Teicp_00230 Teicp_00230   Teicp_00250 Teicp_00250

Teicp_00240 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction52,514 / 53,746 / + [in whole cluster]
52,514 / 53,746 / + [in contig]
Location52514..53746 [in whole cluster]
52514..53746 [in contig]
TypeCDS
Length1,233 bp (410 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)P450 monooxygenase
GeneoxyC
Gene (GenBank)
EC number
Keyword
  • 4th cross-linking
  • AB ring
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • ORF9*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf9*: P450 monooxygenase

遺伝子破壊実験がなされているbalhimycin生合成genesとの配列比較から、Orfs 5*, 7*, 9* はそれぞれ OxyA, OxyB, OxyC に関連があり、残基2-4, 4-6, 5-7間の架橋を担うと提唱している。
Related Reference
ACC
Q70AY6
NITE
Teicp2_00310
PmId
[11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000)
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
comment
DoBISCUIT上ではN末位置変更の結果、DSM 43866株でのtei orf9*とAA配列完全一致。

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[PMID: 11386360, 14702401]
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF22: OxyC, cross-linking aa 5-7

balhimycin生合成cluster gene(OxyC)と高い相同性があることから、AA5-7間の架橋形成が割り当てられている。

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[PMID: 25686610](2015)
SUPPLEMENTARY INFORMATION内のリストでATCC 31121株由来UniProt entryの記載があるが、同グループの他論文(PMID: 20974107, 25358800, 25586301)ではDSM43866株を使用してATCC 31121株由来のgene nameを採用している混乱があるため、同様の可能性あり。

X-domainの結晶構造解析や相互作用の実験から、X-domainがcytochrome P450(oxy genesでコードされる)による架橋形成に関与することがわかった。OxyA-CはX-domainと相互作用するが、OxyEはしない。OxyAとOxyBによる架橋形成には、X-domainを含む基質を必要とする。
ACC
Q8KLM1
NITE
A4793_00050
PmId
[17884639] The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of p450 mono-oxygenases to side chain cyclizations of glycopeptide a47934. (Chem Biol. , 2007)
comment
BLAST id81%
Streptomyces toyocaensis
StaJ
teicoplanin-type glycopeptide A47934生合成cluster gene

---
[PMID: 17884639](2007)
架橋形成に関するP450 monooxygenasesの調査。

mutants(ΔstaF, ΔstaG, ΔstaH, ΔstaJ）に蓄積した中間体の解析により、各架橋形成は特異的なoxygenaseへ明白に割り当てられ、環化の順番も決定された。
1) C-O-D by StaH
2) F-O-G by StaG
3) D-O-E by StaF
4) AB by StaJ

close this sectionSequence

selected fasta
>putative cytochrome P450 [P450 monooxygenase]
MDHDIDEVAPVVEPTANYMRRTVCDPHEDMFALRAHGPLIRIGGDAAVQMGADYLWQAMG
YDVVRQILGDHENFTTRLRLTASEPISGEGATKPQELVGQISTYDPPEHTRLRRMLTPEF
TVRRVRRLEPAIQELIDDRIDAMEAAGPPVDVQTVFADPVGGGALCELLGIPRDDRNQFM
RRVRQNVDLSRGYKARAADSAAFNRYLQSLITRQRKDPDEGFLGMLVRDHGDDITDEELK
GVCTALLLGGVETVAGMLGFGVLALLDHPDQRRLLFAGREEADRLVNELLRYLSAVQQPT
PRMAVKDVVVDGQQIRAGEYVLCSILMANRDEALTPHPNVLDAEREPAPHIGFGHGIHHC
IGAAVARTVLRMAYQTLWRRLPGLRLAIPAGQVKLRNAFIDSPDQLPVTW
selected fasta
>putative cytochrome P450 [P450 monooxygenase]
ATGGATCACGACATCGATGAGGTCGCGCCGGTCGTGGAGCCGACGGCGAACTACATGAGG
AGAACGGTCTGCGACCCGCACGAGGACATGTTCGCTCTGCGGGCGCACGGTCCGCTGATC
CGGATCGGCGGGGATGCCGCGGTCCAGATGGGCGCGGATTATCTCTGGCAGGCGATGGGC
TACGACGTCGTGCGGCAGATCCTCGGTGATCATGAGAACTTCACGACGCGGCTGCGGCTG
ACCGCGTCCGAACCGATCAGCGGGGAAGGCGCGACCAAACCGCAGGAGCTGGTCGGACAG
ATCTCGACCTACGATCCGCCCGAGCACACCCGCCTGCGCCGGATGCTCACGCCGGAGTTC
ACGGTCCGGCGCGTCCGCCGGCTGGAGCCCGCGATCCAGGAGCTCATCGACGACCGCATC
GATGCGATGGAGGCCGCGGGACCGCCCGTGGACGTGCAGACGGTCTTCGCCGATCCGGTC
GGCGGCGGCGCGCTGTGCGAACTGCTCGGCATCCCACGCGATGATCGGAACCAGTTCATG
CGCCGGGTCCGGCAGAACGTCGATCTCAGCCGCGGATACAAGGCCCGGGCGGCCGACAGC
GCGGCGTTCAACCGGTACCTGCAGAGCCTCATCACCCGGCAGCGCAAGGACCCCGACGAG
GGGTTCCTCGGCATGCTGGTGCGCGACCACGGCGACGACATCACGGACGAGGAGCTCAAG
GGCGTGTGCACGGCGCTGCTCCTCGGCGGTGTCGAGACCGTCGCGGGGATGCTGGGCTTC
GGGGTCCTCGCGCTGCTGGACCATCCCGACCAGAGGCGGCTGCTCTTCGCCGGCCGCGAG
GAGGCCGACCGCCTGGTCAACGAACTGTTGCGGTATCTGTCGGCGGTGCAGCAGCCGACT
CCGCGGATGGCCGTCAAGGACGTGGTCGTCGATGGGCAACAGATCAGGGCGGGGGAGTAC
GTGCTGTGCTCGATCCTCATGGCCAACCGGGACGAAGCGCTGACACCCCATCCCAACGTT
CTGGACGCGGAGCGTGAGCCGGCGCCACACATCGGATTCGGGCACGGCATCCATCACTGC
ATCGGGGCGGCGGTGGCGAGAACCGTGCTGCGGATGGCCTACCAGACCCTGTGGCGACGA
CTTCCCGGGCTGCGCCTCGCGATACCCGCCGGGCAGGTGAAGCTCAGGAACGCGTTCATC
GACAGTCCGGATCAGTTGCCGGTCACCTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[205-377] 3.9e-15 PF00067
PF00067   p450
[9-410] 5.5e-95 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
[10-410] 1.1e-89 SSF48264
SSF48264   Cytochrome P450
IPR002397 Cytochrome P450, B-class (Family)
[104-115] 3.6e-44 PR00359 [151-167] 3.6e-44 PR00359 [168-183] 3.6e-44 PR00359 [205-227] 3.6e-44 PR00359 [284-295] 3.6e-44 PR00359 [302-329] 3.6e-44 PR00359 [330-345] 3.6e-44 PR00359 [351-360] 3.6e-44 PR00359 [360-371] 3.6e-44 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[353-362] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Teicp_00230 Teicp_00230   Teicp_00250 Teicp_00250
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