Balhi_00080 Balhi_00080   Balhi_00100 Balhi_00100

Balhi_00090 : CDS information

close this sectionLocation

Organism
StrainDSM 5908
Entry nameBalhimycin
Contig
Start / Stop / Direction28,888 / 34,464 / + [in whole cluster]
28,888 / 34,464 / + [in contig]
Location28888..34464 [in whole cluster]
28888..34464 [in contig]
TypeCDS
Length5,577 bp (1,858 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)peptide synthetase
Gene
Gene (GenBank)bpsC
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11932455] Nonribosomal biosynthesis of vancomycin-type antibiotics: a heptapeptide backbone and eight peptide synthetase modules. (Microbiology. , 2002)
[15651041] The biosynthesis of vancomycin-type glycopeptide antibiotics--a model for oxidative side-chain cross-linking by oxygenases coupled to the action of peptide synthetases. (Chembiochem. , 2005)
[17506888] Phylogenetic analysis of condensation domains in NRPS sheds light on their functional evolution. (BMC Evol Biol. , 2007)
comment
[PMID: 11932455](2002)
balhimycin生合成gene clusterにおけるbpsA, bpsB, bpsC, bpsD, orf1の同定。

BpsCのドメイン構造は、
module 7: C-A-T-C-TE
adenylation(A) domainのselectivity-conferring codeを確認している。

bpsC in-frame deletion mutantは抗菌活性のある化合物を産生しなかった。
module 7 は L-3,5-dihydroxyphenylglycine(Dpg)を活性化することが、異種性発現して得たA domainのATP/PP(i) exchange assaysによって測定された。

thiolation(T) domainとthioesterase(TE) domainの間にあるC domainに似た配列に割り当てられるペプチド骨格構築での機能はない。

---
[PMID: 15651041](2005)
dpgA mutantではbicyclic hexapeptide、bpsC mutantではmonocyclic hexapeptideの蓄積があることから、OxyBによるCD ring形成はBpsB_module 6からBpsC_module 7へのhexapeptideの移動中かその少し前に起こるはずだと述べている。bpsC mutantでbicyclic hexapeptideが検出されないのは、BpsCでのdeletionのせいでOxyAとNRPS複合体の間の相互作用に必須な部位がないからであると説明されうる。

また、dpgA mutantにおいて、7つめのAAとしてDpgの代わりにL-4-hydroxyphenylglycine(Hpg)を含むtricyclic heptapeptideが得られたことから、BpsCとoxygenasesはいくらかの基質寛容性を示す。

---
[PMID: 17506888](2007)
NRPS C domain subtypesの系統発生関係の再構築をしている報告。

グリコペプチド系抗生物質のNRPSに含まれるC domain様区域(X*)は、2つのL-AAsの間のペプチド結合を触媒するC domainのsubtypeに関係し、祖先では追加のmoduleがあったと暗示される。

Figure 4の系統樹にこのentryも含まれる。
Related Reference
ACC
Q70AZ6
NITE
Teicp2_00210
PmId
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[26549530] Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. (Angew Chem Int Ed Engl. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
comment
BLAST id79%
Actinoplanes teichomyceticus ATCC 31121株_tcp ORF12
Non-ribosomal peptide synthetase

---
[PMID: 25686610](2015)
SUPPLEMENTARY INFORMATION内のリストにこのUniProt entryの記載があるが、同グループの他論文(PMID: 25358800, 25586301)ではDSM43866株を使用してATCC 31121株由来のgene nameを採用している混乱があるため、同様の可能性あり。

X-domainの結晶構造解析や相互作用の実験から、X-domainがcytochrome P450(oxy genesでコードされる)による架橋形成に関与することがわかった。

---
[PMID: 26549530](2015) abstract + supporting information
PMID: 25686610の続報。同様にsupporting informationでこのUniProt entryの記載あり。その他タンパクもATCC 31121株のentriesを採用している。

close this sectionPKS/NRPS Module

A7 L-3,5-dihydroxyphenylglycine(Dpg)
C15..310
A493..887
PCP969..1036
X1050..1349
TE1622..1836

