGC
GGCACCGCGATCAACCAGGACGGGGCCAGCAACGGGCTGTCGGCCCCCAACGACCTCGCC
CAGGAACGCGTGATCCGCTCGGCGCTGGACAACGCCGGTCTCACCGCGAGCGACGTCGAC
GCGGTCGAGGCGCACGGCACGGGCACCACCCTGGGTGACCCGATCGAGGCGCAGGCCCTG
CTCGCCGCCTACGGTCACGAGCGCCCGGCGCACCGCCCCCTGCGCGTGGGCTCCCTTAAA
TCCAACATCGGTCACGCGGGGCCGGCCGCGGGTGTGGCCGGGGTGATCAAGATGGTGATG
GCGATGCGGCACGGCGTCCTGCCCCGCAGCCTCCACATCGACGAGCCGACCCCGCAGGTC
GACTGGTCCTCCGGCGCCGTCACCCTGCTCACCGAACCGGTCGACTGGCCCGACTCCGAC
CGCCCCCGCCGCGCAGGCGTCTCCGCCTTCGGCATCAGCGGCACCAACGCCCACGTCATC
CTCGAACAAGCCCCCACCCAAGACCCCCAGCAACCCGCCCCACCCGTACCCGCCGCACCC
TGGCTGCTCTCCGCCAAGACCCCGGCAGCACTGCGCGCCCAGGCCCGACGCCTGCACACC
CACCTCGCCCGCCACCCCCACCCCGACCCCACCGACATCGCCCACGCCCTCGCCACCACC
CGCACCCCCCACGAACACCGCGCCGCACTCGTCACCGACGACCACGGCACGCGGGGACCG
GCCCTGGCGGCTCTGGCCGAGGGGGCGCCCGACGCGTGCCTCATCAGCGGAACGGCGCTG
TCCAAGGGACGTACGGTGTTCGTGTTTCCGGGTCAGGGTTCGCAGTGGACCGGCATGGGA
CGCGAACTCCTCCACACCTCACCGGAGTTCGCCGCCTACATCGCCGAGTGCGAGACCGCG
CTCAACGATTTCGTGGACTGGTCGCTCACCGACGTCCTGCGCGGCACCGAAGGAGCCCCC
GGCTACGACCGCGTCGACGTCGTCCAGCCCGCCCTCTTCGCCGTCATGGTCAGCCTCGCC
CGCCTCTGGCAACACCACGGCATCCACCCCGACGCCGTCATCGGCCACTCCCAAGGCGAA
ATCGCCGCCGCCCACATCGCCGGAGCCCTGAGCCTGCAGGACGCCGCCCGCATCGTCGCC
CTGCGTTCCCAAGCCCTCCTCCCCCTCGCCGGCCTCGGCGGCATGACCTCCCTCGCCCTC
CCCCACGACCAAGCCCTCCAACTCATCCAGCCCTGGGGCCAGGACCTGTCCATCGCCTCC
GTCAACGGACCCCACTCCACCGTCGTCTCCGGCACCACCCACGCCCTCGACGAACTACAC
CACACCTGCGACACCCAAGGAGTTCGGGCCCGCCGCATCCCCGTCGACTACGCCTCCCAC
TCCGCCCAAGTCGAAAGCATCCGCGACACCGTCTTCCAAGCAGCCACCGGCATCAACCCC
CAGCCCACCACCATCCCCCTCTACTCCACCGTCACCGGCCAACCCATCGACGGCACCCAG
CTCGACGCCGACTACTGGTACACCAACCTCCGCCACACCGTCCGCTTCGAAGAAACCACC
CGCGCTCTGCTCGGCTCCGGCCACCGCCACTTCATCGAGACCACCGCCCACCCCGTCCTC
GCCCTCGCCCTGGAAGAAACCATCGAGGCCACCGGCTCCGACGCCCGCGTCACCGGCACC
CTCCGCCGCGACCACGGCGACCTCACCCAACTCCACACCGCCCTCGCCACCGCCTGGACC
CACGGCATCGACGTCGACTGGACCGCAGTCCTGGGCGACAGGCGGACGCCGTTCGAGTTG
CCGACGTACGCCTTCCAGCGTCAGCGGTACTGGCTGGAGCCTGGTGCGGGCGTGGGCGTT
CCCGTGGGCGGGACGTCGGCCGAGGCGCGCTTCTGGGACGCGGTGGAGGACGAGGATCTC
GAAGCGCTGGTGGCGGCCATCGGTGCGGACGGCGACGACGCCTCGTGGGCCGGGGTGTTG
CCTGCTCTGGCCGGCTGGCGCCGTAGGCAACGAGAGCAGTCGGCGCTCGACGACCTGCGC
TACAAGGTGACCTGGAAGCCCACGGCCGTGGCCGACGGCGCGTCGGCGACCGGCACATGG
CTGGTGGTCGTTCCCGAGTCGCTCGCGGGCGGTGGCTGGCCCGTGGTGGTCGCGCGCGCC
GTGGATCAGGCCGGTGGGAGGCCGGTCGTGCTGTCCGTGGACGCGGCGGACGGCGCCGAC
CGCTCGCGCCTCGGCCTGCGCATCCACGAGGCGCTGGGCGAGGGGCCGGTCCCGGACGCC
GTGGTGTCGTTGCTCGCCCTCGATCCGTCCGCCCTTCCCGGTCTCCCGGACGTCCCGCAG
GCGTTGGCGAGCACCGCGGCCTTGGTGCAGGCGTTGCTGGACCTCGGTCTGGAGGCACGC
CTGTGGTGCGTGACGAGCGGGGCGGTCTCCGTGTCCGGGGCGGACGGGCCCAGTGCTCCG
GAGCAGGCGGCCGTGTGGGGGTTCGGCCGTGTGGCCGGGTTGGAGCACCCGCACCTGTGG
GCGGGGCTGGTGGACCTGCCGCCGGAGGCCGACGAGCGGACGGCGGCCCGGCTGGTGGGT
GTGCTCGCCGGTGCCGGGGGCGAGGACCAGGTGGCGCTCAGGTCTTCGGGGGTCTTCGTA
CGACGACTCGTCCGCGCCCCGGCGTCCGAAGTGCCGGCGGTGCGGAGCTGGAAGCCCGGC
GGCACGGTGCTGGTGACGGGCGGCACCGGCGGGCTGGGCCGTCAGGTGGCGCGTTGGCTG
GCTCGGGGCGGCGCGGACCACCTGCTGCTGGTGAGCCGGCGGGGTGTCGACGCTCCGGGT
GCGGACGAACTGGTCGACGAGCTCACCGACTTGGGCGCCCGGGTCACGGTCGCGGCCTGC
GACGTCGCCGATCGGGACGCCGTGCAGCGGCTGTTGTCGGAACAGGTGCCGTCGGACGCG
CCGCTGACCGCGGTGATCCACACCGCGGCCGTCCTGGATGACGGGGTGATCGACTCGCTG
TCGCCCGAGCGGATGGAGCAGGTGCTCCGGGTGAAGGTGGGGGGCGCCGTCCACCTGTAC
