Teicp_00380 Teicp_00380   Teicp_00400 Teicp_00400

Teicp_00390 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction73,867 / 74,985 / + [in whole cluster]
73,867 / 74,985 / + [in contig]
Location73867..74985 [in whole cluster]
73867..74985 [in contig]
TypeCDS
Length1,119 bp (372 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative prephenate dehydrogenase
Product (GenBank)prephenate dehydrogenase
Genepdh
Gene (GenBank)
EC number1.3.1.-
Keyword
  • Hpg
Note
Note (GenBank)
  • ORF24*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf24*: Prephenate dehydrogenase

配列解析のみ。(S)-4-hydroxyphenylglycine (Hpg)合成を触媒する4つの酵素のうちのひとつ。prephenate dehydrogenase (Pdh)はshikimate一次代謝経路と関係があり、prephenate → 4-hydroxyphenylpyruvateに変換する。
Related Reference
ACC
Q799B2
NITE
Balhi_00050
PmId
[20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010)
comment
BLAST id51%, C末足りない
Amycolatopsis balhimycina_pdh
Putative prephenate dehydrogenase

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。

pdhは追加コピーによる過剰発現でも、deletion mutantでも、balhimycin産生に有意な変化は見られなかった。
ACC
Q04983
PmId
[7916685] ( , )
comment
BLAST id31%, C末足りない
Zymomonas mobilis subsp. mobilis_tyrC
Protein TyrC

Alternative name(s):
Arogenate dehydrogenase(ADH,EC 1.3.1.43)
Prephenate dehydrogenase(PDH,EC 1.3.1.12)
Cyclohexadienyl dehydrogenase

E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。

B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。
tyrosineによるfeedback阻害は受けない。
ACC
P20692
PmId
[4321765] ( , )
comment
BLAST id30%
Bacillus subtilis (strain 168)_tyrA
Prephenate dehydrogenase(EC:1.3.1.12)
C末にACT domainあり

strain 168, 23のclude extractsを用いて、prephenate DH活性確認。
cofactor: NAD
inhibitor: Tyr
Family/Domain
FD
IPR003099
comment
[IPR003099] Prephenate dehydrogenase (Domain)

Molecular Function
GO:0004665 prephenate dehydrogenase (NADP+) activity
GO:0008977 prephenate dehydrogenase activity

close this sectionSequence

selected fasta
>putative prephenate dehydrogenase [prephenate dehydrogenase]
MRTLLVIGTGLIGTSVALAARRAGVAVFLADRDVAAARVAEALGAGLAEPPRRPVDLALV
AVPPAQVGPVLRDAQANRAAHSYTDVAGVKGQPERDVLRLASDPASYVGGHPMAGRERSG
PLAATADLFAGKTWVLTPNERTGAAALERATTLAEICGAAPVRLDSRAHDTVVALTSHVP
HLMASLTAARLLHGPAATEALVGQGVRDVTRIAAGDPALWTDIVRSNAPAVAAVLRGVRD
DLTRLLAATDILAGHHPGGRAQAEHTVTDLLERGVAGAARTRPPAGAPEGFAVLRVVLDG
RPGSLARLLEAVAAHGGSARRAGAAPGPDAALAVDVELPAAGADAAAAALAAAGWAVSRP
GLASAARRGPHR
selected fasta
>putative prephenate dehydrogenase [prephenate dehydrogenase]
GTGCGCACTCTTCTCGTGATCGGAACCGGACTGATCGGTACGTCGGTGGCGCTGGCCGCG
CGTCGCGCCGGGGTGGCGGTGTTCCTCGCCGACCGCGACGTCGCCGCGGCCCGGGTCGCC
GAGGCGCTCGGCGCGGGCCTGGCCGAGCCGCCCCGCCGGCCGGTGGATCTCGCGCTCGTC
GCGGTGCCGCCCGCCCAGGTCGGCCCGGTGCTGCGGGACGCGCAGGCCAATCGCGCGGCG
CACAGCTACACCGACGTGGCCGGGGTCAAGGGGCAGCCGGAACGCGACGTCCTGCGGCTG
GCCAGCGACCCGGCGTCGTACGTCGGCGGGCACCCGATGGCCGGGCGGGAACGTTCCGGC
CCGCTGGCCGCCACGGCCGACCTGTTCGCCGGCAAGACCTGGGTGCTCACCCCGAACGAG
CGCACCGGCGCCGCGGCGCTGGAGCGCGCCACCACGCTCGCCGAGATCTGCGGTGCGGCG
CCGGTGCGGCTGGACAGCCGGGCGCACGACACCGTGGTGGCGCTGACCTCGCACGTCCCG
CATCTGATGGCCAGCCTCACCGCGGCCCGCCTGCTGCACGGGCCGGCCGCCACCGAGGCG
CTGGTGGGCCAGGGGGTCCGTGACGTCACCCGGATCGCGGCCGGCGACCCGGCGCTGTGG
ACCGACATCGTCCGGTCGAACGCGCCGGCGGTCGCCGCCGTGCTGCGCGGCGTCCGCGAC
GACCTCACCCGGCTCCTCGCCGCCACCGACATCCTGGCCGGCCACCACCCGGGCGGGCGG
GCCCAGGCCGAGCACACCGTCACCGACCTGCTGGAGCGAGGCGTCGCCGGGGCGGCCCGG
ACCCGGCCGCCCGCCGGGGCGCCGGAGGGCTTCGCCGTGCTCCGGGTGGTCCTCGACGGC
CGCCCCGGCAGCCTGGCCCGCCTGCTGGAGGCGGTCGCGGCGCACGGCGGCAGCGCGCGC
CGGGCCGGCGCGGCGCCCGGACCGGATGCCGCCCTGGCCGTCGATGTGGAGCTACCGGCG
GCCGGGGCCGACGCGGCGGCGGCCGCGCTCGCCGCGGCCGGCTGGGCGGTCAGCCGCCCC
GGCCTCGCCTCCGCGGCGCGCCGAGGGCCGCACCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003099 Prephenate dehydrogenase (Domain)
[2-282] PS51176
PS51176   PDH_ADH
[16-245] 1.5e-56 PF02153
PF02153   PDH
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[165-248] 2.60000517657733e-21 SSF48179
SSF48179   6DGDH_C_like
IPR016040 NAD(P)-binding domain (Domain)
[2-170] 3.39999999999999e-25 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-19] 0.627 Signal
Eukaryota   
[1-19] 0.498 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Teicp_00380 Teicp_00380   Teicp_00400 Teicp_00400
Page top