A4793_00020 A4793_00020   A4793_00040 A4793_00040

A4793_00030 : CDS information

close this sectionLocation

Organism
StrainNRRL 15009
Entry nameA47934
Contig
Start / Stop / Direction4,963 / 4,151 / - [in whole cluster]
4,963 / 4,151 / - [in contig]
Locationcomplement(4151..4963) [in whole cluster]
complement(4151..4963) [in contig]
TypeCDS
Length813 bp (270 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productsulfotransferase
Product (GenBank)StaL
GenestaL
ORF32
Gene (GenBank)
EC number2.8.2.-
Keyword
Note
Note (GenBank)
  • sulfotransferase
Reference
ACC
PmId
[12060705] Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. (Proc Natl Acad Sci U S A. , 2002)
[16492565] Biosynthesis of sulfated glycopeptide antibiotics by using the sulfotransferase StaL. (Chem Biol. , 2006)
[17329243] Crystal structure of StaL, a glycopeptide antibiotic sulfotransferase from Streptomyces toyocaensis. (J Biol Chem. , 2007)
[22753479] Sulfonation of glycopeptide antibiotics by sulfotransferase StaL depends on conformational flexibility of aglycone scaffold. (Proc Natl Acad Sci U S A. , 2012)
comment
[PMID: 12060705](2002)
Streptomyces toyocaensis NRRL15009由来A47934生合成gene clusterの同定論文。

34のORFsからなるclusterは、A47934の生合成とその調節に必要だと予測される遺伝子をすべて含む。グリコペプチド耐性を担う酵素をコードするORFsも含む。

StaL: Sulfotransferase

配列解析のみ。

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[PMID: 16492565](2006)
staLを置換したmutantはdesulfo-A47934を産生し、A47934を産生しない。精製したStaLを用い、様々な基質でのsulfotransferase活性を調査。StaLは、desulfo-A47934, teicoplanin aglycone, teicoplaninを硫酸化した。StaLによって硫酸化された物質は、わずかに抗菌活性が増加した。

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[PMID: 17329243](2007)
StaLと、cofactorの産物である3'-phosphoadenosine 5'-phosphate (PAP) とStaLの複合体の、結晶構造解析。配列解析、分子モデリング、重要残基の同定などもしている。

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[PMID: 22753479](2012)
StaL, cofactor産物 PAP, desulfo-A47934 aglycone基質から成る三元複合体の結晶構造解析。

close this sectionSequence

selected fasta
>sulfotransferase [StaL]
MNGMCWIASYPKAGGHWLRCMLTSYVTGEPVETWPGIQAGVPHLEGLLRDGEAPSADPDE
QVLLATHFTADRPVLRFYRESTAKVVCLIRNPRDAMLSLMRMKGIPPEDVEACRKIAETF
IADEGFSSVRIWAGEGSWPENIRSWTDSVHESFPNAAVLAVRYEDLRKDPEGELWKVVDF
LELGGRDGVADAVANCTLERMREMEERSKLLGLETTGLMTRGGKQLPFVGKGGQRKSLKF
MGDDIEKAYADLLHGETDFAHYARLYGYAE
selected fasta
>sulfotransferase [StaL]
ATGAACGGGATGTGCTGGATCGCCTCCTACCCGAAGGCCGGAGGCCACTGGCTCCGGTGC
ATGCTGACGTCCTACGTCACCGGCGAGCCGGTCGAGACCTGGCCGGGCATCCAGGCCGGG
GTGCCGCACCTCGAAGGGTTGTTGCGTGACGGGGAGGCCCCCTCCGCCGACCCGGACGAG
CAGGTCCTGCTGGCGACGCACTTCACCGCGGACCGCCCGGTGCTGCGGTTCTACCGGGAG
TCCACCGCCAAGGTGGTCTGCCTGATCCGGAACCCGCGCGACGCGATGCTCAGCCTGATG
CGGATGAAGGGGATTCCGCCGGAGGACGTCGAGGCCTGCCGGAAGATCGCCGAGACGTTC
ATCGCCGACGAGGGATTCTCGTCGGTGCGCATCTGGGCCGGCGAAGGATCATGGCCGGAG
AACATACGCAGTTGGACGGACTCGGTGCACGAGAGCTTCCCGAACGCCGCCGTCCTCGCG
GTGCGGTACGAGGACCTTCGGAAGGACCCCGAAGGGGAGCTGTGGAAGGTCGTGGACTTC
CTCGAACTCGGTGGACGGGACGGTGTGGCGGACGCGGTGGCGAACTGCACCCTGGAGCGG
ATGCGGGAGATGGAGGAGCGGAGCAAGCTCCTCGGCCTGGAGACGACGGGACTGATGACC
CGTGGGGGAAAGCAGCTGCCTTTCGTGGGCAAGGGCGGGCAGCGGAAGTCGCTGAAATTC
ATGGGCGACGACATCGAGAAGGCCTATGCGGACCTCCTCCATGGGGAAACCGATTTCGCC
CACTATGCCCGTCTCTATGGATACGCTGAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000863 Sulfotransferase domain (Domain)
[6-206] 4.80000000000001e-17 PF00685
PF00685   Sulfotransfer_1
SignalP No significant hit
TMHMM No significant hit
A4793_00020 A4793_00020   A4793_00040 A4793_00040
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