A4793_00230 A4793_00230   A4793_00250 A4793_00250

A4793_00240 : CDS information

close this sectionLocation

Organism
StrainNRRL 15009
Entry nameA47934
Contig
Start / Stop / Direction56,213 / 55,059 / - [in whole cluster]
56,213 / 55,059 / - [in contig]
Locationcomplement(55059..56213) [in whole cluster]
complement(55059..56213) [in contig]
TypeCDS
Length1,155 bp (384 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative prephenate dehydrogenase
Product (GenBank)Pdh
Genepdh
ORF10
Gene (GenBank)
EC number1.3.1.-
Keyword
  • Hpg
Note
Note (GenBank)
  • Prephenate dehydrogenase
Reference
ACC
PmId
[12060705] Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. (Proc Natl Acad Sci U S A. , 2002)
comment
Streptomyces toyocaensis NRRL15009由来A47934生合成gene clusterの同定論文。

34のORFsからなるclusterは、A47934の生合成とその調節に必要だと予測される遺伝子をすべて含む。グリコペプチド耐性を担う酵素をコードするORFsも含む。

Pdh: Prephenate dehydrogenase

配列解析のみ。ORFs 8-11は4-hydroxyphenylglycine(HPG)生合成で機能すると予想される。
Related Reference
ACC
Q04983
PmId
[7916685] ( , )
comment
BLAST id31%
Zymomonas mobilis subsp. mobilis_tyrC
Protein TyrC

Alternative name(s):
Arogenate dehydrogenase(ADH,EC 1.3.1.43)
Prephenate dehydrogenase(PDH,EC 1.3.1.12)
Cyclohexadienyl dehydrogenase

E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。

B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。
tyrosineによるfeedback阻害は受けない。
Family/Domain
FD
IPR003099
comment
[IPR003099] Prephenate dehydrogenase (Domain)

Molecular Function
GO:0004665 prephenate dehydrogenase (NADP+) activity
GO:0008977 prephenate dehydrogenase activity

close this sectionSequence

selected fasta
>putative prephenate dehydrogenase [Pdh]
MMRAMAVIGTGLIGTSVALAASRQGVVVYLSDRNPSAARTAAALGAGLTQTPAERVDLAV
LAVPPSQVGTVLTEVQKRELAVSYTDVASVKAGPEQVALAQAPDPSRYIGGHPLAGREQS
GPLAARAELFRDRPWALTPSPMTSNATFNRALELVALCGAVPVVTQSQAHDDAVALTSHV
PHLLASLMAARLRDAPAEMSRLAGNGLRDVTRIAGGDSRLWGDILQSNAGAVVGVVKRVH
EDLTRLVAALDQLAERGGEDRDDGLRTMMELLDRGVAGLAEIPGRRVGGGGRAVRVTVRD
RPGELPRLLGVVADFGVVPEDATVDADEAGALTVDFTVPPGAADRMVAELRADGWEAVRP
NGIGPYFREMARKEDGIPSEAVSK
selected fasta
>putative prephenate dehydrogenase [Pdh]
ATGATGCGCGCCATGGCCGTCATCGGCACGGGTCTGATCGGTACCTCGGTCGCGCTCGCG
GCAAGTCGGCAGGGAGTGGTGGTGTATCTGTCCGACCGGAACCCGTCTGCGGCCAGGACG
GCCGCCGCACTCGGTGCGGGACTCACCCAGACTCCCGCGGAGCGGGTGGACCTGGCCGTG
CTCGCCGTACCGCCGAGCCAGGTCGGCACGGTGCTCACGGAGGTGCAGAAACGCGAGCTC
GCCGTCAGCTACACCGACGTGGCGAGCGTGAAGGCCGGGCCGGAACAGGTCGCGTTGGCC
CAGGCTCCGGATCCGTCCCGCTACATCGGGGGTCACCCGCTGGCGGGCCGGGAGCAGTCG
GGCCCGCTGGCGGCCCGTGCCGAACTGTTCCGGGACCGCCCGTGGGCGCTCACCCCGTCG
CCGATGACGTCCAACGCGACCTTCAACCGGGCCCTGGAACTGGTGGCGCTGTGCGGCGCG
GTGCCGGTGGTGACCCAGAGCCAGGCACACGACGACGCGGTTGCCCTCACCTCCCACGTA
CCGCATCTGCTGGCGAGCCTGATGGCGGCGCGGCTGCGGGACGCCCCGGCCGAGATGTCC
AGGCTCGCGGGTAACGGACTGCGCGACGTCACCCGGATCGCGGGCGGTGACTCACGCCTG
TGGGGCGACATCCTCCAGTCCAACGCGGGGGCCGTGGTGGGTGTCGTGAAGCGCGTGCAC
GAGGACCTGACCCGGCTGGTGGCCGCCCTGGACCAGCTCGCCGAGCGGGGCGGCGAGGAC
CGGGACGACGGCCTGCGGACGATGATGGAGCTGCTCGACCGGGGTGTCGCCGGGCTTGCC
GAGATCCCCGGGCGCCGCGTCGGCGGCGGTGGGAGGGCCGTACGTGTGACCGTCCGGGAC
CGTCCCGGGGAGCTGCCGCGGCTGCTGGGCGTCGTCGCGGACTTCGGCGTCGTCCCCGAG
GACGCCACCGTCGACGCCGACGAGGCCGGCGCGCTCACCGTGGACTTCACCGTCCCGCCG
GGGGCCGCCGACCGGATGGTGGCCGAGCTCAGGGCCGATGGATGGGAGGCGGTCCGGCCG
AACGGCATCGGACCGTACTTCAGGGAAATGGCGCGAAAAGAAGACGGTATCCCGTCGGAA
GCCGTGTCTAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003099 Prephenate dehydrogenase (Domain)
[18-252] 6.09999999999996e-61 PF02153
PF02153   PDH
[3-283] PS51176
PS51176   PDH_ADH
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[166-274] 4.00000300869883e-22 SSF48179
SSF48179   6DGDH_C_like
IPR016040 NAD(P)-binding domain (Domain)
[5-172] 4.6e-25 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-20] 0.645 Signal
Eukaryota   
[1-20] 0.638 Signal
Bacteria, Gram-positive   
[1-20] 0.685 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
A4793_00230 A4793_00230   A4793_00250 A4793_00250
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