Chlor_00010 : CDS information

Location

Organism	Amycolatopsis orientalis
Strain	A82846UV37B
Entry name	Chloroeremomycin
Contig	AJ223999
Start / Stop / Direction	255 / 1,358 / + [in whole cluster] 255 / 1,358 / + [in contig]
Location	255..1358 [in whole cluster] 255..1358 [in contig]
Type	CDS
Length	1,104 bp (367 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative prephenate dehydrogenase
Product (GenBank)	PCZA363.1
Gene	pdh ORF1
Gene (GenBank)
EC number	1.3.1.-
Keyword	Hpg
Note
Note (GenBank)	similar to prephenate dehydrogenase
Reference	ACC O52817 PmId [9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998) [11137816] Biosynthesis of L-p-hydroxyphenylglycine, a non-proteinogenic amino acid constituent of peptide antibiotics. (Chem Biol. , 2000) [12596194] Vancomycin assembly: nature's way. (Angew Chem Int Ed Engl. , 2003) comment [PMID: 9545426](1998) chloroeremomycin生合成 gene clusterの報告。 ORF1(367aa): Prephenate dehydrogenase 配列解析のみ。 --- [PMID: 11137816](2000) prephenate dehydrogenaseのhomologであるORF1は、可溶性タンパクとして得られなかったので特徴づけできていない。 prephenateからL-p-hydroxyphenylglycineを導く経路を提唱している。 prephenate ↓　prephenate dehydrogenase (ORF1) p-hydroxyphenylpyruvate ↓　4-hydroxymandelate synthase (ORF21, HmaS) L-p-hydroxymandelate ↓　p-hydroxymandelate oxidase (ORF22, Hmo) p-hydroxybenzoylformate ↓　L-p-hydroxyphenylglycine transaminase (ORF17, HpgT) L-p-hydroxyphenylglycine --- [PMID: 12596194](2003) グリコペプチド系抗生物質の生合成に関するレビュー。 chloroeremomycin-producing (Cep) A. orientalis由来約40genesのうち、明らかにグリコペプチド生合成経路に割り当てられる30genesを、タンパク名、予測された or 知られた機能、酵素活性の補助基質・補因子などと共にリスト化している。 >Orf1 metabolic pathway : Hpg protein name : PD function : prephenate dehydrogenase クローニングはされているが、精製と解析はこれまでされていない。
Related Reference	ACC Q799B2 NITE Balhi_00050 PmId [20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010) comment BLAST id82%, C末足りない Amycolatopsis balhimycina_pdh Putative prephenate dehydrogenase 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。 pdhは追加コピーによる過剰発現でも、deletion mutantでも、balhimycin産生に有意な変化は見られなかった。
	ACC Q04983 PmId [7916685] ( , ) comment BLAST id31%, C末足りない Zymomonas mobilis subsp. mobilis_tyrC Protein TyrC Alternative name(s): Arogenate dehydrogenase(ADH,EC 1.3.1.43) Prephenate dehydrogenase(PDH,EC 1.3.1.12) Cyclohexadienyl dehydrogenase E.coliでtyrCを発現させ、精製し、分子量や活性など見ている。ADHとPDHの活性の割合は3：1で、これらの活性は分離できない。N末にNAD+ binding domainあり。 B. subtilisやS. cerevisiaeのPDH、E.coliやE.herbicolaのbifunctional T-proteinsとはアミノ酸配列の相同性は低い。 tyrosineによるfeedback阻害は受けない。
	ACC P20692 PmId [4321765] ( , ) comment BLAST id25%, T-coffee SCORE=84 Bacillus subtilis (strain 168)_tyrA Prephenate dehydrogenase(EC:1.3.1.12) C末にACT domainあり strain 168, 23のclude extractsを用いて、prephenate DH活性確認。 cofactor: NAD inhibitor: Tyr
Family/Domain	FD IPR003099 comment [IPR003099] Prephenate dehydrogenase (Domain) Molecular Function GO:0004665 prephenate dehydrogenase (NADP+) activity GO:0008977 prephenate dehydrogenase activity

