Balhi_00050 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	4,106 / 4,981 / + [in whole cluster] 4,106 / 4,981 / + [in contig]
Location	4106..4981 [in whole cluster] 4106..4981 [in contig]
Type	CDS
Length	876 bp (291 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative prephenate dehydrogenase
Product (GenBank)	putative prephenate dehydrogenase
Gene
Gene (GenBank)	pdh
EC number	1.3.1.-
Keyword	Hpg
Note
Note (GenBank)
Reference	ACC Q799B2 PmId [17693715] The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. (J Mol Microbiol Biotechnol. , 2007) [20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010) comment [PMID: 17693715](2007) vancomycinにとても似たグリコペプチド系抗生物質balhimycinの生合成gene clusterの追加報告。新たにVanS, VanR, VanY, Bbr, Pdh, Tba, Dahpを同定している。 Pdh: prephenate dehydrogenase Zymomonas mobilisでのcyclohexadienyl dehydrogenase(prephenate dehydrogenase)/Q04983への配列相同性から名付けている。 --- [PMID: 20570618](2010) 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。 pdhは追加コピーによる過剰発現でも、deletion mutantでも、balhimycin産生に有意な変化は見られなかった。
Related Reference	ACC Q04983 PmId [7916685] ( , ) comment BLAST id30% Zymomonas mobilis subsp. mobilis_tyrC Protein TyrC Alternative name(s): Arogenate dehydrogenase(ADH,EC 1.3.1.43) Prephenate dehydrogenase(PDH,EC 1.3.1.12) Cyclohexadienyl dehydrogenase E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。 B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。 tyrosineによるfeedback阻害は受けない。
Family/Domain	FD IPR003099 comment [IPR003099] Prephenate dehydrogenase (Domain) Molecular Function GO:0004665 prephenate dehydrogenase (NADP+) activity GO:0008977 prephenate dehydrogenase activity

Sequence

>putative prephenate dehydrogenase [putative prephenate dehydrogenase]
MTIEKALVVGTGLIGTSVALALREKGVAVFLSDVDTEAARLAQVLGAGREWAGEGVDLAV
IAVPPHLVGDRLADLQKQGAARVYTDVASVKADPIADAERLGCDLASYVPGHPLAGRERS
GPAAARAELFSGRPWALCPGPETDAEALRRVRELVSLCGATAVVVGAGEHDSAVALVSHA
PHVVASAVAASLASGDDVALGLAGQGLRDVTRIAAGDPLLWRRILSGNTRPVAGVLERIA
ADLAAAASALRSGDLDEVTDLLRRGVDGHGRIPGQRGGSLPGRNPAGSPGR

>putative prephenate dehydrogenase [putative prephenate dehydrogenase]
GTGACCATCGAGAAAGCGCTTGTCGTCGGTACCGGGCTGATCGGCACCTCGGTGGCGCTG
GCCCTCCGGGAGAAGGGCGTCGCGGTCTTCCTCTCCGACGTCGACACCGAGGCCGCCCGG
CTGGCGCAGGTACTCGGCGCCGGGCGGGAGTGGGCGGGAGAAGGTGTGGATCTGGCGGTG
ATCGCCGTGCCGCCGCACCTGGTGGGGGACCGGCTGGCCGACCTGCAGAAGCAAGGTGCG
GCCCGGGTGTACACCGACGTGGCCAGTGTGAAGGCCGATCCGATCGCCGACGCGGAGCGG
CTCGGGTGTGACCTGGCCTCCTATGTGCCGGGCCACCCGCTTGCCGGCCGGGAACGCTCG
GGCCCGGCCGCCGCCCGCGCCGAGCTGTTCTCGGGCCGGCCGTGGGCACTGTGCCCCGGC
CCCGAGACGGACGCGGAAGCCCTGCGACGGGTGCGGGAGCTGGTGTCCCTGTGCGGGGCG
ACGGCCGTCGTCGTGGGTGCGGGCGAGCACGACTCGGCCGTGGCGCTGGTGTCGCACGCC
CCGCACGTGGTGGCGTCGGCGGTGGCGGCCAGCCTGGCGAGCGGCGACGACGTCGCGCTG
GGCCTGGCGGGGCAGGGACTCCGTGATGTGACGCGCATCGCAGCCGGGGATCCCTTGCTG
TGGCGGAGGATTCTCTCCGGGAACACCCGGCCGGTGGCCGGGGTGCTCGAACGGATCGCG
GCCGACCTCGCCGCGGCGGCCTCGGCGTTGCGGTCCGGCGACCTGGACGAGGTGACGGAT
CTGCTGCGGCGCGGCGTGGACGGTCACGGCCGGATCCCTGGTCAGCGCGGCGGATCCCTT
CCCGGCCGCAACCCGGCGGGTTCCCCGGGGCGTTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003099 Prephenate dehydrogenase (Domain) PF02153 PDH PS51176 PDH_ADH IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Balhi_00040 Balhi_00060