Teicp_00410 Teicp_00410   Teicp_00430 Teicp_00430

Teicp_00420 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction77,365 / 78,192 / + [in whole cluster]
77,365 / 78,192 / + [in contig]
Location77365..78192 [in whole cluster]
77365..78192 [in contig]
TypeCDS
Length828 bp (275 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productputative siderophore-interacting protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF27*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf27*: Hypothetic protein

配列解析のみ。遺伝子を特徴づけられるデータはなかった。
Related Reference
ACC
A5F660
PmId
[8083157] ( , )
comment
BLAST id28%
Vibrio cholerae serotype O1_viuB
Vibriobactin utilization protein ViuB

V.cholerae_viuA(ferric vibriobactin receptor gene)の下流に位置するviuBのクローニングとシークエンス解析。
In vitro　transcription-translationにより、タンパク発現を確認。
viuB deletion mutantを作製し、Arnow assayなどによりシデロフォア合成の様子を確認。相補試験も行っている。
-->mutantでは正常なシデロフォアを形成するが、外因性のferric vibriobactin資化性が欠失。
・V.choleae viuB in E.coli fes mutant --> 相補可
・E.coli fes in V.choleae viuB mutant --> 相補不可
鉄イオン高/低濃度条件下でのNorthern解析により、viuBは鉄による負の制御を受ける。<--転写レベル
Family/Domain
FD
IPR013113, IPR007037
comment
[IPR013113] FAD-binding 9, siderophore-interacting (Domain)
[IPR007037] Siderophore-interacting protein (Domain)

close this sectionSequence

selected fasta
>putative siderophore-interacting protein [hypothetical protein]
MSGHVLAAHRPIVALSARRLTRIAPRMTRLVADGPGVRRFVPDGCTDAFVRVVFPRPGVR
YPPGLDPRRIRAELPRADWPRTRTYTVRRLDPVAAELTIDFVDHGPAGLAPPWLAALRPG
DPVLVQDAGGRYRPDPRAGWHLLAGDETALPAIAVTLAALAPRARAVALVEVDTAADELP
LCSAADVDLRWVHRCRGGDLVEAVRDLRFAPGAVQAFVHGEGGAVQHLRRHLLRDRAVAP
RLLSASGYWRRGLDDEQWRARKAAMASPVPAAGGR
selected fasta
>putative siderophore-interacting protein [hypothetical protein]
ATGTCCGGACACGTATTGGCCGCGCACCGTCCGATCGTCGCATTGTCGGCCCGGCGGCTC
ACCAGGATCGCGCCGAGAATGACCAGGCTGGTCGCGGATGGGCCGGGGGTCCGCCGATTC
GTGCCGGACGGCTGCACCGACGCCTTCGTCCGCGTCGTCTTCCCCCGGCCCGGCGTGCGC
TACCCGCCCGGCCTGGACCCGCGCCGGATCCGCGCCGAGCTGCCCCGCGCGGACTGGCCC
CGCACCCGCACCTACACCGTGCGGCGCCTCGACCCGGTGGCCGCCGAACTGACCATCGAC
TTCGTCGACCACGGCCCGGCCGGGCTCGCCCCGCCGTGGCTGGCCGCGCTGCGGCCCGGC
GACCCGGTCCTGGTCCAGGACGCCGGCGGCCGGTACCGGCCGGACCCGCGGGCCGGCTGG
CACCTGCTGGCCGGCGACGAGACGGCGCTGCCGGCCATCGCCGTGACGCTGGCCGCCCTG
GCGCCGCGGGCCCGGGCGGTGGCCCTGGTGGAGGTGGACACCGCCGCCGACGAGCTGCCG
CTGTGCAGCGCCGCCGATGTCGACCTGCGCTGGGTGCACCGGTGCCGGGGCGGCGATCTG
GTCGAGGCGGTGCGCGATCTGCGGTTCGCGCCCGGCGCGGTCCAGGCGTTCGTGCACGGC
GAGGGCGGGGCCGTCCAGCACCTGCGCCGCCACCTGCTGCGGGACCGCGCGGTGGCGCCG
CGGCTGCTGTCGGCCTCCGGGTACTGGCGCCGCGGGTTGGACGACGAGCAGTGGCGCGCG
CGCAAGGCCGCGATGGCGTCGCCGGTCCCCGCCGCGGGCGGCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007037 Siderophore-interacting protein (Domain)
[138-252] 4.9e-33 PF04954
PF04954   SIP
IPR013113 FAD-binding 9, siderophore-interacting (Domain)
[18-131] 9.20000000000002e-31 PF08021
PF08021   FAD_binding_9
IPR017927 Ferredoxin reductase-type FAD-binding domain (Domain)
[7-135] PS51384
PS51384   FAD_FR
IPR017938 Riboflavin synthase-like beta-barrel (Domain)
[18-138] 8.89999852234942e-07 SSF63380
SSF63380   Riboflavin_synthase_like_b-brl
SignalP No significant hit
TMHMM No significant hit
Teicp_00410 Teicp_00410   Teicp_00430 Teicp_00430
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