GAGCTGACGAGGGAGTCGGACCTCTCCGCGTTCGTGCTGTTCTCGTCGTTCGGGTCGACG
TTCGGACTGCCGGGCCTCGGCAACTACGCCCCCGGGAACGCCGCCCTGGAGGCGCTGGCC
GAGCAGTGGCGGGCGGAGGGGCGACCGGCCACGGCCGTCGGCTGGGGGACCTGGGCCGGC
GGTGGCATGGCCGACGGCGGAGTCGGGGAGCGCGGGCGTACGCACGGCATCCACGAGCTG
GAGCCGGCGCTCGCCACCGCCGCTCTCGAACAGGCGCTGGAGCGGGACGAGTCGAGCCCG
GTGATCATCGACATCGACTGGGAGCGGTTCGCGGTGGCGTTCCACGCGAAACGGCCCACG
CGCGGTTTCGAGTTGGTGCCGGAGGCCCAGGCAGCCTTGGAAGCGGCGGACGGCGGCCCG
GGACCGGACGGCGGCGCGGGAGATCCGTCGGAACTCCTCGACCGGCTGGCCGCTCTGCCC
GACGCGGAGCGTGACCGGGCACTGCTGGAGGTCGTACGGGGCAACGCCGCCTCCGTGATG
AGTCACGGCGCGATGAGGACGGCGACGCTGGAGGCCGTCGAGCCGACGCGTGCCTTCCGG
GACCTCGGCTTCGACTCGCTCATGGCGGTGGAGCTGCGCAACCGCATCGGTGCGGCCACC
GGCCTGCGGCTGGCGCCGACACTGGTCTTCGACCACCCCACCCCGGAAGCGGTGGTACGC
CACCTGCGGGCCGAACTCGGCCTGGAGGGCGACGGCGCCCCCGATCCCGTCTTCGACGAA
CTCGACGGCCTGGAACGGGCGCTGTCGTCGTACACGCCGGACACCGACACCCGCGTGAAG
ATCACCAAGCGCCTGGAGTCCCTCCTGTGGGAGTGGACGCGCTCGGAAGCCGATGCACCG
GACCCGGTCGACGCCGCGGACCTCGCGGCCGTCTCCGATGACGAGATGTTCGAGCTCATC
GACAGGGAGCTCGGATCGGCCTGA
[7] KS38..414
[7] AT566..885
[7] methylmalonyl-CoA757..761
[7] KR1201..1381
[7] ACP1488..1563
[7] KS112..1242
[7] AT1696..2655
[7] methylmalonyl-CoA2269..2283
[7] KR3601..4143
[7] ACP4462..4689

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[557-689] 9.2000000000001e-75 G3DSA:3.40.366.10 [757-874] 9.2000000000001e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1521-1536] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1488-1563] PS50075
PS50075   ACP_DOMAIN
[1511-1562] 4.2e-11 PF00550
PF00550   PP-binding
[1480-1580] 1.89999859865865e-20 SSF47336
SSF47336   ACP_like
[1489-1566] 1.8e-18 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1201-1380] 6e-60 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[38-288] 3.39999999999996e-94 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[296-414] 7.10000000000008e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[566-885] 4.69999999999992e-105 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[7-38] 2.4000006166714e-08 SSF101173
SSF101173   Polyketide_synth_docking
[7-31] 3.1e-09 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[564-868] 8.19994104549873e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[691-756] 1.80000149287196e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[40-300] 2.99999999999998e-90 G3DSA:3.40.47.10 [301-467] 1.09999999999999e-62 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[30-412] 5.50001754266469e-101 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1200-1387] 1.40000000000001e-39 G3DSA:3.40.50.720 [1461-1472] 1.40000000000001e-39 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[201-217] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[568-865] 8.70005082121738e-123 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1489-1566] 9.5000102816722e-27 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[40-466] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1201-1381] 5.79999653854781e-55 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Lasal2_00180 Lasal2_00180   Lasal2_00200 Lasal2_00200
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