Teicp_00290 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	DSM 43866 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ632270
Start / Stop / Direction	60,326 / 61,381 / + [in whole cluster] 60,326 / 61,381 / + [in contig]
Location	60326..61381 [in whole cluster] 60326..61381 [in contig]
Type	CDS
Length	1,056 bp (351 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative phospho-2-dehydro-3-deoxyheptonate aldolase putative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase putative DAHP synthase
Product (GenBank)	heptulosonate synthase
Gene
Gene (GenBank)
EC number	2.5.1.54
Keyword	Bht Hpg Dpg Tyr
Note
Note (GenBank)	ORF14*
Reference	ACC Q6ZZH9 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf14: DAHPS/Chorismate mutase Orf14は、7-phospho-2-dehydro-3-deoxy-D-arabinoheptulosonate synthetase (DAHPS) と chorismate mutaseの活性を持つdual-function enzymeのhomologである。 (S)-4-hydroxyphenylglycine (Hpg)合成における最初のステップはOrf14の活動に関与し、その後にshikimate経路の酵素が続き、chorismateを産生する。Orf14はchorismate → prephenateに変換する。配列解析のみ。
Related Reference	ACC Q70AY1 NITE Teicp2_00360 PmId [14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004) comment BLAST id99% --- ATCC 31121株teicoplanin生合成gene clusterの同定論文。 tcp ORF27: Chorismate mutase ORF27はAroA typeのputative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthaseをコードするが、cep clusterでコードされるものとは関係がない。配列解析のみ。
Related Reference	ACC Q9WYH8 PmId [12743122] ( , ) comment BLAST id55% Thermotoga maritima_aroF Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54) クローニングと大腸菌での過剰発現株の作製をし、諸性質決定を行っている。シキミ酸経路の最初のステップを担う。

Sequence

>putative phospho-2-dehydro-3-deoxyheptonate aldolase [heptulosonate synthase]
MVVVMAPEATSADVDAVVRLVESVGGQAFVSRGLSRTIVGLVGDVADFEALNLGSLAGVL
RVIRVSVPYKLVSREHHPARSVIRAGGVPIGPGTLTVIAGPCAVEVPGQTLEAARMARSA
GASMLRGGAFKPRTSPYSYQGLGERGLRILADVRAETGLAVVTEVIDPGSVDMVASYADM
LQIGARNMQNFALLQAVGSAGKPVLLKRGFSATIEEWLSAAEYIAQRGNLNIVLCERGIR
TFETATRNTLDISAVPVAQRLSHLPVIVDPSHSGGRRDLVVPLTRAAIAVGADGLLIDVH
PEPKTALCDGDQALPEADIREIADIVASLPAFLGREPAVPSGEFRHVPAAV

>putative phospho-2-dehydro-3-deoxyheptonate aldolase [heptulosonate synthase]
ATGGTCGTTGTGATGGCGCCGGAGGCCACTTCGGCGGATGTGGACGCGGTCGTCCGGCTG
GTCGAGTCGGTGGGCGGGCAGGCGTTCGTCAGCCGCGGGCTGTCCCGGACCATCGTCGGC
CTGGTCGGCGACGTGGCCGACTTCGAGGCGCTCAACCTGGGCAGCCTGGCGGGCGTGCTG
CGGGTGATCCGGGTGTCGGTGCCGTACAAGCTGGTCAGCCGGGAGCACCACCCGGCGCGC
TCGGTGATCCGGGCCGGCGGCGTGCCGATCGGTCCCGGCACGCTGACCGTGATCGCCGGG
CCGTGCGCGGTGGAGGTCCCCGGACAGACGCTGGAGGCGGCCCGGATGGCGCGCTCGGCC
GGGGCCTCGATGCTGCGCGGCGGGGCGTTCAAGCCGCGGACCTCACCGTACTCCTACCAG
GGGTTGGGGGAGCGGGGCCTGCGCATCCTGGCCGACGTGCGGGCCGAGACGGGGCTCGCC
GTGGTCACCGAGGTGATCGATCCCGGCAGTGTGGACATGGTCGCGTCGTACGCCGACATG
CTCCAGATCGGCGCCCGGAACATGCAGAATTTCGCGCTGCTGCAGGCGGTCGGGTCGGCC
GGCAAACCGGTGCTGCTCAAGCGCGGGTTCAGCGCCACGATCGAGGAATGGCTCAGCGCC
GCCGAGTACATCGCGCAGCGCGGCAATCTGAACATCGTGTTGTGTGAGCGCGGGATCCGG
ACGTTCGAGACCGCTACCCGTAACACGCTGGACATCAGCGCGGTGCCGGTCGCCCAGCGG
CTCTCCCACCTGCCGGTGATCGTGGATCCGTCGCACTCCGGCGGCCGCCGCGATCTGGTG
GTGCCGCTGACCCGCGCGGCGATCGCGGTGGGCGCGGACGGGTTGCTCATCGACGTCCAC
CCGGAGCCGAAGACCGCTCTGTGCGACGGTGACCAGGCGCTGCCGGAAGCCGACATCCGG
GAGATCGCCGACATCGTGGCGAGCCTGCCCGCGTTCCTGGGGCGGGAGCCGGCCGTGCCC
TCGGGTGAATTCCGACACGTTCCCGCCGCGGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006218 DAHP synthetase I/KDSA (Domain) PF00793 DAHP_synth_1 IPR006268 Phospho-2-dehydro-3-deoxyheptonate aldolase, subtype 2 (Domain) TIGR01361 DAHP_synth_Bsub IPR013785 Aldolase-type TIM barrel (Domain) G3DSA:3.20.20.70 Aldolase_TIM
SignalP	No significant hit
TMHMM	No significant hit

Teicp_00280 Teicp_00300