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [peptide synthetase]
MTVDDTRAKRRSSVEDVWPLSPLQEGMLYHTALDDDGPDTYTVQTVYGIDGPLDPGLLRA
SWQALVDRHAALRACFRYVSGAQMVQVIAREAEVPWRETDLSGLPDDIAEGEVDRLAADE
VAERLRIEAAPLMKLHLIRLGPDRHRLVHTLHHVLVDGWSMPILHRELAAIYAAGGDASG
LPPTVSYRDYLAWLGRQDKEVARAAWRAELAGLDTPTTVAAPDPARVPDIHTAVVELPAE
LTDGLAQFARGHDLTLNTVVQGAWAVVLAQLAGRDDVVFGATASGRPADLPGVEAMVGQL
LNTLPVRVRLDGGRRAAELFARLQRDQSALMAHQHLGLQDVQAVVGPGAVFDTLVIYENF
PRKGLGRAPGGGLSLVPVKRGRNSSHYPFTLITGPGERMPLILDYDRGLFDPAAAESVVG
ALARVLERLVAEPDVLVGRLTLASEAERALVVEGFNATAGPVPGESVLELFARRVAAAPD
AVAITGAAGANLTYAEVDQASNRLAGYLAVRGVGRGDRVGVAMERSPDLLIAFLAIWKAG
AAYVPVDVEYPAERISFVFDDSGVSTVLCTLATSAVAPGNAIVLDAPETRVAVRDCAAPE
IRPHADDLAYVMYTSGSTGLPKGVAIPHGAVAGLAGDAGWQIGPGDGVLMHATHVFDPSL
YAMWVPLVSGARVLLTEPGVLDAAGVRQAVHRGATFVHLTAGTFRALAETAPECFEGLVE
IGTGGDVVPLQSVENLRRAQPGLRVRNTYGPTETTLCATWLPIEPGEVLGRELPIGHPMT
NRRIYLLDAFLRPVPPGVAGELYIAGTGLAHGYLKSPGLTAGRFVACPFAAGERMYRTGD
RARWTRDGEVVFLGRADDQVKIRGYRVELGEVEAALAAQPGVVEAVVTAREDQPGEKRLV
GYFVSDGGDAGPVEIRRQLALVLPDYLVPIAVVALPGLPVTPNGKVDRRALPAPDLAGHS
PEKAPENETEKVLCALFAEILSIDQVGVDDTFHDLGGSSALAMRLVARIREELGADLPIR
QLFSSPTPAGLARALAAKSRPALEAAQRPDRVPVTARQLRAWLLADPGGETAGLHTSVAL
RLHGRVDVPALAAALGDVAARHEILRTTFPGDAQSVHQHVHDALAVELTPVGVTEEDLPG
LLAERRDLLFDLTRDVPWRCDLFALSDNEHVLHLQVHRILADDDSLDVFFRDLAAAYGAR
REGRVPERAPLALQFADYALWEQRLLTDENEPGSLINEQVAFWRDNLAGLDGETVLPFDR
PRPAVPSRRAGTVALRLEAGPHARLTEAAEPPGADTLEMVHAALAMLLAKLGAGHDVVIG
TALPRDEELFDLEPMIGPFTRALALRTDVSGDPTFLEVVARVQEAGQATGEHLDLPFERI
VELLDLPASLARHPVFQVGLQVDEEDIDGWAAAELPALRTAVEPGGTAAMELDLAVKLTE
RFDDDDNAGGLEGALHYATDLFDEATAESVARRLVRVLEQVAEDPGRRISDLDVFLDDFE
RGRPPIAPARWAGAVPPVVAELAGDGPLGALLLDEQLRPVAPGAVGDLYVTGPAVDAGTA
TLATVPCPFGDEGHRMLHTGLLARKTPAKTLVVVGERRRSSASVKTGDFEILLPLRAGGD
RPPLFCVHASGGLSWNYEPLLRYLPPNQPVYGVQARGLARTEPLPGSVEEMAADYLEQIR
AVQPAGPYHLLGWSLGGRIAQAMARLLEADGERLGLLALLDAYPVYMGRKTTGAASEEAA
LEQRNQQDLDLAGQLVKGVAARSRLEAVMRNLWKVGPRHTRSPFAGDVLLFVATVDRPAH
LPVPVAKASWKEFTSGAVEAHEIPSNHYDMVQSAALGQIGAIVAEKLRSRPEGERTQR
selected fasta
>non-ribosomal peptide synthetase [peptide synthetase]
GTGACCGTTGACGACACTCGCGCGAAGCGCCGCTCCAGCGTCGAGGACGTCTGGCCTCTT
TCGCCGCTGCAGGAGGGAATGCTCTATCACACCGCCCTCGACGACGACGGGCCGGACACC
TACACGGTGCAGACCGTCTACGGCATCGACGGCCCGCTGGACCCGGGGCTCTTGCGGGCG
