Teicp_00400 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	DSM 43866 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ632270
Start / Stop / Direction	76,780 / 75,242 / - [in whole cluster] 76,780 / 75,242 / - [in contig]
Location	complement(75242..76780) [in whole cluster] complement(75242..76780) [in contig]
Type	CDS
Length	1,539 bp (512 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	nonheme iron dioxygenase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF25*
Reference	ACC Q6ZZG8 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf25: Nonhaem iron dioxygenase 配列解析から、Orf4, Orf22, Orf25*はtyrosine生合成の代替ルートに関与し、tyrosineの高い需要を満たす助けとなると示唆されている。
Related Reference	ACC Q7WZ63 NITE A4092_00340 PmId [16499611] A derivative of the glycopeptide A40926 produced by inactivation of the beta-hydroxylase gene in Nonomuraea sp. ATCC39727. (FEMS Microbiol Lett. , 2006) comment BLAST id50% Nonomuraea sp. ATCC 39727_dbv28 Putative beta-hydroxylase ORF28はputative hydroxylaseをコードする。ORF28のmutantはtyrosine部分でbeta-OH基を欠いているA40926のdesoxyderivativeを産生したので、ORF28がBht残基の形成に実際に関与することが実証された。また、このmutantに遊離Bhtを与えてもA40926産生は回復しないため、NRPS module 6の基質がBhtではなく遊離Tyrである可能性が示唆されるが、chlorinated Bhtである可能性も除外はできていない。

Sequence

>putative oxidoreductase [nonheme iron dioxygenase]
MGVNEPVRLRSNAIIEPLVDRFYAWLHTVAPVPAALHLADFQVPLLESYLRDPRARAAAG
AGPAPTGGHLAELDETGGMEIAALLDGIKRDRADMLGLAAAVTEAEDLLQRYGRTPPHPG
LPAALHGLVQPVSGPGRQPAIRVLESLAYDSRYYDERRQSVRLSLDDGAERPGILGTPRL
PRPGALDLAVPFRHAGLDELFAARLRPTTLSRLREALELDDTRAGRLRALLTTEPGTAPE
RHLDAGGRIRFFGHACLLFQTPEAAVITDPFISADNRHGDRYTFDDLPDHIDLAVITQGR
PDHVVLETLLQLRSRIGAMVVPRSSRGNLHDPSIGRCLRALGFPVIEVDDFDEVPFPGGR
VVAAPFLDEHGEPDVRAKSTYLVQLAGASVFVGADSSGVDPVAYRLMRRQLGRVDLAFLG
MRRDNAPPARPAGQTRGRSGCDARQAAAIMQELGADEGYVYVMGEQPWQRHVMAAAGHRD
PGRRTQIDEFLGWCRERGITAERLLHRREWRW

>putative oxidoreductase [nonheme iron dioxygenase]
ATGGGCGTCAACGAGCCGGTCCGGCTCCGCTCCAACGCGATCATCGAGCCACTGGTCGAC
CGGTTCTACGCCTGGTTGCACACCGTGGCCCCGGTCCCGGCGGCCCTGCACCTCGCCGAT
TTCCAGGTCCCGCTGCTGGAGTCGTACCTGCGCGATCCGCGGGCGCGCGCGGCGGCCGGG
GCCGGCCCGGCGCCGACCGGCGGACACCTCGCCGAGCTCGACGAGACAGGCGGCATGGAG
ATCGCCGCGCTGCTGGACGGCATCAAGCGCGACCGGGCCGACATGCTCGGGCTCGCGGCC
GCCGTGACCGAGGCCGAGGACCTGCTGCAGCGGTACGGCCGCACGCCGCCGCACCCGGGG
CTGCCCGCCGCGCTGCACGGCCTGGTCCAGCCGGTGTCCGGCCCGGGCCGGCAGCCGGCG
ATCCGCGTCCTCGAATCGCTGGCATACGACAGCCGGTACTACGACGAGCGCCGGCAGTCG
GTACGGCTGTCGCTCGACGACGGCGCCGAGCGGCCCGGCATCCTCGGCACGCCACGGCTG
CCCCGGCCCGGGGCGCTCGACCTGGCGGTCCCGTTCCGGCACGCCGGCCTGGACGAACTC
TTCGCGGCCCGGCTGCGGCCCACCACGTTGTCGCGGCTGCGCGAGGCGCTGGAGCTCGAC
GACACCCGGGCCGGCCGGCTGCGGGCGCTGCTGACCACCGAGCCCGGCACCGCACCGGAG
CGGCACCTCGACGCGGGTGGCCGGATCCGCTTCTTCGGGCACGCCTGCCTGCTGTTCCAG
ACGCCGGAGGCCGCGGTGATCACCGATCCGTTCATCAGCGCCGACAACCGGCACGGTGAC
CGGTACACCTTCGACGACCTGCCGGACCACATCGACCTGGCGGTCATCACGCAAGGCCGC
CCGGACCACGTGGTGCTGGAGACGCTGCTGCAACTACGCTCCCGGATCGGCGCGATGGTG
GTTCCCCGGTCCTCGCGCGGCAACCTGCACGATCCGTCGATCGGCCGGTGTCTCAGGGCG
CTGGGCTTCCCGGTGATCGAGGTGGACGACTTCGACGAGGTGCCGTTCCCCGGTGGCCGG
GTGGTCGCCGCGCCGTTCCTGGACGAGCACGGCGAGCCGGACGTCCGCGCCAAGTCGACG
TACCTGGTGCAGCTGGCCGGGGCGAGCGTCTTCGTCGGCGCCGACTCCTCGGGCGTCGAT
CCGGTGGCGTACCGCTTGATGCGCCGGCAGCTCGGCCGGGTGGACCTCGCGTTCCTCGGT
ATGCGACGGGACAACGCGCCACCGGCCCGGCCGGCGGGCCAGACGCGCGGGCGTTCCGGC
TGCGACGCACGGCAGGCCGCCGCGATCATGCAGGAGCTCGGCGCGGACGAGGGCTACGTC
TACGTCATGGGGGAACAGCCCTGGCAGCGTCACGTCATGGCGGCCGCCGGCCACCGGGAC
CCGGGCCGCCGGACGCAGATCGACGAGTTCCTCGGCTGGTGCCGGGAGCGCGGCATCACC
GCCGAGCGCCTGCTGCACCGACGCGAGTGGCGCTGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR019204 Domain of unknown function DUF2070, membrane (Domain) PF09843 DUF2070
SignalP	No significant hit
TMHMM	No significant hit

Teicp_00390 Teicp_00410