Teicp_00360 Teicp_00360   Teicp_00380 Teicp_00380

Teicp_00370 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction71,751 / 72,347 / + [in whole cluster]
71,751 / 72,347 / + [in contig]
Location71751..72347 [in whole cluster]
71751..72347 [in contig]
TypeCDS
Length597 bp (198 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative GTP cyclohydrolase 1
putative GTP cyclohydrolase I
Product (GenBank)GTP cyclohydrolase I
GenefolE
Gene (GenBank)
EC number3.5.4.16
Keyword
Note
Note (GenBank)
  • ORF22*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf22*: GTP cyclohydrolase I

配列解析から、Orf4, Orf22*, Orf25*はtyrosine生合成の代替ルートに関与し、tyrosineの高い需要を満たす助けとなると示唆されている。
Related Reference
ACC
Q6ZZH1
NITE
Teicp2_00440
PmId
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
comment
DSM 43866株でのtei orf22*とAA配列完全一致。
UniProt 同一entry。

---
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF35: GTP cyclohydrolase

ORF35はtype I GTP cyclohydrolasesに配列相同性を示す。配列解析のみ。
ACC
Q8GLS8
PmId
comment
BLAST id51%
Nocardia iowensis_gch(folE)
GTP cyclohydrolase 1 (EC:3.5.4.16)

GTP cyclohydrolase I (GCH)をコードするNocardia sp. NRRL 5646由来遺伝子gchをクローニング、異種性発現して精製し、そのタンパクの消化実験から、天然Nocardia GCHの活性型は10量体であると結論付けている。
ACC
P0A6T5
PmId
[1459137] ( , )
[12297008] ( , )
comment
BLAST id39%
Escherichia coli_folE
GTP cyclohydrolase 1 (EC:3.5.4.16)

HAMAP MF_00223 template
Family/Domain
FD
IPR001474
comment
[IPR001474] GTP cyclohydrolase I (Family)
Function; GTP cyclohydrolase I activity

UniProt Q6ZZH1登録ではHAMAP MF_00223 hitあり。

close this sectionSequence

selected fasta
>putative GTP cyclohydrolase 1 [GTP cyclohydrolase I]
MTVTAKHVGNTRPAPDLATATAAAATFLDALGVDLSPESLQETPARMARAYADLLTAREF
KLTTFDNDEGYDELVMARNIPIRSVCEHHLLPFIGVAHVGYLPAARILGLSKLARVVEMF
AHGTQVQERLTKQIADWLIKELEPNGVGVVIEAEHLCMTLRGVQAVGANTITSSMLGVLR
DDARSRGEFLAAAGLGGR
selected fasta
>putative GTP cyclohydrolase 1 [GTP cyclohydrolase I]
ATGACCGTCACGGCGAAGCACGTCGGGAACACCCGGCCGGCGCCCGACCTGGCGACCGCG
ACCGCGGCCGCGGCGACCTTCCTCGACGCGCTCGGCGTCGACCTGAGCCCGGAGAGCCTG
CAGGAGACCCCGGCCCGGATGGCCCGGGCGTACGCCGACCTGCTCACCGCCCGCGAGTTC
AAGCTCACCACGTTCGACAACGACGAGGGCTACGACGAGCTGGTGATGGCCCGCAACATC
CCGATCCGCTCGGTGTGCGAGCACCACCTGCTGCCCTTCATCGGCGTCGCGCACGTCGGC
TACCTGCCCGCCGCCCGCATCCTCGGACTGTCCAAGCTGGCCCGGGTCGTCGAGATGTTC
GCGCACGGCACCCAGGTGCAGGAACGGCTGACCAAGCAGATCGCCGACTGGCTGATCAAG
GAGCTGGAGCCGAACGGGGTGGGCGTGGTGATCGAGGCCGAGCACCTGTGCATGACGTTG
CGCGGGGTGCAGGCGGTCGGCGCGAACACCATCACCTCGTCCATGCTCGGCGTGCTGCGC
GACGACGCGCGGTCGCGCGGCGAGTTCCTGGCCGCCGCCGGGCTGGGCGGGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001474 GTP cyclohydrolase I (Family)
[24-192] 4.70000000000003e-58 TIGR00063
TIGR00063   FolE
IPR018234 GTP cyclohydrolase I, conserved site (Conserved_site)
[73-89] PS00859
PS00859   GTP_CYCLOHYDROL_1_1
IPR020602 GTP cyclohydrolase I/Nitrile oxidoreductase (Domain)
[92-177] 4.70000000000003e-34 PF01227
PF01227   GTP_cyclohydroI
SignalP No significant hit
TMHMM No significant hit
Teicp_00360 Teicp_00360   Teicp_00380 Teicp_00380
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