TCGTGGCAGGCGCTCGTGGACCGGCACGCCGCGCTGCGGGCCTGTTTCCGGTACGTCTCC
GGGGCGCAGATGGTGCAGGTCATCGCGCGGGAGGCCGAGGTTCCCTGGCGCGAGACGGAC
CTTTCCGGGCTGCCGGACGACATCGCCGAGGGCGAGGTTGACCGGCTGGCGGCGGACGAG
GTGGCCGAGCGGCTGCGCATCGAGGCCGCGCCGCTGATGAAGCTGCACCTGATCCGGCTC
GGCCCGGACCGCCACCGGCTCGTGCACACGCTGCACCACGTGCTGGTGGACGGCTGGTCG
ATGCCGATCCTGCACCGGGAGCTCGCCGCGATCTACGCGGCGGGCGGGGACGCGTCCGGC
CTCCCGCCCACCGTCTCCTACCGGGACTACCTCGCCTGGCTGGGCCGGCAGGACAAGGAG
GTGGCGCGGGCGGCCTGGCGGGCCGAGCTCGCCGGGCTGGACACGCCGACCACGGTCGCC
GCGCCCGATCCGGCCCGCGTCCCGGACATCCACACGGCGGTGGTCGAGCTGCCGGCGGAG
CTGACGGACGGCTTGGCGCAGTTCGCGCGTGGCCACGACCTCACGCTGAACACCGTCGTG
CAGGGCGCGTGGGCCGTCGTGCTGGCCCAGCTCGCGGGCCGCGACGACGTCGTGTTCGGC
GCGACCGCCTCCGGGCGGCCCGCGGACCTGCCCGGGGTGGAGGCGATGGTCGGCCAGCTG
CTCAACACCCTGCCGGTGCGGGTCCGGCTCGACGGCGGGCGCCGCGCGGCCGAGCTGTTC
GCCCGGCTGCAGCGCGACCAGTCGGCACTCATGGCCCACCAGCACCTCGGCCTGCAGGAC
GTGCAGGCCGTCGTCGGACCCGGAGCGGTCTTCGACACGCTCGTCATCTACGAGAACTTC
CCCCGCAAGGGACTCGGCCGGGCACCGGGCGGTGGCCTGAGCCTGGTCCCGGTGAAGCGC
GGGCGGAACTCCTCGCACTACCCGTTCACGCTGATCACCGGACCCGGCGAGCGGATGCCG
CTGATCCTCGACTACGACCGGGGCCTGTTCGACCCCGCGGCCGCCGAATCGGTCGTCGGC
GCGCTGGCCAGGGTGCTGGAGCGGCTGGTCGCCGAGCCCGACGTCCTCGTCGGCAGGCTG
ACGCTCGCGAGCGAGGCCGAACGCGCGCTGGTGGTGGAGGGCTTCAACGCCACCGCGGGC
CCGGTGCCGGGGGAGTCCGTCCTCGAGCTGTTCGCCCGGCGGGTGGCCGCCGCGCCGGAC
GCGGTGGCGATCACCGGCGCCGCCGGCGCGAACCTGACCTACGCCGAGGTCGACCAGGCG
TCGAACCGGCTGGCGGGCTACCTCGCCGTCCGGGGCGTGGGCCGTGGCGACCGCGTCGGG
GTGGCCATGGAACGGTCGCCGGATCTGCTGATCGCGTTCCTGGCGATCTGGAAGGCGGGT
GCCGCCTACGTTCCGGTGGACGTCGAGTACCCGGCCGAGCGGATCTCGTTCGTCTTCGAC
GACTCCGGCGTCTCGACCGTCCTGTGCACCCTGGCCACCAGCGCGGTCGCGCCGGGCAAC
GCGATCGTGCTCGACGCGCCCGAAACACGCGTGGCCGTGCGGGACTGCGCCGCGCCGGAA
ATCCGGCCGCACGCGGACGACCTGGCGTACGTCATGTACACCTCCGGCTCCACCGGCCTG
CCGAAGGGCGTGGCCATCCCGCACGGGGCCGTGGCCGGCCTGGCGGGCGACGCGGGCTGG
CAGATCGGTCCCGGCGACGGCGTGCTGATGCACGCGACGCACGTCTTCGACCCTTCGCTC
TACGCGATGTGGGTGCCGCTCGTCTCGGGCGCCCGGGTCCTGCTCACCGAGCCGGGGGTG
CTGGACGCGGCCGGGGTACGGCAGGCCGTGCACCGGGGCGCGACCTTCGTCCACCTCACC
GCCGGCACCTTCCGCGCGCTGGCGGAGACGGCACCGGAGTGCTTCGAAGGCCTGGTCGAG
ATCGGGACCGGCGGCGACGTGGTTCCGCTGCAGTCGGTGGAGAACCTGCGGCGGGCCCAG
CCCGGCCTGCGGGTGCGCAACACCTACGGGCCGACCGAGACCACCCTGTGCGCGACGTGG
CTGCCGATCGAGCCCGGTGAGGTGCTCGGCCGGGAGCTGCCGATCGGCCATCCGATGACC
