Teicp_00360 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	DSM 43866 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ632270
Start / Stop / Direction	70,393 / 71,754 / + [in whole cluster] 70,393 / 71,754 / + [in contig]
Location	70393..71754 [in whole cluster] 70393..71754 [in contig]
Type	CDS
Length	1,362 bp (453 aa)

Annotation

Category	4.3 transport
Product	putative transporter
Product (GenBank)	Na+-H+ antiporter
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF21*
Reference	ACC Q6ZZH2 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf21: Na(+)-H(+) antiporter Orf21は多くのion transportersに似ている。類似遺伝子が他のglycopeptide gene clustersにも同定されているが、その機能は確かでない。配列解析のみ。
Related Reference	ACC Q6ZZH2 NITE Teicp2_00430 PmId [14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004) comment DSM 43866株でのtei orf21*とAA配列完全一致。 UniProt同一entry. --- ATCC 31121株teicoplanin生合成gene clusterの同定論文。 tcp ORF34: Integral membrane transporter ORF34はABC-type transporterをコードすると予測されるので、排出機能に特定されそうである。配列解析のみ。
Related Reference	ACC Q939X8 NITE Balhi_00270 PmId [21690280] Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. (Antimicrob Agents Chemother. , 2011) comment BLAST id57% Amycolatopsis balhimycina Putative antiporter orf7に相当するentry. balhimycin生合成gene cluster内でグリコペプチド耐性に関与するかもしれない遺伝子として、orf7, vnlR, vnlS, vanYbを同定。 orf7はNa+/H+ antiporter geneに50%以上の相同性あり。しかしorf7のin-frame deletion mutantは、balhimycinの産生と耐性に影響しなかった。
Family/Domain	FD IPR006153 comment [IPR006153] Cation/H+ exchanger (Domain) Molecular Function GO:0015299 solute:proton antiporter activity

Sequence

>putative transporter [Na+-H+ antiporter]
MSFEAPPSSLGGHALLAFLIAVATLLLVAHLLARLAERVRMPAVVGELATGVILGPSLLG
HLLPGLTDWVFPPGTDQMHLLDAVGQIGILLLVGLTGTHLDVAMVRRRKATAARISLGGL
LLPLALGVGVGYQLAGWLSGAVVGDRLVFAGFMGVAMCVTAIPVIAKTLSDMRLLHRDVG
QMTLAAGTIDDAVGWFLLSVVSMAATAGLTTGHVVQAVAYLLGFVVLAVLLGRPLVRRVM
RLADRGEGSGSSIVTAVVVVLTGAAITQALGMEPVFGAFVAGILVGLPAAANQAKLAALR
TVVLSVLAPLFLATAGFRMDLTALADPQVAIAAVLVLAIAIVGKFAGAYAGARLSRLSRW
EGLAIGAGMNSRGVVEVVVALTGLRLGVLNTATYTIVVLVAIVTSVMAPPLLRYACARIA
QNEDERLRKLHHDTWNGVPAGAGQSPSPAGEQA

>putative transporter [Na+-H+ antiporter]
ATGTCCTTCGAAGCGCCGCCGTCGTCACTGGGCGGCCATGCCCTGCTCGCTTTCCTGATC
GCGGTGGCGACGCTGCTGCTGGTCGCCCACCTGCTGGCCCGCCTGGCGGAACGGGTGCGG
ATGCCGGCCGTCGTGGGTGAGCTGGCCACCGGCGTCATCCTGGGCCCGTCCCTGCTGGGC
CACCTGCTGCCCGGACTGACCGACTGGGTCTTCCCGCCCGGCACCGACCAGATGCACCTG
CTCGACGCGGTAGGTCAGATCGGCATCCTGCTCCTGGTCGGACTCACCGGCACCCACCTG
GACGTCGCCATGGTGCGGCGGCGCAAGGCCACCGCCGCGCGGATCAGCCTGGGCGGCCTG
CTGCTGCCGCTGGCTCTCGGGGTCGGCGTCGGCTACCAGCTGGCCGGCTGGCTCAGCGGC
GCCGTGGTCGGTGACCGGCTGGTCTTCGCCGGATTCATGGGCGTCGCGATGTGCGTGACA
GCCATCCCGGTGATCGCCAAAACCCTCTCCGACATGCGGCTGCTGCACCGCGACGTGGGC
CAGATGACGCTGGCGGCGGGCACCATCGACGACGCGGTCGGCTGGTTCCTGCTGTCCGTG
GTGTCGATGGCCGCGACCGCCGGGCTCACCACCGGACACGTCGTCCAGGCGGTCGCCTAC
CTGCTCGGCTTCGTCGTCCTCGCGGTTCTGCTCGGCCGGCCGCTGGTCCGCCGCGTCATG
CGGCTGGCCGACCGCGGCGAGGGATCCGGCTCCAGCATCGTCACCGCGGTCGTCGTCGTC
CTGACCGGCGCGGCGATCACCCAGGCGCTGGGCATGGAGCCGGTCTTCGGCGCCTTCGTG
GCGGGCATCCTGGTCGGGCTGCCCGCGGCGGCGAACCAGGCGAAGCTGGCGGCGCTGCGC
ACGGTCGTGCTCAGCGTGCTGGCGCCGCTCTTCCTCGCCACGGCCGGCTTCCGGATGGAC
CTGACCGCGCTGGCCGACCCGCAGGTCGCGATCGCCGCGGTGCTCGTGCTGGCCATCGCC
ATCGTCGGCAAGTTCGCCGGGGCGTACGCCGGGGCCCGCCTCAGCCGGCTGTCCCGCTGG
GAGGGCCTGGCGATCGGCGCCGGGATGAACTCGCGCGGCGTGGTCGAGGTGGTGGTGGCG
CTCACCGGCCTGCGACTGGGCGTGCTGAACACCGCCACCTACACGATCGTCGTCCTGGTC
GCCATCGTCACCTCGGTGATGGCCCCGCCGCTGCTGCGCTACGCCTGTGCCCGCATCGCC
CAGAACGAGGACGAGCGGCTGCGCAAGCTGCACCACGACACCTGGAACGGCGTCCCGGCC
GGCGCCGGCCAGTCCCCCTCACCCGCAGGAGAGCAGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006153 Cation/H+ exchanger (Domain) PF00999 Na_H_Exchanger
SignalP	No significant hit
TMHMM	Transmembrane 12

Teicp_00350 Teicp_00370