Sequence

>putative prephenate dehydrogenase [PCZA363.1]
MEKVLVVGTGLIGTSAALALREKDVTVYLCDIDADAVRLARELGAGQEWTGQDVDLAVIA
VPPHLVGESLADLQKQGAARAYTDVASVKVDPIADAERLGCDMTCYVPGHPLAGRERSGP
AAARADLFLGRPWALCPGPETEAGAEQLARELVSLCGAKSVTVGARQHDSAVALVSHAPH
VAASAVAASLESGDEVALGLAGQGLRDTTRIAAGDPLLWRMILSGNAQPVAGVLDRIAAD
LVAAASALRSGDLDDVTDLLRRGVDGHGRIPGKHSGPVRDFATVQVVLRDRPGELARLFN
AAGLADVNVEDVRLEHSTGLPVGVAEISVRPQDTDRLADALRFHGWHVPPVPATHQVSQH
RSQGCPR

>putative prephenate dehydrogenase [PCZA363.1]
ATGGAGAAGGTGCTCGTTGTCGGCACTGGACTGATCGGCACCTCCGCGGCGCTGGCACTT
CGCGAGAAGGACGTGACGGTCTATCTCTGCGACATTGACGCGGATGCCGTACGGCTGGCA
CGAGAACTCGGCGCGGGCCAGGAATGGACCGGCCAGGACGTGGACCTGGCGGTGATCGCC
GTGCCGCCGCATCTCGTGGGGGAGTCGCTGGCCGATCTGCAGAAGCAGGGTGCCGCCCGG
GCGTACACCGACGTGGCCAGCGTCAAGGTCGATCCGATCGCTGACGCGGAACGGCTTGGA
TGCGACATGACCTGCTATGTGCCGGGGCACCCGCTCGCCGGGCGGGAGCGATCCGGTCCG
GCCGCGGCACGCGCTGACCTGTTCTTGGGCCGCCCCTGGGCACTTTGTCCCGGCCCTGAA
ACGGAAGCTGGTGCCGAACAGCTGGCGCGGGAGCTGGTGTCGTTGTGCGGGGCGAAGTCC
GTCACGGTGGGCGCGCGCCAGCACGACTCGGCGGTAGCGCTGGTGTCACACGCTCCGCAC
GTGGCGGCATCGGCAGTGGCAGCGAGCCTGGAGAGCGGCGACGAGGTCGCGCTGGGCCTT
GCGGGACAAGGGCTGCGCGACACGACGCGCATCGCCGCCGGGGATCCCTTGCTGTGGCGG
ATGATTCTTTCCGGCAACGCCCAGCCGGTCGCCGGTGTGCTCGACCGGATCGCGGCCGAT
CTCGTCGCCGCGGCCTCGGCGCTGCGGTCGGGTGATCTGGATGACGTGACGGATCTGCTG
CGGCGTGGCGTCGACGGTCACGGCCGGATCCCGGGCAAGCACAGTGGACCGGTGCGCGAT
TTCGCCACGGTTCAAGTGGTTCTGCGGGACCGGCCGGGGGAACTGGCCCGGTTGTTCAAC
GCGGCCGGGCTGGCGGACGTCAACGTGGAGGACGTCCGGCTGGAGCACTCGACCGGCCTG
CCGGTCGGTGTGGCCGAGATTTCCGTGCGCCCGCAGGACACCGACCGGCTCGCCGACGCA
CTGCGGTTCCACGGCTGGCACGTTCCGCCTGTTCCTGCAACGCATCAGGTTTCCCAGCAC
CGGTCCCAGGGTTGTCCCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002912 ACT domain (Domain) PF01842 ACT IPR003099 Prephenate dehydrogenase (Domain) PS51176 PDH_ADH PF02153 PDH IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

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