AACCGCCGGATCTACCTCCTCGACGCCTTCCTGCGCCCGGTTCCGCCGGGCGTGGCCGGC
GAGCTGTACATCGCGGGCACGGGCCTGGCCCACGGGTACCTGAAGAGCCCCGGCCTGACG
GCCGGCCGGTTCGTGGCCTGCCCGTTCGCCGCCGGTGAACGCATGTACCGCACCGGCGAC
CGGGCGCGCTGGACCCGCGACGGCGAGGTGGTGTTCCTCGGCCGCGCCGACGACCAGGTG
AAGATCCGCGGCTACCGGGTCGAGCTCGGCGAAGTGGAGGCTGCGCTGGCGGCCCAGCCG
GGCGTGGTCGAGGCCGTCGTCACGGCGCGGGAGGACCAGCCCGGCGAGAAGCGCCTGGTC
GGCTACTTCGTCTCCGACGGCGGCGACGCGGGGCCGGTGGAGATCCGGCGGCAGCTGGCC
CTGGTGCTGCCCGACTACCTGGTCCCCATCGCCGTGGTCGCCCTGCCCGGCCTGCCCGTC
ACCCCCAACGGCAAGGTCGATCGCCGGGCCCTGCCCGCCCCGGATCTCGCGGGACACTCG
CCGGAGAAGGCACCCGAGAACGAGACCGAGAAGGTGCTGTGCGCGCTGTTCGCCGAGATC
CTCAGCATCGACCAGGTGGGGGTCGACGACACCTTCCACGACCTCGGCGGCAGTTCGGCG
CTGGCCATGCGGCTCGTCGCGCGGATCCGTGAGGAGCTCGGCGCGGACCTGCCCATCCGG
CAGCTGTTCTCCTCGCCGACCCCCGCGGGCCTGGCCAGGGCGCTGGCCGCGAAGTCACGC
CCCGCGCTGGAAGCCGCCCAGCGGCCGGACCGGGTGCCCGTCACCGCCCGGCAGCTGCGT
GCCTGGCTGCTGGCCGATCCCGGCGGGGAGACGGCCGGCCTGCACACCTCCGTCGCCCTG
CGCCTGCACGGCCGGGTGGACGTGCCCGCGCTGGCGGCGGCGCTCGGCGACGTCGCGGCC
CGGCACGAGATCCTCCGCACGACCTTCCCGGGTGACGCGCAGAGCGTTCACCAGCACGTC
CACGACGCCTTGGCGGTCGAGCTGACTCCGGTCGGAGTCACCGAGGAAGACCTCCCGGGG
CTGCTCGCCGAGCGGCGTGACCTGCTCTTCGACCTCACCAGGGACGTGCCGTGGCGGTGT
GACCTCTTCGCGCTCTCGGACAACGAGCACGTGCTGCACCTGCAGGTCCACCGGATCCTC
GCCGACGACGACTCGCTCGACGTGTTCTTCCGCGACCTGGCGGCCGCCTATGGTGCGCGC
CGCGAAGGCCGGGTCCCGGAGCGCGCGCCCCTGGCGTTGCAGTTCGCCGACTACGCGCTC
TGGGAGCAGCGCCTGCTCACGGACGAGAACGAGCCGGGCAGCCTGATCAACGAGCAGGTG
GCCTTCTGGCGGGACAACCTGGCCGGCCTCGACGGGGAGACGGTGCTGCCGTTCGACCGC
CCGCGCCCGGCCGTCCCGTCGCGGCGCGCCGGAACGGTCGCGCTGCGGCTGGAGGCCGGC
CCGCACGCCCGGTTGACGGAGGCGGCGGAGCCGCCGGGCGCGGACACGCTCGAGATGGTG
CACGCCGCGCTCGCGATGCTGCTGGCCAAGCTCGGAGCGGGCCACGACGTGGTGATCGGC
ACGGCGCTGCCGCGGGACGAGGAGCTCTTCGACCTCGAGCCGATGATCGGGCCGTTCACC
CGGGCGCTCGCCCTGCGCACCGACGTCTCGGGCGATCCGACCTTCCTCGAGGTCGTCGCC
AGGGTGCAGGAGGCGGGCCAAGCCACGGGCGAGCACCTGGACCTGCCCTTCGAACGGATC
GTCGAGCTGCTCGATCTGCCGGCCTCGCTCGCCCGCCACCCCGTGTTCCAGGTGGGACTT
CAGGTGGACGAGGAGGACATCGACGGATGGGCCGCGGCGGAACTGCCCGCCCTGCGCACC
GCCGTCGAACCCGGCGGGACCGCGGCCATGGAGCTGGACCTCGCGGTCAAGCTCACCGAG
CGCTTCGACGACGACGACAACGCCGGCGGCCTCGAGGGCGCGCTGCACTACGCCACCGAC
CTGTTCGACGAGGCCACGGCGGAGTCGGTGGCCCGGCGGCTGGTCCGCGTCCTCGAGCAG
GTGGCGGAGGATCCCGGGCGGCGGATCAGCGACCTGGATGTCTTCCTGGACGACTTCGAA
CGCGGCCGTCCGCCCATCGCTCCGGCGCGGTGGGCCGGGGCCGTGCCCCCGGTGGTCGCC
