A4793_00280 A4793_00280   A4793_00300 A4793_00300

A4793_00290 : CDS information

close this sectionLocation

Organism
StrainNRRL 15009
Entry nameA47934
Contig
Start / Stop / Direction62,227 / 63,393 / + [in whole cluster]
62,227 / 63,393 / + [in contig]
Location62227..63393 [in whole cluster]
62227..63393 [in contig]
TypeCDS
Length1,167 bp (388 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productputative glycyltransferase
Product (GenBank)StaO
GenestaO
vanK
ORF5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • FemABX homolog
Reference
ACC
PmId
[12060705] Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. (Proc Natl Acad Sci U S A. , 2002)
comment
Streptomyces toyocaensis NRRL15009由来A47934生合成gene clusterの同定論文。

34のORFsからなるclusterは、A47934の生合成とその調節に必要だと予測される遺伝子をすべて含む。グリコペプチド耐性を担う酵素をコードするORFsも含む。

StaO: FemABX homologue

配列解析のみ。staOは、pentaglycine intermuramyl chain peptideの産生を担うfemABX familyに高い相同性のあるタンパクをコードする。
Related Reference
ACC
Q9XAK9
PmId
[15632111] ( , )
comment
BLAST id79%
Streptomyces coelicolor_SCO3593
Uncharacterized protein

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[PMID: 15130128](2004)
誘導性で高レベルなvancomycin耐性を与えるvanSRJKHAXの特徴づけ。
VanKはこの株においてvancomycin耐性に必須である。

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[PMID:15632111](2005)
同じグループによる続報。
VanKは、D-Ala-D-Alaの代わりにD-Ala-D-Lacを含むペプチドグリカン前駆体を基質として、stem pentapeptideに分枝アミノ酸(Gly)を付加する。
通常のD-Ala-D-Alaを含む前駆体を基質とするFemXはD-Ala-D-Lacを含む前駆体を基質として認識できないため、vancomycin耐性が誘導されたとき、VanKの存在は細胞壁合成のために必須となる。

close this sectionSequence

selected fasta
>putative glycyltransferase [StaO]
MSNNSSRALTRTAAGNPVVRPISAADHLAFVRAQRSVSFLQTPAWGRVKTEWRSESLGWF
DGPRLVGAGLVLHRPVPRLDRYTLAYLPEGPVIDWSSEIGAWLDPLAAYLKEHGAFAIRL
GPPVVTDTWDATQIKDGIADPQIRRLTDMPGRWDDPVGARVSSQLKDAGWLPQNPLDGFG
VGHPQFKYVLPLAGRTEDELLRGMNQQWRRNIKKSAKEGVEVTVSDGGPEDLKAFHDLYV
HTAERDRFTPRPLSYFETMFTALRAEDPERIKIYLAHHQGDLVAATVMVRVGSHAVYSYG
ASATAKREVRGSNACQWAMIRDALAADCDVYDLRGITPTLDADDPHVGLVRFKVGTGGQA
VRYVGEWDLPLRPMIYRAFDLYMRRRGH
selected fasta
>putative glycyltransferase [StaO]
ATGTCCAACAATTCCTCCCGCGCACTCACACGAACCGCAGCCGGAAATCCGGTCGTCAGG
CCGATCAGCGCCGCCGATCACTTGGCGTTCGTCCGGGCCCAGCGGTCGGTCAGCTTCCTG
CAGACCCCGGCGTGGGGCCGGGTCAAGACCGAGTGGCGCAGCGAGTCCCTCGGCTGGTTC
GACGGTCCGCGCCTGGTGGGTGCCGGGCTCGTTCTGCACCGCCCGGTACCGCGGCTCGAC
CGGTACACCCTGGCGTATCTGCCCGAGGGCCCGGTCATCGACTGGAGCAGCGAGATCGGT
GCGTGGCTCGATCCGCTGGCGGCATATCTCAAGGAGCACGGCGCGTTCGCGATCCGGCTC
GGTCCGCCGGTGGTCACGGACACCTGGGACGCCACCCAGATCAAGGACGGCATCGCCGAC
CCGCAGATCCGGCGGCTGACCGACATGCCAGGACGGTGGGACGACCCGGTCGGCGCCCGT
GTGAGCAGCCAGCTCAAGGACGCGGGCTGGCTGCCGCAGAACCCGCTGGACGGGTTCGGG
GTCGGGCATCCGCAGTTCAAGTACGTTCTCCCGTTGGCGGGCAGGACCGAGGACGAGCTG
CTGAGAGGCATGAACCAGCAGTGGCGCCGCAACATCAAGAAGTCGGCCAAGGAGGGCGTC
GAGGTCACGGTCAGCGACGGGGGTCCGGAGGACCTGAAGGCGTTTCACGACCTCTACGTC
CACACCGCCGAGCGCGACCGCTTCACACCCCGCCCGCTGAGCTACTTCGAGACCATGTTC
ACGGCACTGCGCGCCGAGGACCCCGAGCGGATCAAGATCTACCTGGCCCACCACCAGGGC
GACCTGGTCGCGGCCACCGTCATGGTCCGCGTCGGATCCCACGCGGTGTACTCCTACGGC
GCCTCCGCCACCGCGAAGCGCGAGGTGCGCGGCTCCAACGCCTGCCAGTGGGCGATGATC
CGTGACGCACTCGCGGCCGACTGCGACGTCTACGACCTGCGCGGCATCACCCCGACCCTC
GACGCCGACGACCCACACGTCGGCCTTGTCCGGTTCAAGGTCGGCACCGGTGGCCAGGCG
GTGCGGTACGTCGGCGAGTGGGACCTGCCGCTGCGGCCGATGATCTACCGGGCCTTCGAC
CTCTACATGAGGCGCCGCGGGCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003447 Methicillin resistance protein (Family)
[22-276] 1.9e-36 PF02388 [281-380] 4.80000000000001e-26 PF02388
PF02388   FemAB
[17-382] PS51191
PS51191   FEMABX
IPR016181 Acyl-CoA N-acyltransferase (Domain)
[20-129] 6.40000000000004e-34 G3DSA:3.40.630.30 [161-198] 6.40000000000004e-34 G3DSA:3.40.630.30 [199-359] 4.30000000000003e-53 G3DSA:3.40.630.30
G3DSA:3.40.630.30   Acyl_CoA_acyltransferase
[17-194] 7.90003079443025e-34 SSF55729 [195-382] 1.10000150671643e-44 SSF55729
SSF55729   Acyl_CoA_acyltransferase
SignalP No significant hit
TMHMM No significant hit
A4793_00280 A4793_00280   A4793_00300 A4793_00300
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