Teicp_00040 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	DSM 43866 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ632270
Start / Stop / Direction	4,070 / 5,413 / + [in whole cluster] 4,070 / 5,413 / + [in contig]
Location	4070..5413 [in whole cluster] 4070..5413 [in contig]
Type	CDS
Length	1,344 bp (447 aa)

Annotation

Category	4.4 resistance
Product	putative UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-lactate ligase
Product (GenBank)	MurF-like protein
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF8
Reference	ACC Q6ZZK4 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment [PMID: 15113000](2004) DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf8: MurF; D-Ala- D-Ala adding enzyme D-Ala-D-Ala ligaseをコードするorf8はたぶん細胞壁生合成に関与し、耐性遺伝子と関連付けられる。配列解析のみ。
Related Reference	ACC Q70B07 NITE Teicp2_00100 PmId [11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000) [14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004) [15500981] Glycopeptide resistance determinants from the teicoplanin producer Actinoplanes teichomyceticus. (FEMS Microbiol Lett. , 2004) comment [PMID: 11386360, 14702401] ATCC 31121株teicoplanin生合成gene clusterの同定論文。 tcp ORF1: D-Alanyl-D-alanine adding enzyme 配列解析のみ。 --- [PMID: 15500981](2004) house-keeping murF(murF1)と、tcp clusterに含まれるmurF(murF2 =tcp ORF1に相当)について調査。 UDP-N-acetyl-muramyl-L-Ala-D-Glu-D-Lysへ、MurF1はD-Ala-D-Ala または D-Ala-D-Lacを付加できるが、MurF2はD-Ala-D-Lacのみに低い活性がある。 7つのtcp genes(tcp1-7に相当)を導入されたS. coelicolorは、teicoplaninとA40926への耐性を獲得した。

Sequence

>putative UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-lactate ligase [MurF-like protein]
MIPLSLEAIAAVVGGTVEGDHTVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAG
QAGAVAVLGSRPTALPTVVVEDARTALQALAADVVARLRERLTLVGVTGSQGKTSTKDLL
AAVLSSAAPTIATKGNLNNELGVPLTMLRAGAATRFLVVEMGARRIGDLAKLTDLVPLDV
AVVLNVGEAHLGVFGSRAAIAEGKGELVRGLVPGGTAILNADDPRVVAMRSLTDGPVLTF
GHAEDADVRVRDLTLDRLGRPSFTLRTADATARVKLPIVGAHQALNAAAAVAAALSAGLT
LDAAAATLATASLTRWRLELRDIAGGVTLLNDSYNASPGSTRAALDALAAIEGGRRVAVL
GQMLELGDESEAEHRAVGEYAAARADVVVTVGEAVRPLAVGAGERAVAVADNGAAVDWLR
GHLAPGDVVLVKASRGARLDEVAAALS

>putative UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-lactate ligase [MurF-like protein]
ATGATCCCGCTCAGCCTCGAGGCGATCGCCGCTGTTGTCGGGGGGACGGTCGAGGGAGAC
CACACGGTGACGGTCACCGCGCCGGCCGTGCTCGACGGTCGGCAGGCCGAGCCGGGCGGC
CTCTTCGTCGCCTTCGCCGGCGAGCACGTCGACGGCCACGACTACGCCGGCCAGGCCGGC
CAGGCGGGCGCGGTCGCCGTGCTCGGCTCGCGGCCCACGGCGCTTCCCACCGTCGTCGTC
GAAGACGCCCGGACCGCGCTGCAGGCCCTCGCCGCCGACGTCGTGGCGCGGCTGCGTGAA
CGGTTGACGCTCGTCGGGGTGACCGGCTCCCAGGGCAAGACGAGCACCAAGGACCTGCTG
GCCGCCGTGCTGTCGAGCGCCGCGCCGACGATCGCCACCAAGGGCAACCTCAACAACGAG
CTCGGCGTGCCCCTCACCATGCTGCGCGCCGGTGCGGCCACCCGGTTCCTCGTCGTCGAG
ATGGGCGCCCGCCGCATCGGTGACCTCGCCAAGCTCACCGACCTGGTTCCGCTCGACGTC
GCCGTCGTCCTCAACGTCGGCGAGGCCCACCTCGGCGTGTTCGGATCGCGTGCGGCCATC
GCCGAGGGCAAGGGGGAGCTGGTACGAGGGCTGGTGCCCGGGGGTACCGCGATCCTCAAC
GCGGACGACCCCAGGGTGGTCGCGATGCGCTCGCTCACCGATGGCCCGGTGTTGACGTTC
GGGCACGCGGAAGACGCGGATGTGCGGGTGCGCGACCTGACCCTGGACCGGCTCGGCCGA
CCGTCCTTCACGCTGCGCACCGCGGACGCCACGGCCCGGGTCAAGTTGCCGATCGTGGGC
GCCCACCAGGCACTCAACGCCGCAGCCGCGGTGGCGGCGGCGCTTTCCGCCGGCCTGACG
CTCGACGCGGCCGCGGCCACCTTGGCCACCGCCTCACTGACGAGGTGGCGCTTGGAGCTA
CGTGACATCGCCGGCGGCGTGACACTGCTCAACGACTCCTACAACGCCAGCCCCGGGTCG
ACGCGAGCCGCGCTGGACGCCCTGGCCGCGATCGAGGGTGGCCGCCGCGTCGCCGTCCTC
GGCCAGATGCTGGAGCTGGGGGACGAGAGCGAGGCCGAGCACCGCGCCGTCGGGGAGTAC
GCCGCCGCCCGGGCCGACGTGGTGGTCACCGTCGGCGAGGCGGTTCGTCCGCTTGCCGTC
GGCGCGGGGGAGCGGGCGGTGGCAGTGGCGGACAACGGCGCGGCCGTCGATTGGCTTCGT
GGCCACCTCGCCCCCGGCGATGTGGTGCTCGTCAAGGCATCCCGTGGGGCGCGCCTCGAT
GAAGTCGCCGCCGCGCTGTCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000713 Mur ligase, N-terminal (Domain) PF01225 Mur_ligase IPR004101 Mur ligase, C-terminal (Domain) SSF53244 Mur_ligase_C PF02875 Mur_ligase_C G3DSA:3.90.190.20 Mur_ligase_C IPR005863 UDP-MurNAc-pentapeptide synthetase (Family) TIGR01143 MurF IPR013221 Mur ligase, central (Domain) G3DSA:3.40.1190.10 Mur_ligase_cen SSF53623 Mur_ligase_cen PF08245 Mur_ligase_M
SignalP	No significant hit
TMHMM	No significant hit

Teicp_00030 Teicp_00050