GAACTGGCCGGGGACGGCCCGCTCGGCGCGCTCCTGCTCGACGAGCAGCTGCGCCCGGTC
GCTCCCGGAGCCGTCGGCGATCTGTACGTCACCGGCCCGGCCGTGGACGCGGGAACGGCC
ACCCTGGCGACCGTGCCCTGCCCGTTCGGGGACGAGGGGCACCGGATGCTGCACACGGGC
CTGCTCGCCCGCAAAACGCCCGCCAAGACCCTGGTCGTCGTGGGCGAGCGGAGGCGGTCG
AGCGCTTCGGTGAAGACGGGTGACTTCGAGATCCTGCTGCCGCTGCGCGCCGGCGGTGAC
CGCCCGCCCCTGTTCTGCGTCCACGCGAGCGGTGGCCTGAGCTGGAACTACGAGCCGTTG
CTGCGGTACCTCCCGCCGAACCAGCCGGTCTACGGCGTGCAGGCTCGCGGCCTGGCCCGG
ACCGAACCGCTGCCGGGCAGCGTCGAGGAGATGGCGGCCGACTACCTCGAGCAGATCCGT
GCCGTGCAGCCGGCCGGGCCGTACCACCTCCTCGGCTGGTCCCTCGGCGGCCGGATCGCG
CAGGCGATGGCCAGGTTGCTCGAGGCGGACGGGGAGCGGCTCGGCCTGCTCGCCCTGCTC
GACGCCTATCCCGTCTACATGGGACGCAAGACGACCGGCGCCGCGAGCGAAGAAGCGGCT
CTCGAACAGCGGAACCAGCAGGATCTGGACCTCGCGGGGCAACTGGTCAAGGGTGTGGCC
GCCCGGTCGCGCCTCGAGGCGGTCATGCGCAACCTCTGGAAGGTCGGGCCACGGCACACA
CGTTCGCCCTTCGCCGGCGACGTCCTGCTTTTCGTGGCCACTGTGGACCGTCCCGCGCAT
TTGCCCGTCCCAGTGGCGAAGGCCAGCTGGAAGGAATTCACCAGTGGGGCGGTAGAGGCC
CACGAAATCCCGTCCAACCACTACGACATGGTGCAATCCGCGGCGCTGGGCCAGATTGGT
GCCATCGTCGCCGAGAAACTCCGGTCCCGGCCGGAGGGTGAAAGGACACAACGATGA
[7] C15..310
[7] A493..887
[7] L-3,5-dihydroxyphenylglycine(Dpg)657..757
[7] PCP969..1036
[7] X1050..1349
[7] TE1622..1836
[7] C43..930
[7] A1477..2661
[7] L-3,5-dihydroxyphenylglycine(Dpg)1969..2271
[7] PCP2905..3108
[7] X3148..4047
[7] TE4864..5508

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[493-887] 8.49999999999995e-105 PF00501
PF00501   AMP-binding
IPR001031 Thioesterase (Domain)
[1622-1836] 7.79999999999999e-37 PF00975
PF00975   Thioesterase
IPR001242 Condensation domain (Domain)
[15-310] 6.29999999999998e-65 PF00668 [1050-1349] 5.30000000000001e-49 PF00668
PF00668   Condensation
IPR009081 Acyl carrier protein-like (Domain)
[972-1035] 8.9e-14 PF00550
PF00550   PP-binding
[969-1036] PS50075
PS50075   ACP_DOMAIN
[960-1039] 2.5e-26 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[962-1037] 1.20000117458134e-19 SSF47336
SSF47336   ACP_like
IPR010071 Amino acid adenylation domain (Domain)
[493-887] 3.60000000000002e-124 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[611-622] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
Balhi_00080 Balhi_00080   Balhi_00100 Balhi_00100
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